Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis
Corynespora cassiicola is an important plant pathogenic Ascomycete causing the damaging Corynespora Leaf Fall (CLF) disease in rubber tree (Hevea brasiliensis). A small secreted glycoprotein named cassiicolin was previously described as an important effector of C. cassiicola. In this study, the diversity of the cassiicolin-encoding gene was analysed in C. cassiicola isolates sampled from various hosts and geographical origins. A cassiicolin gene was detected in 47 % of the isolates, encoding up to six distinct protein isoforms. In three isolates, two gene variants encoding cassiicolin isoforms Cas2 and Cas6 were found in the same isolate. A phylogenetic tree based on four combined loci and elucidating the diversity of the whole collection was strongly structured by the toxin class, as defined by the cassiicolin isoform. The isolates carrying the Cas1 gene (toxin class Cas1), all grouped in the same highly supported clade, were found the most aggressive on two rubber tree cultivars. Some isolates in which no Cas gene was detected could nevertheless generate moderate symptoms, suggesting the existence of other yet uncharacterized effectors. This study provides a useful base for future studies of C. cassiicola population biology and epidemiological surveys in various host plants.
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Subjects: | H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes, Hevea brasiliensis, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_4533, http://aims.fao.org/aos/agrovoc/c_1070, |
Online Access: | http://agritrop.cirad.fr/572402/ http://agritrop.cirad.fr/572402/1/document_572402.pdf |
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dig-cirad-fr-5724022024-06-27T16:03:57Z http://agritrop.cirad.fr/572402/ http://agritrop.cirad.fr/572402/ Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis. Déon Marine, Fumanal Boris, Gimenez Stéphanie, Bieysse Daniel, Oliveira Ricardo R., Shuib Siti Shuhada, Breton Frédéric, Elumalai Sunderasan, Vida João B., Seguin Marc, Leroy Thierry, Roeckel-Drevet Patricia, Pujade-Renaud Valérie. 2014. Fungal Biology, 118 (1) : 32-47.https://doi.org/10.1016/j.funbio.2013.10.011 <https://doi.org/10.1016/j.funbio.2013.10.011> Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis Déon, Marine Fumanal, Boris Gimenez, Stéphanie Bieysse, Daniel Oliveira, Ricardo R. Shuib, Siti Shuhada Breton, Frédéric Elumalai, Sunderasan Vida, João B. Seguin, Marc Leroy, Thierry Roeckel-Drevet, Patricia Pujade-Renaud, Valérie eng 2014 Fungal Biology H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_3589 Malaisie Brésil http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_1070 Corynespora cassiicola is an important plant pathogenic Ascomycete causing the damaging Corynespora Leaf Fall (CLF) disease in rubber tree (Hevea brasiliensis). A small secreted glycoprotein named cassiicolin was previously described as an important effector of C. cassiicola. In this study, the diversity of the cassiicolin-encoding gene was analysed in C. cassiicola isolates sampled from various hosts and geographical origins. A cassiicolin gene was detected in 47 % of the isolates, encoding up to six distinct protein isoforms. In three isolates, two gene variants encoding cassiicolin isoforms Cas2 and Cas6 were found in the same isolate. A phylogenetic tree based on four combined loci and elucidating the diversity of the whole collection was strongly structured by the toxin class, as defined by the cassiicolin isoform. The isolates carrying the Cas1 gene (toxin class Cas1), all grouped in the same highly supported clade, were found the most aggressive on two rubber tree cultivars. Some isolates in which no Cas gene was detected could nevertheless generate moderate symptoms, suggesting the existence of other yet uncharacterized effectors. This study provides a useful base for future studies of C. cassiicola population biology and epidemiological surveys in various host plants. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/572402/1/document_572402.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.funbio.2013.10.011 10.1016/j.funbio.2013.10.011 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.funbio.2013.10.011 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.funbio.2013.10.011 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_1070 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_1070 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_1070 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_1070 Déon, Marine Fumanal, Boris Gimenez, Stéphanie Bieysse, Daniel Oliveira, Ricardo R. Shuib, Siti Shuhada Breton, Frédéric Elumalai, Sunderasan Vida, João B. Seguin, Marc Leroy, Thierry Roeckel-Drevet, Patricia Pujade-Renaud, Valérie Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis |
description |
Corynespora cassiicola is an important plant pathogenic Ascomycete causing the damaging Corynespora Leaf Fall (CLF) disease in rubber tree (Hevea brasiliensis). A small secreted glycoprotein named cassiicolin was previously described as an important effector of C. cassiicola. In this study, the diversity of the cassiicolin-encoding gene was analysed in C. cassiicola isolates sampled from various hosts and geographical origins. A cassiicolin gene was detected in 47 % of the isolates, encoding up to six distinct protein isoforms. In three isolates, two gene variants encoding cassiicolin isoforms Cas2 and Cas6 were found in the same isolate. A phylogenetic tree based on four combined loci and elucidating the diversity of the whole collection was strongly structured by the toxin class, as defined by the cassiicolin isoform. The isolates carrying the Cas1 gene (toxin class Cas1), all grouped in the same highly supported clade, were found the most aggressive on two rubber tree cultivars. Some isolates in which no Cas gene was detected could nevertheless generate moderate symptoms, suggesting the existence of other yet uncharacterized effectors. This study provides a useful base for future studies of C. cassiicola population biology and epidemiological surveys in various host plants. |
format |
article |
topic_facet |
H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4533 http://aims.fao.org/aos/agrovoc/c_1070 |
author |
Déon, Marine Fumanal, Boris Gimenez, Stéphanie Bieysse, Daniel Oliveira, Ricardo R. Shuib, Siti Shuhada Breton, Frédéric Elumalai, Sunderasan Vida, João B. Seguin, Marc Leroy, Thierry Roeckel-Drevet, Patricia Pujade-Renaud, Valérie |
author_facet |
Déon, Marine Fumanal, Boris Gimenez, Stéphanie Bieysse, Daniel Oliveira, Ricardo R. Shuib, Siti Shuhada Breton, Frédéric Elumalai, Sunderasan Vida, João B. Seguin, Marc Leroy, Thierry Roeckel-Drevet, Patricia Pujade-Renaud, Valérie |
author_sort |
Déon, Marine |
title |
Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis |
title_short |
Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis |
title_full |
Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis |
title_fullStr |
Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis |
title_full_unstemmed |
Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis |
title_sort |
diversity of the cassiicolin gene in corynespora cassiicola and relation with the pathogenicity in hevea brasiliensis |
url |
http://agritrop.cirad.fr/572402/ http://agritrop.cirad.fr/572402/1/document_572402.pdf |
work_keys_str_mv |
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1806036964381556736 |