The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice
Polycomb Repressive Complex 2 (PRC2) represses the transcriptional activity of target genes through trimethylation of lysine 27 of histone H3. The functions of plant PRC2 have been chiefly described in Arabidopsis, but specific functions in other plant species, especially cereals, are still largely unknown. Here we characterize mutants in the rice EMF2B gene, an ortholog of the Arabidopsis EMBRYONIC FLOWER2 (EMF2) gene. Loss of EMF2B in rice results in complete sterility, and mutant flowers have severe floral organ defects and indeterminacy that resemble loss-of-function mutants in E-function floral organ specification genes. Transcriptome analysis identified the E-function genes OsMADS1, OsMADS6 and OsMADS34 as differentially expressed in the emf2b mutant compared with wild type. OsMADS1 and OsMADS6, known to be required for meristem determinacy in rice, have reduced expression in the emf2b mutant, whereas OsMADS34 which interacts genetically with OsMADS1 was ectopically expressed. Chromatin immunoprecipitation for H3K27me3 followed by quantitative (q)RT-PCR showed that all three genes are presumptive targets of PRC2 in the meristem. Therefore, in rice, and possibly other cereals, PRC2 appears to play a major role in floral meristem determinacy through modulation of the expression of E-function genes.
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Subjects: | F30 - Génétique et amélioration des plantes, F63 - Physiologie végétale - Reproduction, F50 - Anatomie et morphologie des plantes, Oryza sativa, méristème, inflorescence, physiologie de la reproduction, gène, Transcription génique, expression des gènes, Arabidopsis thaliana, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_4754, http://aims.fao.org/aos/agrovoc/c_3858, http://aims.fao.org/aos/agrovoc/c_13814, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_35128, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_33292, |
Online Access: | http://agritrop.cirad.fr/574720/ http://agritrop.cirad.fr/574720/1/document_574720.pdf |
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dig-cirad-fr-5747202024-01-28T22:22:22Z http://agritrop.cirad.fr/574720/ http://agritrop.cirad.fr/574720/ The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice. Conrad Liza J., Khanday Imtiyaz, Johnson Cameron, Guiderdoni Emmanuel, An Gynheung, Vijayraghavan Usha, Sundaresan Venkatesan. 2014. Plant Journal, 80 (5) : 883-894.https://doi.org/10.1111/tpj.12688 <https://doi.org/10.1111/tpj.12688> The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice Conrad, Liza J. Khanday, Imtiyaz Johnson, Cameron Guiderdoni, Emmanuel An, Gynheung Vijayraghavan, Usha Sundaresan, Venkatesan eng 2014 Plant Journal F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction F50 - Anatomie et morphologie des plantes Oryza sativa méristème inflorescence physiologie de la reproduction gène Transcription génique expression des gènes Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_13814 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_33292 Polycomb Repressive Complex 2 (PRC2) represses the transcriptional activity of target genes through trimethylation of lysine 27 of histone H3. The functions of plant PRC2 have been chiefly described in Arabidopsis, but specific functions in other plant species, especially cereals, are still largely unknown. Here we characterize mutants in the rice EMF2B gene, an ortholog of the Arabidopsis EMBRYONIC FLOWER2 (EMF2) gene. Loss of EMF2B in rice results in complete sterility, and mutant flowers have severe floral organ defects and indeterminacy that resemble loss-of-function mutants in E-function floral organ specification genes. Transcriptome analysis identified the E-function genes OsMADS1, OsMADS6 and OsMADS34 as differentially expressed in the emf2b mutant compared with wild type. OsMADS1 and OsMADS6, known to be required for meristem determinacy in rice, have reduced expression in the emf2b mutant, whereas OsMADS34 which interacts genetically with OsMADS1 was ectopically expressed. Chromatin immunoprecipitation for H3K27me3 followed by quantitative (q)RT-PCR showed that all three genes are presumptive targets of PRC2 in the meristem. Therefore, in rice, and possibly other cereals, PRC2 appears to play a major role in floral meristem determinacy through modulation of the expression of E-function genes. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/574720/1/document_574720.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/tpj.12688 10.1111/tpj.12688 info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.12688 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/tpj.12688 |
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F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction F50 - Anatomie et morphologie des plantes Oryza sativa méristème inflorescence physiologie de la reproduction gène Transcription génique expression des gènes Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_13814 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_33292 F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction F50 - Anatomie et morphologie des plantes Oryza sativa méristème inflorescence physiologie de la reproduction gène Transcription génique expression des gènes Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_13814 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_33292 |
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F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction F50 - Anatomie et morphologie des plantes Oryza sativa méristème inflorescence physiologie de la reproduction gène Transcription génique expression des gènes Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_13814 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_33292 F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction F50 - Anatomie et morphologie des plantes Oryza sativa méristème inflorescence physiologie de la reproduction gène Transcription génique expression des gènes Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_13814 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_33292 Conrad, Liza J. Khanday, Imtiyaz Johnson, Cameron Guiderdoni, Emmanuel An, Gynheung Vijayraghavan, Usha Sundaresan, Venkatesan The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice |
description |
Polycomb Repressive Complex 2 (PRC2) represses the transcriptional activity of target genes through trimethylation of lysine 27 of histone H3. The functions of plant PRC2 have been chiefly described in Arabidopsis, but specific functions in other plant species, especially cereals, are still largely unknown. Here we characterize mutants in the rice EMF2B gene, an ortholog of the Arabidopsis EMBRYONIC FLOWER2 (EMF2) gene. Loss of EMF2B in rice results in complete sterility, and mutant flowers have severe floral organ defects and indeterminacy that resemble loss-of-function mutants in E-function floral organ specification genes. Transcriptome analysis identified the E-function genes OsMADS1, OsMADS6 and OsMADS34 as differentially expressed in the emf2b mutant compared with wild type. OsMADS1 and OsMADS6, known to be required for meristem determinacy in rice, have reduced expression in the emf2b mutant, whereas OsMADS34 which interacts genetically with OsMADS1 was ectopically expressed. Chromatin immunoprecipitation for H3K27me3 followed by quantitative (q)RT-PCR showed that all three genes are presumptive targets of PRC2 in the meristem. Therefore, in rice, and possibly other cereals, PRC2 appears to play a major role in floral meristem determinacy through modulation of the expression of E-function genes. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction F50 - Anatomie et morphologie des plantes Oryza sativa méristème inflorescence physiologie de la reproduction gène Transcription génique expression des gènes Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_13814 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_33292 |
author |
Conrad, Liza J. Khanday, Imtiyaz Johnson, Cameron Guiderdoni, Emmanuel An, Gynheung Vijayraghavan, Usha Sundaresan, Venkatesan |
author_facet |
Conrad, Liza J. Khanday, Imtiyaz Johnson, Cameron Guiderdoni, Emmanuel An, Gynheung Vijayraghavan, Usha Sundaresan, Venkatesan |
author_sort |
Conrad, Liza J. |
title |
The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice |
title_short |
The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice |
title_full |
The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice |
title_fullStr |
The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice |
title_full_unstemmed |
The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice |
title_sort |
polycomb group gene emf2b is essential for maintenance of floral meristem determinacy in rice |
url |
http://agritrop.cirad.fr/574720/ http://agritrop.cirad.fr/574720/1/document_574720.pdf |
work_keys_str_mv |
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