Towards a better understanding of how to improve lipase-catalyzed reactions using deep eutectic solvents based on choline chlorid
The new generation of designer solvents, such as deep eutectic solvents (DES), are generally claimed to be renewable and environmentally friendly. Nevertheless, this classification depends entirely on the nature of the selected components. The DES derived from choline chloride (ChCl) combined with a safe hydrogen?bond donor, such as urea, seemed ideal for the design of eco?friendly processes. In mixtures with water, it has been successfully used for lipase?catalyzed reactions of polar substrates. However, no studies have yet examined the role of the different components involved in these mixtures (ChCl, urea, and water) in the medium's functional properties, especially in the biocatalysis field where the enzymes are very sensitive to the environment. By analyzing the water activity (aw) and water content of the solvent, this paper reveals two different thermodynamic areas controlled by the strong association between ChCl and water. In addition, by varying the urea concentration, we show that urea has a denaturing effect on the enzyme and a positive effect on the selectivity of the reaction. Overall, this study made it possible to optimize the lipase?catalyzed alcoholysis reactions, with the synthesis of high yields of a wide range of lipophilic derivative compounds of phenolic acids.
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Subjects: | 000 - Autres thèmes, antioxydant, estérase, biocatalyseur, solvant, lipase, http://aims.fao.org/aos/agrovoc/c_511, http://aims.fao.org/aos/agrovoc/c_2670, http://aims.fao.org/aos/agrovoc/c_27460, http://aims.fao.org/aos/agrovoc/c_7236, http://aims.fao.org/aos/agrovoc/c_12194, |
Online Access: | http://agritrop.cirad.fr/571729/ http://agritrop.cirad.fr/571729/1/document_571729.pdf |
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dig-cirad-fr-5717292024-01-28T21:47:13Z http://agritrop.cirad.fr/571729/ http://agritrop.cirad.fr/571729/ Towards a better understanding of how to improve lipase-catalyzed reactions using deep eutectic solvents based on choline chlorid. Durand Erwann, Lecomte Jérôme, Baréa Bruno, Villeneuve Pierre. 2014. European Journal of Lipid Science and Technology, 116 (1) : 16-23.https://doi.org/10.1002/ejlt.201300246 <https://doi.org/10.1002/ejlt.201300246> Towards a better understanding of how to improve lipase-catalyzed reactions using deep eutectic solvents based on choline chlorid Durand, Erwann Lecomte, Jérôme Baréa, Bruno Villeneuve, Pierre eng 2014 European Journal of Lipid Science and Technology 000 - Autres thèmes antioxydant estérase biocatalyseur solvant lipase http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_27460 http://aims.fao.org/aos/agrovoc/c_7236 http://aims.fao.org/aos/agrovoc/c_12194 The new generation of designer solvents, such as deep eutectic solvents (DES), are generally claimed to be renewable and environmentally friendly. Nevertheless, this classification depends entirely on the nature of the selected components. The DES derived from choline chloride (ChCl) combined with a safe hydrogen?bond donor, such as urea, seemed ideal for the design of eco?friendly processes. In mixtures with water, it has been successfully used for lipase?catalyzed reactions of polar substrates. However, no studies have yet examined the role of the different components involved in these mixtures (ChCl, urea, and water) in the medium's functional properties, especially in the biocatalysis field where the enzymes are very sensitive to the environment. By analyzing the water activity (aw) and water content of the solvent, this paper reveals two different thermodynamic areas controlled by the strong association between ChCl and water. In addition, by varying the urea concentration, we show that urea has a denaturing effect on the enzyme and a positive effect on the selectivity of the reaction. Overall, this study made it possible to optimize the lipase?catalyzed alcoholysis reactions, with the synthesis of high yields of a wide range of lipophilic derivative compounds of phenolic acids. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/571729/1/document_571729.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1002/ejlt.201300246 10.1002/ejlt.201300246 info:eu-repo/semantics/altIdentifier/doi/10.1002/ejlt.201300246 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1002/ejlt.201300246 |
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000 - Autres thèmes antioxydant estérase biocatalyseur solvant lipase http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_27460 http://aims.fao.org/aos/agrovoc/c_7236 http://aims.fao.org/aos/agrovoc/c_12194 000 - Autres thèmes antioxydant estérase biocatalyseur solvant lipase http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_27460 http://aims.fao.org/aos/agrovoc/c_7236 http://aims.fao.org/aos/agrovoc/c_12194 |
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000 - Autres thèmes antioxydant estérase biocatalyseur solvant lipase http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_27460 http://aims.fao.org/aos/agrovoc/c_7236 http://aims.fao.org/aos/agrovoc/c_12194 000 - Autres thèmes antioxydant estérase biocatalyseur solvant lipase http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_27460 http://aims.fao.org/aos/agrovoc/c_7236 http://aims.fao.org/aos/agrovoc/c_12194 Durand, Erwann Lecomte, Jérôme Baréa, Bruno Villeneuve, Pierre Towards a better understanding of how to improve lipase-catalyzed reactions using deep eutectic solvents based on choline chlorid |
description |
The new generation of designer solvents, such as deep eutectic solvents (DES), are generally claimed to be renewable and environmentally friendly. Nevertheless, this classification depends entirely on the nature of the selected components. The DES derived from choline chloride (ChCl) combined with a safe hydrogen?bond donor, such as urea, seemed ideal for the design of eco?friendly processes. In mixtures with water, it has been successfully used for lipase?catalyzed reactions of polar substrates. However, no studies have yet examined the role of the different components involved in these mixtures (ChCl, urea, and water) in the medium's functional properties, especially in the biocatalysis field where the enzymes are very sensitive to the environment. By analyzing the water activity (aw) and water content of the solvent, this paper reveals two different thermodynamic areas controlled by the strong association between ChCl and water. In addition, by varying the urea concentration, we show that urea has a denaturing effect on the enzyme and a positive effect on the selectivity of the reaction. Overall, this study made it possible to optimize the lipase?catalyzed alcoholysis reactions, with the synthesis of high yields of a wide range of lipophilic derivative compounds of phenolic acids. |
format |
article |
topic_facet |
000 - Autres thèmes antioxydant estérase biocatalyseur solvant lipase http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_27460 http://aims.fao.org/aos/agrovoc/c_7236 http://aims.fao.org/aos/agrovoc/c_12194 |
author |
Durand, Erwann Lecomte, Jérôme Baréa, Bruno Villeneuve, Pierre |
author_facet |
Durand, Erwann Lecomte, Jérôme Baréa, Bruno Villeneuve, Pierre |
author_sort |
Durand, Erwann |
title |
Towards a better understanding of how to improve lipase-catalyzed reactions using deep eutectic solvents based on choline chlorid |
title_short |
Towards a better understanding of how to improve lipase-catalyzed reactions using deep eutectic solvents based on choline chlorid |
title_full |
Towards a better understanding of how to improve lipase-catalyzed reactions using deep eutectic solvents based on choline chlorid |
title_fullStr |
Towards a better understanding of how to improve lipase-catalyzed reactions using deep eutectic solvents based on choline chlorid |
title_full_unstemmed |
Towards a better understanding of how to improve lipase-catalyzed reactions using deep eutectic solvents based on choline chlorid |
title_sort |
towards a better understanding of how to improve lipase-catalyzed reactions using deep eutectic solvents based on choline chlorid |
url |
http://agritrop.cirad.fr/571729/ http://agritrop.cirad.fr/571729/1/document_571729.pdf |
work_keys_str_mv |
AT duranderwann towardsabetterunderstandingofhowtoimprovelipasecatalyzedreactionsusingdeepeutecticsolventsbasedoncholinechlorid AT lecomtejerome towardsabetterunderstandingofhowtoimprovelipasecatalyzedreactionsusingdeepeutecticsolventsbasedoncholinechlorid AT bareabruno towardsabetterunderstandingofhowtoimprovelipasecatalyzedreactionsusingdeepeutecticsolventsbasedoncholinechlorid AT villeneuvepierre towardsabetterunderstandingofhowtoimprovelipasecatalyzedreactionsusingdeepeutecticsolventsbasedoncholinechlorid |
_version_ |
1792498566966542336 |