A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): Cloning, characterization and relation to postharvest chilling stress resistance
A full-length cDNA encoding a putative aspartic acid protease (AcAP1) was isolated for the first time from the flesh of pineapple (Ananas comosus) fruit. The deduced sequence of AcAP1 showed all the com-mon features of a typical plant aspartic protease phytepsin precursor. Analysis of AcAP1 gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development revealed opposite trends. The resistant variety showed an up-regulation of AcAP1 pre-cursor gene expression whereas the susceptible showed a down-regulation in response to postharvest chilling treatment. The same trend was observed regarding specific AP enzyme activity in both varieties. Taken together our results support the involvement of AcAP1 in postharvest chilling stress resistance in pineapple fruits.
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dig-cirad-fr-5712382022-03-30T15:05:25Z http://agritrop.cirad.fr/571238/ http://agritrop.cirad.fr/571238/ A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): Cloning, characterization and relation to postharvest chilling stress resistance. Raimbault Astrid-Kim, Zuily Fodil Yasmine, Soler Alain, Cruz de Carvalho Maria. 2013. Journal of Plant Physiology, 170 (17) : 1536-1540.https://doi.org/10.1016/j.jplph.2013.06.007 <https://doi.org/10.1016/j.jplph.2013.06.007> Researchers A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): Cloning, characterization and relation to postharvest chilling stress resistance Raimbault, Astrid-Kim Zuily Fodil, Yasmine Soler, Alain Cruz de Carvalho, Maria eng 2013 Journal of Plant Physiology F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale H50 - Troubles divers des plantes A full-length cDNA encoding a putative aspartic acid protease (AcAP1) was isolated for the first time from the flesh of pineapple (Ananas comosus) fruit. The deduced sequence of AcAP1 showed all the com-mon features of a typical plant aspartic protease phytepsin precursor. Analysis of AcAP1 gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development revealed opposite trends. The resistant variety showed an up-regulation of AcAP1 pre-cursor gene expression whereas the susceptible showed a down-regulation in response to postharvest chilling treatment. The same trend was observed regarding specific AP enzyme activity in both varieties. Taken together our results support the involvement of AcAP1 in postharvest chilling stress resistance in pineapple fruits. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/571238/1/document_571238.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.jplph.2013.06.007 10.1016/j.jplph.2013.06.007 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2013.06.007 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.jplph.2013.06.007 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale H50 - Troubles divers des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale H50 - Troubles divers des plantes |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale H50 - Troubles divers des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale H50 - Troubles divers des plantes Raimbault, Astrid-Kim Zuily Fodil, Yasmine Soler, Alain Cruz de Carvalho, Maria A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): Cloning, characterization and relation to postharvest chilling stress resistance |
| description |
A full-length cDNA encoding a putative aspartic acid protease (AcAP1) was isolated for the first time from the flesh of pineapple (Ananas comosus) fruit. The deduced sequence of AcAP1 showed all the com-mon features of a typical plant aspartic protease phytepsin precursor. Analysis of AcAP1 gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development revealed opposite trends. The resistant variety showed an up-regulation of AcAP1 pre-cursor gene expression whereas the susceptible showed a down-regulation in response to postharvest chilling treatment. The same trend was observed regarding specific AP enzyme activity in both varieties. Taken together our results support the involvement of AcAP1 in postharvest chilling stress resistance in pineapple fruits. |
| format |
article |
| topic_facet |
F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale H50 - Troubles divers des plantes |
| author |
Raimbault, Astrid-Kim Zuily Fodil, Yasmine Soler, Alain Cruz de Carvalho, Maria |
| author_facet |
Raimbault, Astrid-Kim Zuily Fodil, Yasmine Soler, Alain Cruz de Carvalho, Maria |
| author_sort |
Raimbault, Astrid-Kim |
| title |
A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): Cloning, characterization and relation to postharvest chilling stress resistance |
| title_short |
A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): Cloning, characterization and relation to postharvest chilling stress resistance |
| title_full |
A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): Cloning, characterization and relation to postharvest chilling stress resistance |
| title_fullStr |
A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): Cloning, characterization and relation to postharvest chilling stress resistance |
| title_full_unstemmed |
A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): Cloning, characterization and relation to postharvest chilling stress resistance |
| title_sort |
novel aspartic acid protease gene from pineapple fruit (ananas comosus): cloning, characterization and relation to postharvest chilling stress resistance |
| url |
http://agritrop.cirad.fr/571238/ http://agritrop.cirad.fr/571238/1/document_571238.pdf |
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