Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil
Nickel (Ni)-tolerant ectomycorrhizal Pisolithus albus was isolated from extreme ultramafic soils that are naturally rich in heavy metals. This study aimed to identify the specific molecular mechanisms associated with the response of P. albus to nickel. In presence of high concentration of nickel, P. albus Ni-tolerant isolate showed a low basal accumulation of nickel in its fungal tissues and was able to perform a metal efflux mechanism. Three genes putatively involved in metal efflux were identified from the P. albus transcriptome, and their overexpression was confirmed in the mycelium that was cultivated in vitro in the presence of nickel and in fungal tissues that were sampled in situ. Cloning these genes in yeast provided significant advantages in terms of nickel tolerance (+ 31% Ni EC50) and growth (+ 83% u) compared with controls. Furthermore, nickel efflux was also detected in the transformed yeast cells. Protein sequence analysis indicated that the genes encoded a P-type-ATPase, an ABC transporter and a major facilitator superfamily permease (MFS). This study sheds light on a global mechanism of metal efflux by P. albus cells that supports nickel tolerance. These specific responses to nickel might contribute to the fungal adaptation in ultramafic soil.
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dig-cirad-fr-5742832024-01-28T22:17:19Z http://agritrop.cirad.fr/574283/ http://agritrop.cirad.fr/574283/ Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil. Majorel Clarisse, Hannibal Laure, Ducousso Marc, Lebrun Michel, Jourand Philippe. 2014. Environmental Microbiology Reports, 6 (5) : 510-518.https://doi.org/10.1111/1758-2229.12176 <https://doi.org/10.1111/1758-2229.12176> Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil Majorel, Clarisse Hannibal, Laure Ducousso, Marc Lebrun, Michel Jourand, Philippe eng 2014 Environmental Microbiology Reports P34 - Biologie du sol F30 - Génétique et amélioration des plantes P33 - Chimie et physique du sol P35 - Fertilité du sol P36 - Érosion, conservation et récupération des sols Pisolithus tolérance nickel physiologie Clonage moléculaire biologie moléculaire gène expression des gènes symbiose Mycorhizé type de sol chimique métal lourd ectomycorhize http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_49881 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_35482 Nouvelle-Calédonie France http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 Nickel (Ni)-tolerant ectomycorrhizal Pisolithus albus was isolated from extreme ultramafic soils that are naturally rich in heavy metals. This study aimed to identify the specific molecular mechanisms associated with the response of P. albus to nickel. In presence of high concentration of nickel, P. albus Ni-tolerant isolate showed a low basal accumulation of nickel in its fungal tissues and was able to perform a metal efflux mechanism. Three genes putatively involved in metal efflux were identified from the P. albus transcriptome, and their overexpression was confirmed in the mycelium that was cultivated in vitro in the presence of nickel and in fungal tissues that were sampled in situ. Cloning these genes in yeast provided significant advantages in terms of nickel tolerance (+ 31% Ni EC50) and growth (+ 83% u) compared with controls. Furthermore, nickel efflux was also detected in the transformed yeast cells. Protein sequence analysis indicated that the genes encoded a P-type-ATPase, an ABC transporter and a major facilitator superfamily permease (MFS). This study sheds light on a global mechanism of metal efflux by P. albus cells that supports nickel tolerance. These specific responses to nickel might contribute to the fungal adaptation in ultramafic soil. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/574283/1/document_574283.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/1758-2229.12176 10.1111/1758-2229.12176 info:eu-repo/semantics/altIdentifier/doi/10.1111/1758-2229.12176 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/1758-2229.12176 |
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Francia |
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Europa del Oeste |
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Biblioteca del CIRAD Francia |
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P34 - Biologie du sol F30 - Génétique et amélioration des plantes P33 - Chimie et physique du sol P35 - Fertilité du sol P36 - Érosion, conservation et récupération des sols Pisolithus tolérance nickel physiologie Clonage moléculaire biologie moléculaire gène expression des gènes symbiose Mycorhizé type de sol chimique métal lourd ectomycorhize http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_49881 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_35482 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 P34 - Biologie du sol F30 - Génétique et amélioration des plantes P33 - Chimie et physique du sol P35 - Fertilité du sol P36 - Érosion, conservation et récupération des sols Pisolithus tolérance nickel physiologie Clonage moléculaire biologie moléculaire gène expression des gènes symbiose Mycorhizé type de sol chimique métal lourd ectomycorhize http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_49881 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_35482 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 |
spellingShingle |
P34 - Biologie du sol F30 - Génétique et amélioration des plantes P33 - Chimie et physique du sol P35 - Fertilité du sol P36 - Érosion, conservation et récupération des sols Pisolithus tolérance nickel physiologie Clonage moléculaire biologie moléculaire gène expression des gènes symbiose Mycorhizé type de sol chimique métal lourd ectomycorhize http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_49881 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_35482 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 P34 - Biologie du sol F30 - Génétique et amélioration des plantes P33 - Chimie et physique du sol P35 - Fertilité du sol P36 - Érosion, conservation et récupération des sols Pisolithus tolérance nickel physiologie Clonage moléculaire biologie moléculaire gène expression des gènes symbiose Mycorhizé type de sol chimique métal lourd ectomycorhize http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_49881 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_35482 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 Majorel, Clarisse Hannibal, Laure Ducousso, Marc Lebrun, Michel Jourand, Philippe Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil |
description |
Nickel (Ni)-tolerant ectomycorrhizal Pisolithus albus was isolated from extreme ultramafic soils that are naturally rich in heavy metals. This study aimed to identify the specific molecular mechanisms associated with the response of P. albus to nickel. In presence of high concentration of nickel, P. albus Ni-tolerant isolate showed a low basal accumulation of nickel in its fungal tissues and was able to perform a metal efflux mechanism. Three genes putatively involved in metal efflux were identified from the P. albus transcriptome, and their overexpression was confirmed in the mycelium that was cultivated in vitro in the presence of nickel and in fungal tissues that were sampled in situ. Cloning these genes in yeast provided significant advantages in terms of nickel tolerance (+ 31% Ni EC50) and growth (+ 83% u) compared with controls. Furthermore, nickel efflux was also detected in the transformed yeast cells. Protein sequence analysis indicated that the genes encoded a P-type-ATPase, an ABC transporter and a major facilitator superfamily permease (MFS). This study sheds light on a global mechanism of metal efflux by P. albus cells that supports nickel tolerance. These specific responses to nickel might contribute to the fungal adaptation in ultramafic soil. |
format |
article |
topic_facet |
P34 - Biologie du sol F30 - Génétique et amélioration des plantes P33 - Chimie et physique du sol P35 - Fertilité du sol P36 - Érosion, conservation et récupération des sols Pisolithus tolérance nickel physiologie Clonage moléculaire biologie moléculaire gène expression des gènes symbiose Mycorhizé type de sol chimique métal lourd ectomycorhize http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_49881 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_35482 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Majorel, Clarisse Hannibal, Laure Ducousso, Marc Lebrun, Michel Jourand, Philippe |
author_facet |
Majorel, Clarisse Hannibal, Laure Ducousso, Marc Lebrun, Michel Jourand, Philippe |
author_sort |
Majorel, Clarisse |
title |
Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil |
title_short |
Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil |
title_full |
Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil |
title_fullStr |
Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil |
title_full_unstemmed |
Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil |
title_sort |
evidence of nickel (ni) efflux in ni-tolerant ectomycorhizal pisolithus albus isolated from ultramafic soil |
url |
http://agritrop.cirad.fr/574283/ http://agritrop.cirad.fr/574283/1/document_574283.pdf |
work_keys_str_mv |
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