Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis
Tolerance of recurrent mechanical wounding and exogenous ethylene is a feature of the rubber tree. Latex harvesting involves tapping of the tree bark and ethephon is applied to increase latex flow. Ethylene is an essential element in controlling latex production. The ethylene signalling pathway leads to the activation of Ethylene Response Factor (ERF) transcription factors. This family has been identified in Hevea brasiliensis. This study set out to understand the regulation of ERF genes during latex harvesting in relation to abiotic stress and hormonal treatments. Analyses of the relative transcript abundance were carried out for 35 HbERF genes in latex, in bark from mature trees and in leaves from juvenile plants under multiple abiotic stresses. Twenty-one HbERF genes were regulated by harvesting stress in laticifers, revealing an overrepresentation of genes in group IX. Transcripts of three HbERF-IX genes from HbERF-IXc4, HbERF-IXc5 and HbERF-IXc6 were dramatically accumulated by combining wounding, methyl jasmonate and ethylene treatments. When an ethylene inhibitor was used, the transcript accumulation for these three genes was halted, showing ethylene-dependent induction. Subcellular localization and transactivation experiments confirmed that several members of HbERF-IX are activator-type transcription factors. This study suggested that latex harvesting induces mechanisms developed for the response to abiotic stress. These mechanisms probably depend on various hormonal signalling pathways. Severalmembers of HbERF-IX could be essential integrators of complex hormonal signalling pathways in Hevea.
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dig-cirad-fr-5759322024-01-28T22:40:01Z http://agritrop.cirad.fr/575932/ http://agritrop.cirad.fr/575932/ Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis. Putranto Riza Arief, Duan Cuifang, Kuswanhadi, Chaidamsari Tetty, Rio Maryannick, Piyatrakul Piyanuch, Herlinawati Eva, Pirrello Julien, Dessailly Florence, Leclercq Julie, Bonnot François, Tang Chaorong, Hu Songnian, Montoro Pascal. 2015. PloS One, 10 (4):e0123618, 26 p.https://doi.org/10.1371/journal.pone.0123618 <https://doi.org/10.1371/journal.pone.0123618> Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis Putranto, Riza Arief Duan, Cuifang Kuswanhadi, Chaidamsari, Tetty Rio, Maryannick Piyatrakul, Piyanuch Herlinawati, Eva Pirrello, Julien Dessailly, Florence Leclercq, Julie Bonnot, François Tang, Chaorong Hu, Songnian Montoro, Pascal eng 2015 PloS One F60 - Physiologie et biochimie végétale F01 - Culture des plantes H50 - Troubles divers des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis latex stress abiotique expression des gènes réponse de la plante éthylène Transcription génique métabolisme régulation physiologique éthéphon substance de croissance végétale stade de développement végétal http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_25446 http://aims.fao.org/aos/agrovoc/c_2679 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_15899 http://aims.fao.org/aos/agrovoc/c_5968 http://aims.fao.org/aos/agrovoc/c_5959 Indonésie http://aims.fao.org/aos/agrovoc/c_3840 Tolerance of recurrent mechanical wounding and exogenous ethylene is a feature of the rubber tree. Latex harvesting involves tapping of the tree bark and ethephon is applied to increase latex flow. Ethylene is an essential element in controlling latex production. The ethylene signalling pathway leads to the activation of Ethylene Response Factor (ERF) transcription factors. This family has been identified in Hevea brasiliensis. This study set out to understand the regulation of ERF genes during latex harvesting in relation to abiotic stress and hormonal treatments. Analyses of the relative transcript abundance were carried out for 35 HbERF genes in latex, in bark from mature trees and in leaves from juvenile plants under multiple abiotic stresses. Twenty-one HbERF genes were regulated by harvesting stress in laticifers, revealing an overrepresentation of genes in group IX. Transcripts of three HbERF-IX genes from HbERF-IXc4, HbERF-IXc5 and HbERF-IXc6 were dramatically accumulated by combining wounding, methyl jasmonate and ethylene treatments. When an ethylene inhibitor was used, the transcript accumulation for these three genes was halted, showing ethylene-dependent induction. Subcellular localization and transactivation experiments confirmed that several members of HbERF-IX are activator-type transcription factors. This study suggested that latex harvesting induces mechanisms developed for the response to abiotic stress. These mechanisms probably depend on various hormonal signalling pathways. Severalmembers of HbERF-IX could be essential integrators of complex hormonal signalling pathways in Hevea. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/575932/1/document_575932.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1371/journal.pone.0123618 10.1371/journal.pone.0123618 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0123618 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1371/journal.pone.0123618 |
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F60 - Physiologie et biochimie végétale F01 - Culture des plantes H50 - Troubles divers des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis latex stress abiotique expression des gènes réponse de la plante éthylène Transcription génique métabolisme régulation physiologique éthéphon substance de croissance végétale stade de développement végétal http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_25446 http://aims.fao.org/aos/agrovoc/c_2679 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_15899 http://aims.fao.org/aos/agrovoc/c_5968 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_3840 F60 - Physiologie et biochimie végétale F01 - Culture des plantes H50 - Troubles divers des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis latex stress abiotique expression des gènes réponse de la plante éthylène Transcription génique métabolisme régulation physiologique éthéphon substance de croissance végétale stade de développement végétal http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_25446 http://aims.fao.org/aos/agrovoc/c_2679 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_15899 http://aims.fao.org/aos/agrovoc/c_5968 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_3840 |
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F60 - Physiologie et biochimie végétale F01 - Culture des plantes H50 - Troubles divers des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis latex stress abiotique expression des gènes réponse de la plante éthylène Transcription génique métabolisme régulation physiologique éthéphon substance de croissance végétale stade de développement végétal http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_25446 http://aims.fao.org/aos/agrovoc/c_2679 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_15899 http://aims.fao.org/aos/agrovoc/c_5968 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_3840 F60 - Physiologie et biochimie végétale F01 - Culture des plantes H50 - Troubles divers des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis latex stress abiotique expression des gènes réponse de la plante éthylène Transcription génique métabolisme régulation physiologique éthéphon substance de croissance végétale stade de développement végétal http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_25446 http://aims.fao.org/aos/agrovoc/c_2679 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_15899 http://aims.fao.org/aos/agrovoc/c_5968 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_3840 Putranto, Riza Arief Duan, Cuifang Kuswanhadi, Chaidamsari, Tetty Rio, Maryannick Piyatrakul, Piyanuch Herlinawati, Eva Pirrello, Julien Dessailly, Florence Leclercq, Julie Bonnot, François Tang, Chaorong Hu, Songnian Montoro, Pascal Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis |
description |
Tolerance of recurrent mechanical wounding and exogenous ethylene is a feature of the rubber tree. Latex harvesting involves tapping of the tree bark and ethephon is applied to increase latex flow. Ethylene is an essential element in controlling latex production. The ethylene signalling pathway leads to the activation of Ethylene Response Factor (ERF) transcription factors. This family has been identified in Hevea brasiliensis. This study set out to understand the regulation of ERF genes during latex harvesting in relation to abiotic stress and hormonal treatments. Analyses of the relative transcript abundance were carried out for 35 HbERF genes in latex, in bark from mature trees and in leaves from juvenile plants under multiple abiotic stresses. Twenty-one HbERF genes were regulated by harvesting stress in laticifers, revealing an overrepresentation of genes in group IX. Transcripts of three HbERF-IX genes from HbERF-IXc4, HbERF-IXc5 and HbERF-IXc6 were dramatically accumulated by combining wounding, methyl jasmonate and ethylene treatments. When an ethylene inhibitor was used, the transcript accumulation for these three genes was halted, showing ethylene-dependent induction. Subcellular localization and transactivation experiments confirmed that several members of HbERF-IX are activator-type transcription factors. This study suggested that latex harvesting induces mechanisms developed for the response to abiotic stress. These mechanisms probably depend on various hormonal signalling pathways. Severalmembers of HbERF-IX could be essential integrators of complex hormonal signalling pathways in Hevea. |
format |
article |
topic_facet |
F60 - Physiologie et biochimie végétale F01 - Culture des plantes H50 - Troubles divers des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis latex stress abiotique expression des gènes réponse de la plante éthylène Transcription génique métabolisme régulation physiologique éthéphon substance de croissance végétale stade de développement végétal http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_25446 http://aims.fao.org/aos/agrovoc/c_2679 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_15899 http://aims.fao.org/aos/agrovoc/c_5968 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_3840 |
author |
Putranto, Riza Arief Duan, Cuifang Kuswanhadi, Chaidamsari, Tetty Rio, Maryannick Piyatrakul, Piyanuch Herlinawati, Eva Pirrello, Julien Dessailly, Florence Leclercq, Julie Bonnot, François Tang, Chaorong Hu, Songnian Montoro, Pascal |
author_facet |
Putranto, Riza Arief Duan, Cuifang Kuswanhadi, Chaidamsari, Tetty Rio, Maryannick Piyatrakul, Piyanuch Herlinawati, Eva Pirrello, Julien Dessailly, Florence Leclercq, Julie Bonnot, François Tang, Chaorong Hu, Songnian Montoro, Pascal |
author_sort |
Putranto, Riza Arief |
title |
Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis |
title_short |
Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis |
title_full |
Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis |
title_fullStr |
Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis |
title_full_unstemmed |
Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis |
title_sort |
ethylene response factors are controlled by multiple harvesting stresses in hevea brasiliensis |
url |
http://agritrop.cirad.fr/575932/ http://agritrop.cirad.fr/575932/1/document_575932.pdf |
work_keys_str_mv |
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