Identification of molecular markers associated to new sources of resistance to the root-knot nematode in cotton : S02T04
The root-knot nematode (RKN) Meloidogyne incognita is a major constraint in cotton (Gossypium hirsutum L.) production in numerous countries. Control of RKN has been hampered by the lack of options for crop rotation and even more, by the lack of high-quality locally-adapted varieties exhibiting high levels of resistance. High levels of resistance occur in breeding lines, but the high level of resistance has not readily been transferred to cultivated varieties. Furthermore, cotton genetic improvement is based on the exploitation of a single source of resistance, making the crop vulnerable to the selection of resistant isolates. Resistance to RKN is also found in wild tetraploid cotton (G. hirsutum and G. barbadense) accessions that represent valuable resources for novel genes to be used for cotton improvement. Accessions of Gossypium spp. were evaluated for resistance to RKN in greenhouse experiments, and a highly resistant accession of G. barbadense displaying significant reduction in nematode reproduction was identified. Histological observations of this highly resistant G. barbadense accession (CIR1348) showed that resistance occurs through a two-stage mechanism involving a hypersensitive-like response. Accession CIR1348 showed high levels of resistance to various isolates of RKN originating from the major cotton-producing regions of Brazil. Genetic analyses indicate that resistance is recessive, and controlled by two major genes. The highly resistant accession CIR1348 was crossed with a susceptible cultivated variety (FiberMax966) to produce an F2 mapping population and to initiate the introgression of resistance into an adapted genetic background. The QTL analysis identified 2 major QTLs: one QTL is localized on chromosome 11, previously reported to harbor RKN-resistance loci in other sources of resistance, while the second one resides on a chromosome not previously identified as harboring RKN resistance genes/loci. The identification of SSR markers associated with the 2 major QTLs involved in RKN resistance paves the way to the marker-assisted introgression of the new source of resistance identified in cotton accession CIR1348. The genotyping of BC1 plants is underway to identify those plants harboring the desired allelic combination at the loci of interest. This work is supported by Embrapa (Macroprograma 2 - Convênio Embrapa/Monsanto) and CNPq. (Texte intégral)
Main Authors: | , , , , |
---|---|
Format: | conference_item biblioteca |
Language: | eng |
Published: |
EMBRAPA
|
Subjects: | H10 - Ravageurs des plantes, F30 - Génétique et amélioration des plantes, |
Online Access: | http://agritrop.cirad.fr/571825/ http://agritrop.cirad.fr/571825/1/document_571825.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-571825 |
---|---|
record_format |
koha |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes |
spellingShingle |
H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes Giband, Marc Barroso, Paulo Augusto Vianna Moretzsohn, Márcio C. Lacape, Jean-Marc Carneiro, Regina Maria Dechechi Gomes Identification of molecular markers associated to new sources of resistance to the root-knot nematode in cotton : S02T04 |
description |
The root-knot nematode (RKN) Meloidogyne incognita is a major constraint in cotton (Gossypium hirsutum L.) production in numerous countries. Control of RKN has been hampered by the lack of options for crop rotation and even more, by the lack of high-quality locally-adapted varieties exhibiting high levels of resistance. High levels of resistance occur in breeding lines, but the high level of resistance has not readily been transferred to cultivated varieties. Furthermore, cotton genetic improvement is based on the exploitation of a single source of resistance, making the crop vulnerable to the selection of resistant isolates. Resistance to RKN is also found in wild tetraploid cotton (G. hirsutum and G. barbadense) accessions that represent valuable resources for novel genes to be used for cotton improvement. Accessions of Gossypium spp. were evaluated for resistance to RKN in greenhouse experiments, and a highly resistant accession of G. barbadense displaying significant reduction in nematode reproduction was identified. Histological observations of this highly resistant G. barbadense accession (CIR1348) showed that resistance occurs through a two-stage mechanism involving a hypersensitive-like response. Accession CIR1348 showed high levels of resistance to various isolates of RKN originating from the major cotton-producing regions of Brazil. Genetic analyses indicate that resistance is recessive, and controlled by two major genes. The highly resistant accession CIR1348 was crossed with a susceptible cultivated variety (FiberMax966) to produce an F2 mapping population and to initiate the introgression of resistance into an adapted genetic background. The QTL analysis identified 2 major QTLs: one QTL is localized on chromosome 11, previously reported to harbor RKN-resistance loci in other sources of resistance, while the second one resides on a chromosome not previously identified as harboring RKN resistance genes/loci. The identification of SSR markers associated with the 2 major QTLs involved in RKN resistance paves the way to the marker-assisted introgression of the new source of resistance identified in cotton accession CIR1348. The genotyping of BC1 plants is underway to identify those plants harboring the desired allelic combination at the loci of interest. This work is supported by Embrapa (Macroprograma 2 - Convênio Embrapa/Monsanto) and CNPq. (Texte intégral) |
format |
conference_item |
topic_facet |
H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes |
author |
Giband, Marc Barroso, Paulo Augusto Vianna Moretzsohn, Márcio C. Lacape, Jean-Marc Carneiro, Regina Maria Dechechi Gomes |
author_facet |
Giband, Marc Barroso, Paulo Augusto Vianna Moretzsohn, Márcio C. Lacape, Jean-Marc Carneiro, Regina Maria Dechechi Gomes |
author_sort |
Giband, Marc |
title |
Identification of molecular markers associated to new sources of resistance to the root-knot nematode in cotton : S02T04 |
title_short |
Identification of molecular markers associated to new sources of resistance to the root-knot nematode in cotton : S02T04 |
title_full |
Identification of molecular markers associated to new sources of resistance to the root-knot nematode in cotton : S02T04 |
title_fullStr |
Identification of molecular markers associated to new sources of resistance to the root-knot nematode in cotton : S02T04 |
title_full_unstemmed |
Identification of molecular markers associated to new sources of resistance to the root-knot nematode in cotton : S02T04 |
title_sort |
identification of molecular markers associated to new sources of resistance to the root-knot nematode in cotton : s02t04 |
publisher |
EMBRAPA |
url |
http://agritrop.cirad.fr/571825/ http://agritrop.cirad.fr/571825/1/document_571825.pdf |
work_keys_str_mv |
AT gibandmarc identificationofmolecularmarkersassociatedtonewsourcesofresistancetotherootknotnematodeincottons02t04 AT barrosopauloaugustovianna identificationofmolecularmarkersassociatedtonewsourcesofresistancetotherootknotnematodeincottons02t04 AT moretzsohnmarcioc identificationofmolecularmarkersassociatedtonewsourcesofresistancetotherootknotnematodeincottons02t04 AT lacapejeanmarc identificationofmolecularmarkersassociatedtonewsourcesofresistancetotherootknotnematodeincottons02t04 AT carneiroreginamariadechechigomes identificationofmolecularmarkersassociatedtonewsourcesofresistancetotherootknotnematodeincottons02t04 |
_version_ |
1765903389002039296 |
spelling |
dig-cirad-fr-5718252023-05-09T16:02:52Z http://agritrop.cirad.fr/571825/ http://agritrop.cirad.fr/571825/ Identification of molecular markers associated to new sources of resistance to the root-knot nematode in cotton : S02T04. Giband Marc, Barroso Paulo Augusto Vianna, Moretzsohn Márcio C., Lacape Jean-Marc, Carneiro Regina Maria Dechechi Gomes. 2013. In : Biotic and Abiotic Stress Tolerance in Plants: the Challenge for the 21st Century : Book of abstracts of the CIBA 2013. Brasileiro Ana Christina Miranda (ed.), Fortes Ferreira Claudia (ed.), Fernandez Diana (ed.), Micheli Fabienne (ed.), Coelho Filho M.A. (ed.), Marraccini Pierre (ed.). EMBRAPA, UESC, CIRAD, IRD. Brasilia : EMBRAPA, Résumé, 19-20. Workshop on Biotic and Abiotic Stress Tolerance in Plants: the Challenge for the 21st Century, Ilhéus-Bahia, Brésil, 6 Novembre 2013/8 Novembre 2013.http://www.ciba2013.net/wa_files/Book_20of_20Abstracts.pdf <http://www.ciba2013.net/wa_files/Book_20of_20Abstracts.pdf> Researchers Identification of molecular markers associated to new sources of resistance to the root-knot nematode in cotton : S02T04 Giband, Marc Barroso, Paulo Augusto Vianna Moretzsohn, Márcio C. Lacape, Jean-Marc Carneiro, Regina Maria Dechechi Gomes eng 2013 EMBRAPA Biotic and Abiotic Stress Tolerance in Plants: the Challenge for the 21st Century : Book of abstracts of the CIBA 2013 H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes The root-knot nematode (RKN) Meloidogyne incognita is a major constraint in cotton (Gossypium hirsutum L.) production in numerous countries. Control of RKN has been hampered by the lack of options for crop rotation and even more, by the lack of high-quality locally-adapted varieties exhibiting high levels of resistance. High levels of resistance occur in breeding lines, but the high level of resistance has not readily been transferred to cultivated varieties. Furthermore, cotton genetic improvement is based on the exploitation of a single source of resistance, making the crop vulnerable to the selection of resistant isolates. Resistance to RKN is also found in wild tetraploid cotton (G. hirsutum and G. barbadense) accessions that represent valuable resources for novel genes to be used for cotton improvement. Accessions of Gossypium spp. were evaluated for resistance to RKN in greenhouse experiments, and a highly resistant accession of G. barbadense displaying significant reduction in nematode reproduction was identified. Histological observations of this highly resistant G. barbadense accession (CIR1348) showed that resistance occurs through a two-stage mechanism involving a hypersensitive-like response. Accession CIR1348 showed high levels of resistance to various isolates of RKN originating from the major cotton-producing regions of Brazil. Genetic analyses indicate that resistance is recessive, and controlled by two major genes. The highly resistant accession CIR1348 was crossed with a susceptible cultivated variety (FiberMax966) to produce an F2 mapping population and to initiate the introgression of resistance into an adapted genetic background. The QTL analysis identified 2 major QTLs: one QTL is localized on chromosome 11, previously reported to harbor RKN-resistance loci in other sources of resistance, while the second one resides on a chromosome not previously identified as harboring RKN resistance genes/loci. The identification of SSR markers associated with the 2 major QTLs involved in RKN resistance paves the way to the marker-assisted introgression of the new source of resistance identified in cotton accession CIR1348. The genotyping of BC1 plants is underway to identify those plants harboring the desired allelic combination at the loci of interest. This work is supported by Embrapa (Macroprograma 2 - Convênio Embrapa/Monsanto) and CNPq. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/571825/1/document_571825.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://www.ciba2013.net/wa_files/Book_20of_20Abstracts.pdf http://agritrop.cirad.fr/571812/ info:eu-repo/semantics/altIdentifier/purl/http://www.ciba2013.net/wa_files/Book_20of_20Abstracts.pdf |