Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots
In this work, we studied the impact of the successive steps of the droplet-vitrification protocol technique employed for cryopreservation of Rubia akane hairy roots on the features of cortical, pericycle and endoderm cells of apical and central root segments, using histology techniques and combining qualitative and quantitative observations. In apical segments, plasmolysis (22-71 %, depending on cell type) was observed only after the loading treatment and did not increase after the following steps of the protocol. By contrast, in central segments, plasmolysis (39-45 %) was already observed after the sucrose pretreatment; it increased to 54-68 %, depending on cell type, after the loading treatment, but no further changes were noted after treatment with the vitrification solution. After liquid nitrogen exposure and unloading treatment, deplasmolysis was more rapid in apical segments, with cortical and pericycle cells having retrieved their original features. In central segments, only cortical cells had retrieved their original features and endoderm and pericycle cells were still highly plasmolysed. Nuclei were more strongly impacted by the cryopreservation protocol in central segments, where they displayed a highly condensed nucleoplasm from the loading treatment onwards and had not retrieved their original aspect after the unloading treatment. By contrast, nuclei had a much less condensed nucleoplasm in cells of apical segments, and they had retrieved their original aspect after the unloading treatment.
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dig-cirad-fr-5732602022-04-15T09:19:31Z http://agritrop.cirad.fr/573260/ http://agritrop.cirad.fr/573260/ Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots. Salma Mohammad, Sylvestre Isabelle, Collin Myriam, Escoute Jacques, Lartaud Marc, Yi Jung-Yoon, Kim Haeng-Hoon, Verdeil Jean-Luc, Engelmann Florent. 2014. Protoplasma, 251 (3) : 649-659.https://doi.org/10.1007/s00709-013-0565-0 <https://doi.org/10.1007/s00709-013-0565-0> Researchers Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots Salma, Mohammad Sylvestre, Isabelle Collin, Myriam Escoute, Jacques Lartaud, Marc Yi, Jung-Yoon Kim, Haeng-Hoon Verdeil, Jean-Luc Engelmann, Florent eng 2014 Protoplasma F01 - Culture des plantes République de Corée http://aims.fao.org/aos/agrovoc/c_4116 In this work, we studied the impact of the successive steps of the droplet-vitrification protocol technique employed for cryopreservation of Rubia akane hairy roots on the features of cortical, pericycle and endoderm cells of apical and central root segments, using histology techniques and combining qualitative and quantitative observations. In apical segments, plasmolysis (22-71 %, depending on cell type) was observed only after the loading treatment and did not increase after the following steps of the protocol. By contrast, in central segments, plasmolysis (39-45 %) was already observed after the sucrose pretreatment; it increased to 54-68 %, depending on cell type, after the loading treatment, but no further changes were noted after treatment with the vitrification solution. After liquid nitrogen exposure and unloading treatment, deplasmolysis was more rapid in apical segments, with cortical and pericycle cells having retrieved their original features. In central segments, only cortical cells had retrieved their original features and endoderm and pericycle cells were still highly plasmolysed. Nuclei were more strongly impacted by the cryopreservation protocol in central segments, where they displayed a highly condensed nucleoplasm from the loading treatment onwards and had not retrieved their original aspect after the unloading treatment. By contrast, nuclei had a much less condensed nucleoplasm in cells of apical segments, and they had retrieved their original aspect after the unloading treatment. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/573260/1/document_573260.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s00709-013-0565-0 10.1007/s00709-013-0565-0 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00709-013-0565-0 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s00709-013-0565-0 |
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F01 - Culture des plantes http://aims.fao.org/aos/agrovoc/c_4116 F01 - Culture des plantes http://aims.fao.org/aos/agrovoc/c_4116 |
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F01 - Culture des plantes http://aims.fao.org/aos/agrovoc/c_4116 F01 - Culture des plantes http://aims.fao.org/aos/agrovoc/c_4116 Salma, Mohammad Sylvestre, Isabelle Collin, Myriam Escoute, Jacques Lartaud, Marc Yi, Jung-Yoon Kim, Haeng-Hoon Verdeil, Jean-Luc Engelmann, Florent Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots |
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In this work, we studied the impact of the successive steps of the droplet-vitrification protocol technique employed for cryopreservation of Rubia akane hairy roots on the features of cortical, pericycle and endoderm cells of apical and central root segments, using histology techniques and combining qualitative and quantitative observations. In apical segments, plasmolysis (22-71 %, depending on cell type) was observed only after the loading treatment and did not increase after the following steps of the protocol. By contrast, in central segments, plasmolysis (39-45 %) was already observed after the sucrose pretreatment; it increased to 54-68 %, depending on cell type, after the loading treatment, but no further changes were noted after treatment with the vitrification solution. After liquid nitrogen exposure and unloading treatment, deplasmolysis was more rapid in apical segments, with cortical and pericycle cells having retrieved their original features. In central segments, only cortical cells had retrieved their original features and endoderm and pericycle cells were still highly plasmolysed. Nuclei were more strongly impacted by the cryopreservation protocol in central segments, where they displayed a highly condensed nucleoplasm from the loading treatment onwards and had not retrieved their original aspect after the unloading treatment. By contrast, nuclei had a much less condensed nucleoplasm in cells of apical segments, and they had retrieved their original aspect after the unloading treatment. |
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article |
topic_facet |
F01 - Culture des plantes http://aims.fao.org/aos/agrovoc/c_4116 |
author |
Salma, Mohammad Sylvestre, Isabelle Collin, Myriam Escoute, Jacques Lartaud, Marc Yi, Jung-Yoon Kim, Haeng-Hoon Verdeil, Jean-Luc Engelmann, Florent |
author_facet |
Salma, Mohammad Sylvestre, Isabelle Collin, Myriam Escoute, Jacques Lartaud, Marc Yi, Jung-Yoon Kim, Haeng-Hoon Verdeil, Jean-Luc Engelmann, Florent |
author_sort |
Salma, Mohammad |
title |
Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots |
title_short |
Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots |
title_full |
Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots |
title_fullStr |
Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots |
title_full_unstemmed |
Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots |
title_sort |
effect of the successive steps of a cryopreservation protocol on the structural integrity of rubia akane nakai hairy roots |
url |
http://agritrop.cirad.fr/573260/ http://agritrop.cirad.fr/573260/1/document_573260.pdf |
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