The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine Production, and allostimulatory capacity of dendritic cells
Trypanosoma brucei gambiense, a parasitic protozoan belonging to kinetoplastids, is the main etiological agent of human African trypanosomiasis (HAT), or sleeping sickness. One major characteristic of this disease is the dysregulation of the host immune system. The present study demonstrates that the secretome (excreted-secreted proteins) of T. b. gambiense impairs the lipopolysaccharide (LPS)-induced maturation of murine dendritic cells (DCs). The upregulation of major histocompatibility complex class II, CD40, CD80, and CD86 molecules, as well as the secretion of cytokines such as tumor necrosis factor alpha, interleukin-10 (IL-10), and IL-6, which are normally released at high levels by LPS-stimulated DCs, is significantly reduced when these cells are cultured in the presence of the T. b. gambiense secretome. Moreover, the inhibition of DC maturation results in the loss of their allostimulatory capacity, leading to a dramatic decrease in Th1/Th2 cytokine production by cocultured lymphocytes. These results provide new insights into a novel efficient immunosuppressive mechanism directly involving the alteration of DC function which might be used by T. b. gambiense to interfere with the host immune responses in HAT and promote the infectious process.
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Subjects: | L72 - Organismes nuisibles des animaux, L73 - Maladies des animaux, |
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dig-cirad-fr-5716422022-03-30T15:04:44Z http://agritrop.cirad.fr/571642/ http://agritrop.cirad.fr/571642/ The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine Production, and allostimulatory capacity of dendritic cells. Garzon Edwin, Holzmuller Philippe, Bras-Gonçalves Rachel, Vincendeau Philippe, Cuny Gérard, Lemesre Jean-Loup, Geiger Anne. 2013. Infection and Immunity, 81 (9) : 3300-3308.https://doi.org/10.1128/IAI.00125-13 <https://doi.org/10.1128/IAI.00125-13> Researchers The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine Production, and allostimulatory capacity of dendritic cells Garzon, Edwin Holzmuller, Philippe Bras-Gonçalves, Rachel Vincendeau, Philippe Cuny, Gérard Lemesre, Jean-Loup Geiger, Anne eng 2013 Infection and Immunity L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Trypanosoma brucei gambiense, a parasitic protozoan belonging to kinetoplastids, is the main etiological agent of human African trypanosomiasis (HAT), or sleeping sickness. One major characteristic of this disease is the dysregulation of the host immune system. The present study demonstrates that the secretome (excreted-secreted proteins) of T. b. gambiense impairs the lipopolysaccharide (LPS)-induced maturation of murine dendritic cells (DCs). The upregulation of major histocompatibility complex class II, CD40, CD80, and CD86 molecules, as well as the secretion of cytokines such as tumor necrosis factor alpha, interleukin-10 (IL-10), and IL-6, which are normally released at high levels by LPS-stimulated DCs, is significantly reduced when these cells are cultured in the presence of the T. b. gambiense secretome. Moreover, the inhibition of DC maturation results in the loss of their allostimulatory capacity, leading to a dramatic decrease in Th1/Th2 cytokine production by cocultured lymphocytes. These results provide new insights into a novel efficient immunosuppressive mechanism directly involving the alteration of DC function which might be used by T. b. gambiense to interfere with the host immune responses in HAT and promote the infectious process. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/571642/1/document_571642.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1128/IAI.00125-13 10.1128/IAI.00125-13 info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.00125-13 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1128/IAI.00125-13 |
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L72 - Organismes nuisibles des animaux L73 - Maladies des animaux L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Garzon, Edwin Holzmuller, Philippe Bras-Gonçalves, Rachel Vincendeau, Philippe Cuny, Gérard Lemesre, Jean-Loup Geiger, Anne The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine Production, and allostimulatory capacity of dendritic cells |
description |
Trypanosoma brucei gambiense, a parasitic protozoan belonging to kinetoplastids, is the main etiological agent of human African trypanosomiasis (HAT), or sleeping sickness. One major characteristic of this disease is the dysregulation of the host immune system. The present study demonstrates that the secretome (excreted-secreted proteins) of T. b. gambiense impairs the lipopolysaccharide (LPS)-induced maturation of murine dendritic cells (DCs). The upregulation of major histocompatibility complex class II, CD40, CD80, and CD86 molecules, as well as the secretion of cytokines such as tumor necrosis factor alpha, interleukin-10 (IL-10), and IL-6, which are normally released at high levels by LPS-stimulated DCs, is significantly reduced when these cells are cultured in the presence of the T. b. gambiense secretome. Moreover, the inhibition of DC maturation results in the loss of their allostimulatory capacity, leading to a dramatic decrease in Th1/Th2 cytokine production by cocultured lymphocytes. These results provide new insights into a novel efficient immunosuppressive mechanism directly involving the alteration of DC function which might be used by T. b. gambiense to interfere with the host immune responses in HAT and promote the infectious process. |
format |
article |
topic_facet |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux |
author |
Garzon, Edwin Holzmuller, Philippe Bras-Gonçalves, Rachel Vincendeau, Philippe Cuny, Gérard Lemesre, Jean-Loup Geiger, Anne |
author_facet |
Garzon, Edwin Holzmuller, Philippe Bras-Gonçalves, Rachel Vincendeau, Philippe Cuny, Gérard Lemesre, Jean-Loup Geiger, Anne |
author_sort |
Garzon, Edwin |
title |
The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine Production, and allostimulatory capacity of dendritic cells |
title_short |
The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine Production, and allostimulatory capacity of dendritic cells |
title_full |
The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine Production, and allostimulatory capacity of dendritic cells |
title_fullStr |
The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine Production, and allostimulatory capacity of dendritic cells |
title_full_unstemmed |
The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine Production, and allostimulatory capacity of dendritic cells |
title_sort |
trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine production, and allostimulatory capacity of dendritic cells |
url |
http://agritrop.cirad.fr/571642/ http://agritrop.cirad.fr/571642/1/document_571642.pdf |
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