Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach
Newcastle disease (ND) is an endemic disease in village chickens in Ethiopia with substantial economic importance. The sensitivity (Se) and specificity (Sp) of a blocking enzyme-linked immunosorbent assay (bELISA, Svanova Biotech), indirect ELISA (iELISA, Laboratoire Service International) and haemagglutination inhibition (HI) test for ND virus (NDV) antibody detection were evaluated in a Bayesian framework in the absence of a gold standard test, on sera collected from unvaccinated chickens kept under the village production system in household flocks and at markets in two woredas (i.e. districts) of the Eastern Shewa zone, Ethiopia. The outcomes of the iELISA test differed dramatically from those of the two other tests with 92% of the samples testing positive as compared with less than 15% for bELISA and HI. iELISA results were also inconsistent with previous estimations of Newcastle serological prevalence. The information provided by the iELISA test was thus considered as highly unreliable, probably due to an extremely low specificity, and thus not considered in the Bayesian models aiming at estimating serological prevalence and test performance parameters. Bayesian modelling of HI and bELISA test results suggested that bELISA had both the highest Se (86.6%; 95% posterior credible interval (PCI): 61.8%; 98.5%), and the highest Sp (98.3%; 95% PCI: 97.2%; 99.5%), while HI had a Se of 80.2% (95% PCI: 59.1%; 94.3%), and a Sp of 96.1% (95% PCI: 95.1%; 97.4%). Model selection and the range of the posterior distribution of the correlation between bELISA and HI test outcomes for truly seropositive animals (median at 0.461; PCI: ?0.055; 0.894) suggested a tendency for bELISA and HI to detect the same truly positive animals and to fail to detect the same truly positive animals. The use of bELISA in screening and surveillance for NDV antibodies is indicated given its high Se and Sp, in addition to its ease of automation to handle large numbers of samples compared to HI. The latter can be used as confirmatory test where an ELISA test with moderate or low specificity is used although the likely positive dependence with bELISA implies that HI and bELISA provide similar information on truly positive animals. Evaluation of commercial ELISAs is indicated before their wider use in village chicken populations to avoid erroneous inferences.
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L73 - Maladies des animaux virus maladie de newcastle test biologique Test ELISA technique immunoenzymatique agglutination immunologique hémagglutinine modèle mathématique poulet anticorps identification immunologie Paramyxovirus aviaire http://aims.fao.org/aos/agrovoc/c_5166 http://aims.fao.org/aos/agrovoc/c_15731 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_16122 http://aims.fao.org/aos/agrovoc/c_8661 http://aims.fao.org/aos/agrovoc/c_35138 http://aims.fao.org/aos/agrovoc/c_24199 http://aims.fao.org/aos/agrovoc/c_1540 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_16388 http://aims.fao.org/aos/agrovoc/c_2676 L73 - Maladies des animaux virus maladie de newcastle test biologique Test ELISA technique immunoenzymatique agglutination immunologique hémagglutinine modèle mathématique poulet anticorps identification immunologie Paramyxovirus aviaire http://aims.fao.org/aos/agrovoc/c_5166 http://aims.fao.org/aos/agrovoc/c_15731 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_16122 http://aims.fao.org/aos/agrovoc/c_8661 http://aims.fao.org/aos/agrovoc/c_35138 http://aims.fao.org/aos/agrovoc/c_24199 http://aims.fao.org/aos/agrovoc/c_1540 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_16388 http://aims.fao.org/aos/agrovoc/c_2676 |
spellingShingle |
L73 - Maladies des animaux virus maladie de newcastle test biologique Test ELISA technique immunoenzymatique agglutination immunologique hémagglutinine modèle mathématique poulet anticorps identification immunologie Paramyxovirus aviaire http://aims.fao.org/aos/agrovoc/c_5166 http://aims.fao.org/aos/agrovoc/c_15731 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_16122 http://aims.fao.org/aos/agrovoc/c_8661 http://aims.fao.org/aos/agrovoc/c_35138 http://aims.fao.org/aos/agrovoc/c_24199 http://aims.fao.org/aos/agrovoc/c_1540 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_16388 http://aims.fao.org/aos/agrovoc/c_2676 L73 - Maladies des animaux virus maladie de newcastle test biologique Test ELISA technique immunoenzymatique agglutination immunologique hémagglutinine modèle mathématique poulet anticorps identification immunologie Paramyxovirus aviaire http://aims.fao.org/aos/agrovoc/c_5166 http://aims.fao.org/aos/agrovoc/c_15731 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_16122 http://aims.fao.org/aos/agrovoc/c_8661 http://aims.fao.org/aos/agrovoc/c_35138 http://aims.fao.org/aos/agrovoc/c_24199 http://aims.fao.org/aos/agrovoc/c_1540 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_16388 http://aims.fao.org/aos/agrovoc/c_2676 Chaka, Hassen Thompson, Peter Goutard, Flavie Grosbois, Vladimir Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach |
description |
Newcastle disease (ND) is an endemic disease in village chickens in Ethiopia with substantial economic importance. The sensitivity (Se) and specificity (Sp) of a blocking enzyme-linked immunosorbent assay (bELISA, Svanova Biotech), indirect ELISA (iELISA, Laboratoire Service International) and haemagglutination inhibition (HI) test for ND virus (NDV) antibody detection were evaluated in a Bayesian framework in the absence of a gold standard test, on sera collected from unvaccinated chickens kept under the village production system in household flocks and at markets in two woredas (i.e. districts) of the Eastern Shewa zone, Ethiopia. The outcomes of the iELISA test differed dramatically from those of the two other tests with 92% of the samples testing positive as compared with less than 15% for bELISA and HI. iELISA results were also inconsistent with previous estimations of Newcastle serological prevalence. The information provided by the iELISA test was thus considered as highly unreliable, probably due to an extremely low specificity, and thus not considered in the Bayesian models aiming at estimating serological prevalence and test performance parameters. Bayesian modelling of HI and bELISA test results suggested that bELISA had both the highest Se (86.6%; 95% posterior credible interval (PCI): 61.8%; 98.5%), and the highest Sp (98.3%; 95% PCI: 97.2%; 99.5%), while HI had a Se of 80.2% (95% PCI: 59.1%; 94.3%), and a Sp of 96.1% (95% PCI: 95.1%; 97.4%). Model selection and the range of the posterior distribution of the correlation between bELISA and HI test outcomes for truly seropositive animals (median at 0.461; PCI: ?0.055; 0.894) suggested a tendency for bELISA and HI to detect the same truly positive animals and to fail to detect the same truly positive animals. The use of bELISA in screening and surveillance for NDV antibodies is indicated given its high Se and Sp, in addition to its ease of automation to handle large numbers of samples compared to HI. The latter can be used as confirmatory test where an ELISA test with moderate or low specificity is used although the likely positive dependence with bELISA implies that HI and bELISA provide similar information on truly positive animals. Evaluation of commercial ELISAs is indicated before their wider use in village chicken populations to avoid erroneous inferences. |
format |
article |
topic_facet |
L73 - Maladies des animaux virus maladie de newcastle test biologique Test ELISA technique immunoenzymatique agglutination immunologique hémagglutinine modèle mathématique poulet anticorps identification immunologie Paramyxovirus aviaire http://aims.fao.org/aos/agrovoc/c_5166 http://aims.fao.org/aos/agrovoc/c_15731 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_16122 http://aims.fao.org/aos/agrovoc/c_8661 http://aims.fao.org/aos/agrovoc/c_35138 http://aims.fao.org/aos/agrovoc/c_24199 http://aims.fao.org/aos/agrovoc/c_1540 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_16388 http://aims.fao.org/aos/agrovoc/c_2676 |
author |
Chaka, Hassen Thompson, Peter Goutard, Flavie Grosbois, Vladimir |
author_facet |
Chaka, Hassen Thompson, Peter Goutard, Flavie Grosbois, Vladimir |
author_sort |
Chaka, Hassen |
title |
Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach |
title_short |
Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach |
title_full |
Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach |
title_fullStr |
Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach |
title_full_unstemmed |
Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach |
title_sort |
evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to newcastle disease virus in village chickens using a bayesian approach |
url |
http://agritrop.cirad.fr/575504/ http://agritrop.cirad.fr/575504/1/document_575504.pdf |
work_keys_str_mv |
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dig-cirad-fr-5755042024-01-28T22:34:03Z http://agritrop.cirad.fr/575504/ http://agritrop.cirad.fr/575504/ Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach. Chaka Hassen, Thompson Peter, Goutard Flavie, Grosbois Vladimir. 2015. Preventive Veterinary Medicine, 119 (1-2) : 21-30.https://doi.org/10.1016/j.prevetmed.2015.01.016 <https://doi.org/10.1016/j.prevetmed.2015.01.016> Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach Chaka, Hassen Thompson, Peter Goutard, Flavie Grosbois, Vladimir eng 2015 Preventive Veterinary Medicine L73 - Maladies des animaux virus maladie de newcastle test biologique Test ELISA technique immunoenzymatique agglutination immunologique hémagglutinine modèle mathématique poulet anticorps identification immunologie Paramyxovirus aviaire http://aims.fao.org/aos/agrovoc/c_5166 http://aims.fao.org/aos/agrovoc/c_15731 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_16122 http://aims.fao.org/aos/agrovoc/c_8661 http://aims.fao.org/aos/agrovoc/c_35138 http://aims.fao.org/aos/agrovoc/c_24199 http://aims.fao.org/aos/agrovoc/c_1540 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_16388 Éthiopie http://aims.fao.org/aos/agrovoc/c_2676 Newcastle disease (ND) is an endemic disease in village chickens in Ethiopia with substantial economic importance. The sensitivity (Se) and specificity (Sp) of a blocking enzyme-linked immunosorbent assay (bELISA, Svanova Biotech), indirect ELISA (iELISA, Laboratoire Service International) and haemagglutination inhibition (HI) test for ND virus (NDV) antibody detection were evaluated in a Bayesian framework in the absence of a gold standard test, on sera collected from unvaccinated chickens kept under the village production system in household flocks and at markets in two woredas (i.e. districts) of the Eastern Shewa zone, Ethiopia. The outcomes of the iELISA test differed dramatically from those of the two other tests with 92% of the samples testing positive as compared with less than 15% for bELISA and HI. iELISA results were also inconsistent with previous estimations of Newcastle serological prevalence. The information provided by the iELISA test was thus considered as highly unreliable, probably due to an extremely low specificity, and thus not considered in the Bayesian models aiming at estimating serological prevalence and test performance parameters. Bayesian modelling of HI and bELISA test results suggested that bELISA had both the highest Se (86.6%; 95% posterior credible interval (PCI): 61.8%; 98.5%), and the highest Sp (98.3%; 95% PCI: 97.2%; 99.5%), while HI had a Se of 80.2% (95% PCI: 59.1%; 94.3%), and a Sp of 96.1% (95% PCI: 95.1%; 97.4%). Model selection and the range of the posterior distribution of the correlation between bELISA and HI test outcomes for truly seropositive animals (median at 0.461; PCI: ?0.055; 0.894) suggested a tendency for bELISA and HI to detect the same truly positive animals and to fail to detect the same truly positive animals. The use of bELISA in screening and surveillance for NDV antibodies is indicated given its high Se and Sp, in addition to its ease of automation to handle large numbers of samples compared to HI. The latter can be used as confirmatory test where an ELISA test with moderate or low specificity is used although the likely positive dependence with bELISA implies that HI and bELISA provide similar information on truly positive animals. Evaluation of commercial ELISAs is indicated before their wider use in village chicken populations to avoid erroneous inferences. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/575504/1/document_575504.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.prevetmed.2015.01.016 10.1016/j.prevetmed.2015.01.016 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.prevetmed.2015.01.016 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.prevetmed.2015.01.016 |