Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme
Vanillin is a popular and valuable flavour compound. It is the key constituent of the natural vanilla flavour obtained from cured vanilla pods. Here we show that a single hydratase/lyase type enzyme designated vanillin synthase (VpVAN) catalyses direct conversion of ferulic acid and its glucoside into vanillin and its glucoside, respectively. The enzyme shows high sequence similarity to cysteine proteinases and is specific to the substitution pattern at the aromatic ring and does not metabolize caffeic acid and p-coumaric acid as demonstrated by coupled transcription/translation assays. VpVAN localizes to the inner part of the vanilla pod and high transcript levels are found in single cells located a few cell layers from the inner epidermis. Transient expression of VpVAN in tobacco and stable expression in barley in combination with the action of endogenous alcohol dehydrogenases and UDP-glucosyltransferases result in vanillyl alcohol glucoside formation from endogenous ferulic acid. A gene encoding an enzyme showing 71% sequence identity to VpVAN was identified in another vanillin-producing plant species Glechoma hederacea and was also shown to be a vanillin synthase as demonstrated by transient expression in tobacco.
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Subjects: | F60 - Physiologie et biochimie végétale, Vanilla planifolia, vanilline, acide férulique, biosynthèse, activité enzymatique, ligase, Glechoma, Nicotiana, http://aims.fao.org/aos/agrovoc/c_8156, http://aims.fao.org/aos/agrovoc/c_28610, http://aims.fao.org/aos/agrovoc/c_33987, http://aims.fao.org/aos/agrovoc/c_928, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_4320, http://aims.fao.org/aos/agrovoc/c_37127, http://aims.fao.org/aos/agrovoc/c_5175, http://aims.fao.org/aos/agrovoc/c_6543, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/573566/ http://agritrop.cirad.fr/573566/1/document_573566.pdf |
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dig-cirad-fr-5735662024-01-28T22:06:26Z http://agritrop.cirad.fr/573566/ http://agritrop.cirad.fr/573566/ Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme. Gallage Nethaji J., Hansen Esben H., Kannangara Rubini, Olsen Carl Erik, Motawia Mohammed Saddik, Jorgensen Kirsten, Holme Inger, Hebelstrup Kim, Grisoni Michel, Moller Birger Lindberg. 2014. Nature Communications, 5 (4037), 14 p.https://doi.org/10.1038/ncomms5037 <https://doi.org/10.1038/ncomms5037> Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme Gallage, Nethaji J. Hansen, Esben H. Kannangara, Rubini Olsen, Carl Erik Motawia, Mohammed Saddik Jorgensen, Kirsten Holme, Inger Hebelstrup, Kim Grisoni, Michel Moller, Birger Lindberg eng 2014 Nature Communications F60 - Physiologie et biochimie végétale Vanilla planifolia vanilline acide férulique biosynthèse activité enzymatique ligase Glechoma Nicotiana http://aims.fao.org/aos/agrovoc/c_8156 http://aims.fao.org/aos/agrovoc/c_28610 http://aims.fao.org/aos/agrovoc/c_33987 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_4320 http://aims.fao.org/aos/agrovoc/c_37127 http://aims.fao.org/aos/agrovoc/c_5175 La Réunion France http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Vanillin is a popular and valuable flavour compound. It is the key constituent of the natural vanilla flavour obtained from cured vanilla pods. Here we show that a single hydratase/lyase type enzyme designated vanillin synthase (VpVAN) catalyses direct conversion of ferulic acid and its glucoside into vanillin and its glucoside, respectively. The enzyme shows high sequence similarity to cysteine proteinases and is specific to the substitution pattern at the aromatic ring and does not metabolize caffeic acid and p-coumaric acid as demonstrated by coupled transcription/translation assays. VpVAN localizes to the inner part of the vanilla pod and high transcript levels are found in single cells located a few cell layers from the inner epidermis. Transient expression of VpVAN in tobacco and stable expression in barley in combination with the action of endogenous alcohol dehydrogenases and UDP-glucosyltransferases result in vanillyl alcohol glucoside formation from endogenous ferulic acid. A gene encoding an enzyme showing 71% sequence identity to VpVAN was identified in another vanillin-producing plant species Glechoma hederacea and was also shown to be a vanillin synthase as demonstrated by transient expression in tobacco. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/573566/1/document_573566.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1038/ncomms5037 10.1038/ncomms5037 info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms5037 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1038/ncomms5037 |
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F60 - Physiologie et biochimie végétale Vanilla planifolia vanilline acide férulique biosynthèse activité enzymatique ligase Glechoma Nicotiana http://aims.fao.org/aos/agrovoc/c_8156 http://aims.fao.org/aos/agrovoc/c_28610 http://aims.fao.org/aos/agrovoc/c_33987 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_4320 http://aims.fao.org/aos/agrovoc/c_37127 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 F60 - Physiologie et biochimie végétale Vanilla planifolia vanilline acide férulique biosynthèse activité enzymatique ligase Glechoma Nicotiana http://aims.fao.org/aos/agrovoc/c_8156 http://aims.fao.org/aos/agrovoc/c_28610 http://aims.fao.org/aos/agrovoc/c_33987 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_4320 http://aims.fao.org/aos/agrovoc/c_37127 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
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F60 - Physiologie et biochimie végétale Vanilla planifolia vanilline acide férulique biosynthèse activité enzymatique ligase Glechoma Nicotiana http://aims.fao.org/aos/agrovoc/c_8156 http://aims.fao.org/aos/agrovoc/c_28610 http://aims.fao.org/aos/agrovoc/c_33987 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_4320 http://aims.fao.org/aos/agrovoc/c_37127 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 F60 - Physiologie et biochimie végétale Vanilla planifolia vanilline acide férulique biosynthèse activité enzymatique ligase Glechoma Nicotiana http://aims.fao.org/aos/agrovoc/c_8156 http://aims.fao.org/aos/agrovoc/c_28610 http://aims.fao.org/aos/agrovoc/c_33987 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_4320 http://aims.fao.org/aos/agrovoc/c_37127 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Gallage, Nethaji J. Hansen, Esben H. Kannangara, Rubini Olsen, Carl Erik Motawia, Mohammed Saddik Jorgensen, Kirsten Holme, Inger Hebelstrup, Kim Grisoni, Michel Moller, Birger Lindberg Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme |
description |
Vanillin is a popular and valuable flavour compound. It is the key constituent of the natural vanilla flavour obtained from cured vanilla pods. Here we show that a single hydratase/lyase type enzyme designated vanillin synthase (VpVAN) catalyses direct conversion of ferulic acid and its glucoside into vanillin and its glucoside, respectively. The enzyme shows high sequence similarity to cysteine proteinases and is specific to the substitution pattern at the aromatic ring and does not metabolize caffeic acid and p-coumaric acid as demonstrated by coupled transcription/translation assays. VpVAN localizes to the inner part of the vanilla pod and high transcript levels are found in single cells located a few cell layers from the inner epidermis. Transient expression of VpVAN in tobacco and stable expression in barley in combination with the action of endogenous alcohol dehydrogenases and UDP-glucosyltransferases result in vanillyl alcohol glucoside formation from endogenous ferulic acid. A gene encoding an enzyme showing 71% sequence identity to VpVAN was identified in another vanillin-producing plant species Glechoma hederacea and was also shown to be a vanillin synthase as demonstrated by transient expression in tobacco. |
format |
article |
topic_facet |
F60 - Physiologie et biochimie végétale Vanilla planifolia vanilline acide férulique biosynthèse activité enzymatique ligase Glechoma Nicotiana http://aims.fao.org/aos/agrovoc/c_8156 http://aims.fao.org/aos/agrovoc/c_28610 http://aims.fao.org/aos/agrovoc/c_33987 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_4320 http://aims.fao.org/aos/agrovoc/c_37127 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Gallage, Nethaji J. Hansen, Esben H. Kannangara, Rubini Olsen, Carl Erik Motawia, Mohammed Saddik Jorgensen, Kirsten Holme, Inger Hebelstrup, Kim Grisoni, Michel Moller, Birger Lindberg |
author_facet |
Gallage, Nethaji J. Hansen, Esben H. Kannangara, Rubini Olsen, Carl Erik Motawia, Mohammed Saddik Jorgensen, Kirsten Holme, Inger Hebelstrup, Kim Grisoni, Michel Moller, Birger Lindberg |
author_sort |
Gallage, Nethaji J. |
title |
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme |
title_short |
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme |
title_full |
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme |
title_fullStr |
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme |
title_full_unstemmed |
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme |
title_sort |
vanillin formation from ferulic acid in vanilla planifolia is catalysed by a single enzyme |
url |
http://agritrop.cirad.fr/573566/ http://agritrop.cirad.fr/573566/1/document_573566.pdf |
work_keys_str_mv |
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1792498656410075136 |