The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness
Natural rubber is harvested by tapping Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg. Harvesting stress can lead to tapping panel dryness (TPD). MicroRNAs (miRNAs) are induced by abiotic stress and regulate gene expression by targeting the cleavage or translational inhibition of target messenger RNAs. This study set out to sequence miRNAs expressed in latex cells and to identify TPD-related putative targets. Deep sequencing of small RNAs was carried out on latex from trees affected by TPD using Solexa technology. The most abundant small RNA class size was 21 nucleotides for TPD trees compared with 24 nucleotides in healthy trees. By combining the LeARN pipeline, data from the Plant MicroRNA database and Hevea EST sequences, we identified 19 additional conserved and four putative species-specific miRNA families not found in previous studies on rubber. The relative transcript abundance of the Hbpre-MIR159b gene increased with TPD. This study revealed a small RNA-specific signature of TPD-affected trees. Both RNA degradation and a shift in miRNA biogenesis are suggested to explain the general decline in small RNAs and, particularly, in miRNAs.
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Subjects: | F30 - Génétique et amélioration des plantes, F01 - Culture des plantes, F62 - Physiologie végétale - Croissance et développement, Hevea brasiliensis, expression des gènes, régulation physiologique, arn, séquence nucléotidique, latex, stress, récolte, oxydation, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_32719, http://aims.fao.org/aos/agrovoc/c_6618, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_4214, http://aims.fao.org/aos/agrovoc/c_7452, http://aims.fao.org/aos/agrovoc/c_3500, http://aims.fao.org/aos/agrovoc/c_5472, |
Online Access: | http://agritrop.cirad.fr/571551/ http://agritrop.cirad.fr/571551/1/document_571551.pdf |
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dig-cirad-fr-5715512024-01-28T21:45:34Z http://agritrop.cirad.fr/571551/ http://agritrop.cirad.fr/571551/ The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness. Gebelin Virginie, Leclercq Julie, Kuswanhadi, Argout Xavier, Chaidamsari Tetty, Hu Songnian, Tang Chaorong, Sarah Gautier, Yang Meng, Montoro Pascal. 2013. Tree Physiology, 33 (10) : 1084-1098.https://doi.org/10.1093/treephys/tpt076 <https://doi.org/10.1093/treephys/tpt076> The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness Gebelin, Virginie Leclercq, Julie Kuswanhadi, Argout, Xavier Chaidamsari, Tetty Hu, Songnian Tang, Chaorong Sarah, Gautier Yang, Meng Montoro, Pascal eng 2013 Tree Physiology F30 - Génétique et amélioration des plantes F01 - Culture des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis expression des gènes régulation physiologique arn séquence nucléotidique latex stress récolte oxydation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_3500 http://aims.fao.org/aos/agrovoc/c_5472 Natural rubber is harvested by tapping Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg. Harvesting stress can lead to tapping panel dryness (TPD). MicroRNAs (miRNAs) are induced by abiotic stress and regulate gene expression by targeting the cleavage or translational inhibition of target messenger RNAs. This study set out to sequence miRNAs expressed in latex cells and to identify TPD-related putative targets. Deep sequencing of small RNAs was carried out on latex from trees affected by TPD using Solexa technology. The most abundant small RNA class size was 21 nucleotides for TPD trees compared with 24 nucleotides in healthy trees. By combining the LeARN pipeline, data from the Plant MicroRNA database and Hevea EST sequences, we identified 19 additional conserved and four putative species-specific miRNA families not found in previous studies on rubber. The relative transcript abundance of the Hbpre-MIR159b gene increased with TPD. This study revealed a small RNA-specific signature of TPD-affected trees. Both RNA degradation and a shift in miRNA biogenesis are suggested to explain the general decline in small RNAs and, particularly, in miRNAs. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/571551/1/document_571551.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1093/treephys/tpt076 10.1093/treephys/tpt076 info:eu-repo/semantics/altIdentifier/doi/10.1093/treephys/tpt076 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1093/treephys/tpt076 |
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F30 - Génétique et amélioration des plantes F01 - Culture des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis expression des gènes régulation physiologique arn séquence nucléotidique latex stress récolte oxydation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_3500 http://aims.fao.org/aos/agrovoc/c_5472 F30 - Génétique et amélioration des plantes F01 - Culture des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis expression des gènes régulation physiologique arn séquence nucléotidique latex stress récolte oxydation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_3500 http://aims.fao.org/aos/agrovoc/c_5472 |
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F30 - Génétique et amélioration des plantes F01 - Culture des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis expression des gènes régulation physiologique arn séquence nucléotidique latex stress récolte oxydation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_3500 http://aims.fao.org/aos/agrovoc/c_5472 F30 - Génétique et amélioration des plantes F01 - Culture des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis expression des gènes régulation physiologique arn séquence nucléotidique latex stress récolte oxydation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_3500 http://aims.fao.org/aos/agrovoc/c_5472 Gebelin, Virginie Leclercq, Julie Kuswanhadi, Argout, Xavier Chaidamsari, Tetty Hu, Songnian Tang, Chaorong Sarah, Gautier Yang, Meng Montoro, Pascal The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness |
description |
Natural rubber is harvested by tapping Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg. Harvesting stress can lead to tapping panel dryness (TPD). MicroRNAs (miRNAs) are induced by abiotic stress and regulate gene expression by targeting the cleavage or translational inhibition of target messenger RNAs. This study set out to sequence miRNAs expressed in latex cells and to identify TPD-related putative targets. Deep sequencing of small RNAs was carried out on latex from trees affected by TPD using Solexa technology. The most abundant small RNA class size was 21 nucleotides for TPD trees compared with 24 nucleotides in healthy trees. By combining the LeARN pipeline, data from the Plant MicroRNA database and Hevea EST sequences, we identified 19 additional conserved and four putative species-specific miRNA families not found in previous studies on rubber. The relative transcript abundance of the Hbpre-MIR159b gene increased with TPD. This study revealed a small RNA-specific signature of TPD-affected trees. Both RNA degradation and a shift in miRNA biogenesis are suggested to explain the general decline in small RNAs and, particularly, in miRNAs. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F01 - Culture des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis expression des gènes régulation physiologique arn séquence nucléotidique latex stress récolte oxydation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_32719 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_3500 http://aims.fao.org/aos/agrovoc/c_5472 |
author |
Gebelin, Virginie Leclercq, Julie Kuswanhadi, Argout, Xavier Chaidamsari, Tetty Hu, Songnian Tang, Chaorong Sarah, Gautier Yang, Meng Montoro, Pascal |
author_facet |
Gebelin, Virginie Leclercq, Julie Kuswanhadi, Argout, Xavier Chaidamsari, Tetty Hu, Songnian Tang, Chaorong Sarah, Gautier Yang, Meng Montoro, Pascal |
author_sort |
Gebelin, Virginie |
title |
The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness |
title_short |
The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness |
title_full |
The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness |
title_fullStr |
The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness |
title_full_unstemmed |
The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness |
title_sort |
small rna profile in latex from hevea brasiliensis trees is affected by tapping panel dryness |
url |
http://agritrop.cirad.fr/571551/ http://agritrop.cirad.fr/571551/1/document_571551.pdf |
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