What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands
Pneumocystis fungi represent a highly diversified biological group with numerous species, which display a strong host-specificity suggesting a long co-speciation process. In the present study, the presence and genetic diversity of Pneumocystis organisms was investigated in 203 lung samples from woodmice (Apodemus sylvaticus) collected on western continental Europe and Mediterranean islands. The presence of Pneumocystis DNA was assessed by nested PCR at both large and small mitochondrial subunit (mtLSU and mtSSU) rRNA loci. Direct sequencing of nested PCR products demonstrated a very high variability among woodmouse-derived Pneumocystis organisms with a total number of 30 distinct combined mtLSU and mtSSU sequence types. However, the genetic divergence among these sequence types was very low (up to 3.87%) and the presence of several Pneumocystis species within Apodemus sylvaticus was considered unlikely. The analysis of the genetic structure of woodmouse-derived Pneumocystis revealed two distinct groups. The first one comprised Pneumocystis from woodmice collected in continental Spain, France and Balearic islands. The second one included Pneumocystis from woodmice collected in continental Italy, Corsica and Sicily. These two genetic groups were in accordance with the two lineages currently described within the host species Apodemus sylvaticus. Pneumocystis organisms are emerging as powerful tools for phylogeographic studies in mammals.
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dig-cirad-fr-5759252024-12-19T12:33:20Z http://agritrop.cirad.fr/575925/ http://agritrop.cirad.fr/575925/ What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands. Demanche Christine, Deville Manjula, Michaux Johan, Barriel Véronique, Pinçon Claire, Aliouat-Denis Cécile, Pottier Muriel, Noël Christophe, Viscogliosi Eric, Aliouat El Moukhtar, Dei-Cas Eduardo, Morand Serge, Guillot Jacques. 2015. PloS One, 10 (3):e0120839, 17 p.https://doi.org/10.1371/journal.pone.0120839 <https://doi.org/10.1371/journal.pone.0120839> What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands Demanche, Christine Deville, Manjula Michaux, Johan Barriel, Véronique Pinçon, Claire Aliouat-Denis, Cécile Pottier, Muriel Noël, Christophe Viscogliosi, Eric Aliouat, El Moukhtar Dei-Cas, Eduardo Morand, Serge Guillot, Jacques eng 2015 PLOS PloS One L72 - Organismes nuisibles des animaux L73 - Maladies des animaux S50 - Santé humaine Pneumocystis fungi represent a highly diversified biological group with numerous species, which display a strong host-specificity suggesting a long co-speciation process. In the present study, the presence and genetic diversity of Pneumocystis organisms was investigated in 203 lung samples from woodmice (Apodemus sylvaticus) collected on western continental Europe and Mediterranean islands. The presence of Pneumocystis DNA was assessed by nested PCR at both large and small mitochondrial subunit (mtLSU and mtSSU) rRNA loci. Direct sequencing of nested PCR products demonstrated a very high variability among woodmouse-derived Pneumocystis organisms with a total number of 30 distinct combined mtLSU and mtSSU sequence types. However, the genetic divergence among these sequence types was very low (up to 3.87%) and the presence of several Pneumocystis species within Apodemus sylvaticus was considered unlikely. The analysis of the genetic structure of woodmouse-derived Pneumocystis revealed two distinct groups. The first one comprised Pneumocystis from woodmice collected in continental Spain, France and Balearic islands. The second one included Pneumocystis from woodmice collected in continental Italy, Corsica and Sicily. These two genetic groups were in accordance with the two lineages currently described within the host species Apodemus sylvaticus. Pneumocystis organisms are emerging as powerful tools for phylogeographic studies in mammals. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/575925/1/document_575925.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1371/journal.pone.0120839 10.1371/journal.pone.0120839 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0120839 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1371/journal.pone.0120839 |
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L72 - Organismes nuisibles des animaux L73 - Maladies des animaux S50 - Santé humaine L72 - Organismes nuisibles des animaux L73 - Maladies des animaux S50 - Santé humaine |
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L72 - Organismes nuisibles des animaux L73 - Maladies des animaux S50 - Santé humaine L72 - Organismes nuisibles des animaux L73 - Maladies des animaux S50 - Santé humaine Demanche, Christine Deville, Manjula Michaux, Johan Barriel, Véronique Pinçon, Claire Aliouat-Denis, Cécile Pottier, Muriel Noël, Christophe Viscogliosi, Eric Aliouat, El Moukhtar Dei-Cas, Eduardo Morand, Serge Guillot, Jacques What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands |
| description |
Pneumocystis fungi represent a highly diversified biological group with numerous species, which display a strong host-specificity suggesting a long co-speciation process. In the present study, the presence and genetic diversity of Pneumocystis organisms was investigated in 203 lung samples from woodmice (Apodemus sylvaticus) collected on western continental Europe and Mediterranean islands. The presence of Pneumocystis DNA was assessed by nested PCR at both large and small mitochondrial subunit (mtLSU and mtSSU) rRNA loci. Direct sequencing of nested PCR products demonstrated a very high variability among woodmouse-derived Pneumocystis organisms with a total number of 30 distinct combined mtLSU and mtSSU sequence types. However, the genetic divergence among these sequence types was very low (up to 3.87%) and the presence of several Pneumocystis species within Apodemus sylvaticus was considered unlikely. The analysis of the genetic structure of woodmouse-derived Pneumocystis revealed two distinct groups. The first one comprised Pneumocystis from woodmice collected in continental Spain, France and Balearic islands. The second one included Pneumocystis from woodmice collected in continental Italy, Corsica and Sicily. These two genetic groups were in accordance with the two lineages currently described within the host species Apodemus sylvaticus. Pneumocystis organisms are emerging as powerful tools for phylogeographic studies in mammals. |
| format |
article |
| topic_facet |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux S50 - Santé humaine |
| author |
Demanche, Christine Deville, Manjula Michaux, Johan Barriel, Véronique Pinçon, Claire Aliouat-Denis, Cécile Pottier, Muriel Noël, Christophe Viscogliosi, Eric Aliouat, El Moukhtar Dei-Cas, Eduardo Morand, Serge Guillot, Jacques |
| author_facet |
Demanche, Christine Deville, Manjula Michaux, Johan Barriel, Véronique Pinçon, Claire Aliouat-Denis, Cécile Pottier, Muriel Noël, Christophe Viscogliosi, Eric Aliouat, El Moukhtar Dei-Cas, Eduardo Morand, Serge Guillot, Jacques |
| author_sort |
Demanche, Christine |
| title |
What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands |
| title_short |
What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands |
| title_full |
What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands |
| title_fullStr |
What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands |
| title_full_unstemmed |
What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands |
| title_sort |
what do pneumocystis organisms tell us about the phylogeography of their hosts? the case of the woodmouse apodemus sylvaticus in continental europe and western mediterranean islands |
| publisher |
PLOS |
| url |
http://agritrop.cirad.fr/575925/ http://agritrop.cirad.fr/575925/1/document_575925.pdf |
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