Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations
Introducing nitrogen-fixing bacteria as an inoculum in association with legume crops is a common practice in agriculture. However, the question of the evolution of these introduced microorganisms remains crucial, both in terms of microbial ecology and agronomy. We explored this question by analyzing the genetic and symbiotic evolution of two Bradyrhizobium strains inoculated on Acacia mangium in Malaysia and Senegal 15 and 5 years, respectively, after their introduction. Based on typing of several loci, we showed that these two strains, although closely related and originally sampled in Australia, evolved differently. One strain was recovered in soil with the same five loci as the original isolate, whereas the symbiotic cluster of the other strain was detected with no trace of the three housekeeping genes of the original inoculum. Moreover, the nitrogen fixation efficiency was variable among these isolates (either recombinant or not), with significantly high, low, or similar efficiencies compared to the two original strains and no significant difference between recombinant and nonrecombinant isolates. These data suggested that 15 years after their introduction, nitrogen-fixing bacteria remain in the soil but that closely related inoculant strains may not evolve in the same way, either genetically or symbiotically. In a context of increasing agronomical use of microbial inoculants (for biological control, nitrogen fixation, or plant growth promotion), this result feeds the debate on the consequences associated with such practices.
id |
dig-cirad-fr-573815 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5738152024-01-28T22:10:59Z http://agritrop.cirad.fr/573815/ http://agritrop.cirad.fr/573815/ Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations. Perrineau Marie-Mathilde, Le Roux Christine, Galiana Antoine, Faye Aliou, Duponnois Robin, Goh Doreen Kim Soh, Prin Yves, Béna Gilles. 2014. Applied and Environmental Microbiology, 80 (18) : 5709-5716.https://doi.org/10.1128/AEM.02007-14 <https://doi.org/10.1128/AEM.02007-14> Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations Perrineau, Marie-Mathilde Le Roux, Christine Galiana, Antoine Faye, Aliou Duponnois, Robin Goh, Doreen Kim Soh Prin, Yves Béna, Gilles eng 2014 Applied and Environmental Microbiology P34 - Biologie du sol K01 - Foresterie - Considérations générales Acacia mangium Bradyrhizobium écologie microbienne génétique des populations dynamique des populations recombinaison bactérie fixatrice de l'azote Fixation de l'azote efficacité inoculation impact sur l'environnement évaluation de l'impact http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_2491 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_24420 http://aims.fao.org/aos/agrovoc/c_37938 Sénégal Malaisie Australie http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_714 Introducing nitrogen-fixing bacteria as an inoculum in association with legume crops is a common practice in agriculture. However, the question of the evolution of these introduced microorganisms remains crucial, both in terms of microbial ecology and agronomy. We explored this question by analyzing the genetic and symbiotic evolution of two Bradyrhizobium strains inoculated on Acacia mangium in Malaysia and Senegal 15 and 5 years, respectively, after their introduction. Based on typing of several loci, we showed that these two strains, although closely related and originally sampled in Australia, evolved differently. One strain was recovered in soil with the same five loci as the original isolate, whereas the symbiotic cluster of the other strain was detected with no trace of the three housekeeping genes of the original inoculum. Moreover, the nitrogen fixation efficiency was variable among these isolates (either recombinant or not), with significantly high, low, or similar efficiencies compared to the two original strains and no significant difference between recombinant and nonrecombinant isolates. These data suggested that 15 years after their introduction, nitrogen-fixing bacteria remain in the soil but that closely related inoculant strains may not evolve in the same way, either genetically or symbiotically. In a context of increasing agronomical use of microbial inoculants (for biological control, nitrogen fixation, or plant growth promotion), this result feeds the debate on the consequences associated with such practices. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/573815/1/document_573815.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1128/AEM.02007-14 10.1128/AEM.02007-14 info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.02007-14 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1128/AEM.02007-14 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
P34 - Biologie du sol K01 - Foresterie - Considérations générales Acacia mangium Bradyrhizobium écologie microbienne génétique des populations dynamique des populations recombinaison bactérie fixatrice de l'azote Fixation de l'azote efficacité inoculation impact sur l'environnement évaluation de l'impact http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_2491 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_24420 http://aims.fao.org/aos/agrovoc/c_37938 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_714 P34 - Biologie du sol K01 - Foresterie - Considérations générales Acacia mangium Bradyrhizobium écologie microbienne génétique des populations dynamique des populations recombinaison bactérie fixatrice de l'azote Fixation de l'azote efficacité inoculation impact sur l'environnement évaluation de l'impact http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_2491 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_24420 http://aims.fao.org/aos/agrovoc/c_37938 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_714 |
spellingShingle |
P34 - Biologie du sol K01 - Foresterie - Considérations générales Acacia mangium Bradyrhizobium écologie microbienne génétique des populations dynamique des populations recombinaison bactérie fixatrice de l'azote Fixation de l'azote efficacité inoculation impact sur l'environnement évaluation de l'impact http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_2491 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_24420 http://aims.fao.org/aos/agrovoc/c_37938 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_714 P34 - Biologie du sol K01 - Foresterie - Considérations générales Acacia mangium Bradyrhizobium écologie microbienne génétique des populations dynamique des populations recombinaison bactérie fixatrice de l'azote Fixation de l'azote efficacité inoculation impact sur l'environnement évaluation de l'impact http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_2491 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_24420 http://aims.fao.org/aos/agrovoc/c_37938 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_714 Perrineau, Marie-Mathilde Le Roux, Christine Galiana, Antoine Faye, Aliou Duponnois, Robin Goh, Doreen Kim Soh Prin, Yves Béna, Gilles Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations |
description |
Introducing nitrogen-fixing bacteria as an inoculum in association with legume crops is a common practice in agriculture. However, the question of the evolution of these introduced microorganisms remains crucial, both in terms of microbial ecology and agronomy. We explored this question by analyzing the genetic and symbiotic evolution of two Bradyrhizobium strains inoculated on Acacia mangium in Malaysia and Senegal 15 and 5 years, respectively, after their introduction. Based on typing of several loci, we showed that these two strains, although closely related and originally sampled in Australia, evolved differently. One strain was recovered in soil with the same five loci as the original isolate, whereas the symbiotic cluster of the other strain was detected with no trace of the three housekeeping genes of the original inoculum. Moreover, the nitrogen fixation efficiency was variable among these isolates (either recombinant or not), with significantly high, low, or similar efficiencies compared to the two original strains and no significant difference between recombinant and nonrecombinant isolates. These data suggested that 15 years after their introduction, nitrogen-fixing bacteria remain in the soil but that closely related inoculant strains may not evolve in the same way, either genetically or symbiotically. In a context of increasing agronomical use of microbial inoculants (for biological control, nitrogen fixation, or plant growth promotion), this result feeds the debate on the consequences associated with such practices. |
format |
article |
topic_facet |
P34 - Biologie du sol K01 - Foresterie - Considérations générales Acacia mangium Bradyrhizobium écologie microbienne génétique des populations dynamique des populations recombinaison bactérie fixatrice de l'azote Fixation de l'azote efficacité inoculation impact sur l'environnement évaluation de l'impact http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_2491 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_24420 http://aims.fao.org/aos/agrovoc/c_37938 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_714 |
author |
Perrineau, Marie-Mathilde Le Roux, Christine Galiana, Antoine Faye, Aliou Duponnois, Robin Goh, Doreen Kim Soh Prin, Yves Béna, Gilles |
author_facet |
Perrineau, Marie-Mathilde Le Roux, Christine Galiana, Antoine Faye, Aliou Duponnois, Robin Goh, Doreen Kim Soh Prin, Yves Béna, Gilles |
author_sort |
Perrineau, Marie-Mathilde |
title |
Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations |
title_short |
Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations |
title_full |
Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations |
title_fullStr |
Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations |
title_full_unstemmed |
Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations |
title_sort |
differing courses of genetic evolution of bradyrhizobium inoculants as revealed by long-term molecular tracing in acacia mangium plantations |
url |
http://agritrop.cirad.fr/573815/ http://agritrop.cirad.fr/573815/1/document_573815.pdf |
work_keys_str_mv |
AT perrineaumariemathilde differingcoursesofgeneticevolutionofbradyrhizobiuminoculantsasrevealedbylongtermmoleculartracinginacaciamangiumplantations AT lerouxchristine differingcoursesofgeneticevolutionofbradyrhizobiuminoculantsasrevealedbylongtermmoleculartracinginacaciamangiumplantations AT galianaantoine differingcoursesofgeneticevolutionofbradyrhizobiuminoculantsasrevealedbylongtermmoleculartracinginacaciamangiumplantations AT fayealiou differingcoursesofgeneticevolutionofbradyrhizobiuminoculantsasrevealedbylongtermmoleculartracinginacaciamangiumplantations AT duponnoisrobin differingcoursesofgeneticevolutionofbradyrhizobiuminoculantsasrevealedbylongtermmoleculartracinginacaciamangiumplantations AT gohdoreenkimsoh differingcoursesofgeneticevolutionofbradyrhizobiuminoculantsasrevealedbylongtermmoleculartracinginacaciamangiumplantations AT prinyves differingcoursesofgeneticevolutionofbradyrhizobiuminoculantsasrevealedbylongtermmoleculartracinginacaciamangiumplantations AT benagilles differingcoursesofgeneticevolutionofbradyrhizobiuminoculantsasrevealedbylongtermmoleculartracinginacaciamangiumplantations |
_version_ |
1792498678221504512 |