Geo-metagenomics: deciphering the spatial biodiversity of ssDNA viruses associated with Western Cape and Camargue agroecosystems
Over the past three years we have developed a geometagenomics approach which, based on the sampling design and the depth of sampling, enables the quantitative ecosystem-scale evaluation of spatial variations in, viral demographics, host distributions, and gene-flow. The approach is particularly well suited to analysing viral dynamics in ecosystem perturbations. The geometagenomics approach can precisely link individual sequence reads from bulked mixed sequencing reactions to information on abiotic and biotic conditions of the samples from which the sequences were obtained, the plant hosts from which samples were collected and the spatial arrangement of the samples. Our experimental design sampling locations are systematically placed within a predefined grid; the location of which is placed according to available geographic information systems data. This a priori choice of the sampling points allows the identification of reference ecosystems that should be appropriate for determining, for example, the impacts of agriculture on viral demographics and evolution within natural endangered ecosystems or the transmission rates of viruses between wild and cultivated plants. Our study was conducted in the Western Cape (South Africa) and Camargue (France) regions, which include wild areas, including renosterveld shrubland and strandveld shrubland besides wide fertile plains under introduced crops such as barley, wheat and wine in South Africa and sansouires, marshes and meadows surrounded by areas under intensive agriculture such as rice and wheat in Camargue. Besides determining the spatial and host distributions of various groups of both known and previously unknown ssDNA viruses, we compare the ssDNA species richness of the various wild and cultivated sampling locations. Amongst a large number of apparently novel single stranded DNA viruses, was one in South Africa belonging to a new Geminivirus genus that we have tentatively named, Capulavirus. (Texte intégral)
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | conference_item biblioteca |
Language: | eng |
Published: |
s.n.
|
Subjects: | H20 - Maladies des plantes, U30 - Méthodes de recherche, |
Online Access: | http://agritrop.cirad.fr/573436/ http://agritrop.cirad.fr/573436/1/document_573436.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-573436 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5734362021-01-04T12:19:59Z http://agritrop.cirad.fr/573436/ http://agritrop.cirad.fr/573436/ Geo-metagenomics: deciphering the spatial biodiversity of ssDNA viruses associated with Western Cape and Camargue agroecosystems. Bernardo Pauline, Ortet Philippe, Barakat Mohamed, Rebelo Anthony, Cousins Stephen, Fernandez Emmanuel, Mesleard François, Peterschmitt Michel, Filloux Denis, Varsani Arvind, Martin Darren Patrick, Roumagnac Philippe. 2013. In : 7th International Geminvirus Symposium and 5th International ssDNA Comparative Virology Workshop, Hangzhou, China, November 3-9, 2013. s.l. : s.n., Résumé, 1 p. International Geminvirus Symposium. 7, Hangzhou, Chine, 3 Novembre 2013/9 Novembre 2013. Researchers Geo-metagenomics: deciphering the spatial biodiversity of ssDNA viruses associated with Western Cape and Camargue agroecosystems Bernardo, Pauline Ortet, Philippe Barakat, Mohamed Rebelo, Anthony Cousins, Stephen Fernandez, Emmanuel Mesleard, François Peterschmitt, Michel Filloux, Denis Varsani, Arvind Martin, Darren Patrick Roumagnac, Philippe eng 2013 s.n. 7th International Geminvirus Symposium and 5th International ssDNA Comparative Virology Workshop, Hangzhou, China, November 3-9, 2013 H20 - Maladies des plantes U30 - Méthodes de recherche Over the past three years we have developed a geometagenomics approach which, based on the sampling design and the depth of sampling, enables the quantitative ecosystem-scale evaluation of spatial variations in, viral demographics, host distributions, and gene-flow. The approach is particularly well suited to analysing viral dynamics in ecosystem perturbations. The geometagenomics approach can precisely link individual sequence reads from bulked mixed sequencing reactions to information on abiotic and biotic conditions of the samples from which the sequences were obtained, the plant hosts from which samples were collected and the spatial arrangement of the samples. Our experimental design sampling locations are systematically placed within a predefined grid; the location of which is placed according to available geographic information systems data. This a priori choice of the sampling points allows the identification of reference ecosystems that should be appropriate for determining, for example, the impacts of agriculture on viral demographics and evolution within natural endangered ecosystems or the transmission rates of viruses between wild and cultivated plants. Our study was conducted in the Western Cape (South Africa) and Camargue (France) regions, which include wild areas, including renosterveld shrubland and strandveld shrubland besides wide fertile plains under introduced crops such as barley, wheat and wine in South Africa and sansouires, marshes and meadows surrounded by areas under intensive agriculture such as rice and wheat in Camargue. Besides determining the spatial and host distributions of various groups of both known and previously unknown ssDNA viruses, we compare the ssDNA species richness of the various wild and cultivated sampling locations. Amongst a large number of apparently novel single stranded DNA viruses, was one in South Africa belonging to a new Geminivirus genus that we have tentatively named, Capulavirus. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/573436/1/document_573436.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H20 - Maladies des plantes U30 - Méthodes de recherche H20 - Maladies des plantes U30 - Méthodes de recherche |
spellingShingle |
H20 - Maladies des plantes U30 - Méthodes de recherche H20 - Maladies des plantes U30 - Méthodes de recherche Bernardo, Pauline Ortet, Philippe Barakat, Mohamed Rebelo, Anthony Cousins, Stephen Fernandez, Emmanuel Mesleard, François Peterschmitt, Michel Filloux, Denis Varsani, Arvind Martin, Darren Patrick Roumagnac, Philippe Geo-metagenomics: deciphering the spatial biodiversity of ssDNA viruses associated with Western Cape and Camargue agroecosystems |
description |
Over the past three years we have developed a geometagenomics approach which, based on the sampling design and the depth of sampling, enables the quantitative ecosystem-scale evaluation of spatial variations in, viral demographics, host distributions, and gene-flow. The approach is particularly well suited to analysing viral dynamics in ecosystem perturbations. The geometagenomics approach can precisely link individual sequence reads from bulked mixed sequencing reactions to information on abiotic and biotic conditions of the samples from which the sequences were obtained, the plant hosts from which samples were collected and the spatial arrangement of the samples. Our experimental design sampling locations are systematically placed within a predefined grid; the location of which is placed according to available geographic information systems data. This a priori choice of the sampling points allows the identification of reference ecosystems that should be appropriate for determining, for example, the impacts of agriculture on viral demographics and evolution within natural endangered ecosystems or the transmission rates of viruses between wild and cultivated plants. Our study was conducted in the Western Cape (South Africa) and Camargue (France) regions, which include wild areas, including renosterveld shrubland and strandveld shrubland besides wide fertile plains under introduced crops such as barley, wheat and wine in South Africa and sansouires, marshes and meadows surrounded by areas under intensive agriculture such as rice and wheat in Camargue. Besides determining the spatial and host distributions of various groups of both known and previously unknown ssDNA viruses, we compare the ssDNA species richness of the various wild and cultivated sampling locations. Amongst a large number of apparently novel single stranded DNA viruses, was one in South Africa belonging to a new Geminivirus genus that we have tentatively named, Capulavirus. (Texte intégral) |
format |
conference_item |
topic_facet |
H20 - Maladies des plantes U30 - Méthodes de recherche |
author |
Bernardo, Pauline Ortet, Philippe Barakat, Mohamed Rebelo, Anthony Cousins, Stephen Fernandez, Emmanuel Mesleard, François Peterschmitt, Michel Filloux, Denis Varsani, Arvind Martin, Darren Patrick Roumagnac, Philippe |
author_facet |
Bernardo, Pauline Ortet, Philippe Barakat, Mohamed Rebelo, Anthony Cousins, Stephen Fernandez, Emmanuel Mesleard, François Peterschmitt, Michel Filloux, Denis Varsani, Arvind Martin, Darren Patrick Roumagnac, Philippe |
author_sort |
Bernardo, Pauline |
title |
Geo-metagenomics: deciphering the spatial biodiversity of ssDNA viruses associated with Western Cape and Camargue agroecosystems |
title_short |
Geo-metagenomics: deciphering the spatial biodiversity of ssDNA viruses associated with Western Cape and Camargue agroecosystems |
title_full |
Geo-metagenomics: deciphering the spatial biodiversity of ssDNA viruses associated with Western Cape and Camargue agroecosystems |
title_fullStr |
Geo-metagenomics: deciphering the spatial biodiversity of ssDNA viruses associated with Western Cape and Camargue agroecosystems |
title_full_unstemmed |
Geo-metagenomics: deciphering the spatial biodiversity of ssDNA viruses associated with Western Cape and Camargue agroecosystems |
title_sort |
geo-metagenomics: deciphering the spatial biodiversity of ssdna viruses associated with western cape and camargue agroecosystems |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/573436/ http://agritrop.cirad.fr/573436/1/document_573436.pdf |
work_keys_str_mv |
AT bernardopauline geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT ortetphilippe geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT barakatmohamed geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT rebeloanthony geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT cousinsstephen geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT fernandezemmanuel geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT mesleardfrancois geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT peterschmittmichel geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT fillouxdenis geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT varsaniarvind geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT martindarrenpatrick geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems AT roumagnacphilippe geometagenomicsdecipheringthespatialbiodiversityofssdnavirusesassociatedwithwesterncapeandcamargueagroecosystems |
_version_ |
1758024181049458688 |