Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants

This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species.

Bibliographic Details

Main Authors:	Filloux, Denis, Dallot, Sylvie, Delaunay, Agnès, Galzi, Serge, Jacquot, Emmanuel, Roumagnac, Philippe
Format:	book_section biblioteca
Language:	eng
Published:	Springer [Etats-Unis]
Subjects:	H20 - Maladies des plantes, U30 - Méthodes de recherche,
Online Access:	http://agritrop.cirad.fr/576056/ http://agritrop.cirad.fr/576056/1/ID%20576056.pdf
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