Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes
Genetic analysis of phenotypical traits and marker-trait association in polyploid species is generally considered as a challenge. In the present work, different approaches were combined taking advantage of the particular genetic structures of 2n gametes resulting from second division restitution (SDR) to map a genome region linked to Alternaria brown spot (ABS) resistance in triploid citrus progeny. ABS in citrus is a serious disease caused by the tangerine pathotype of the fungus Alternaria alternata. This pathogen produces ACT-toxin, which induces necrotic lesions on fruit and young leaves, defoliation and fruit drop in susceptible genotypes. It is a strong concern for triploid breeding programs aiming to produce seedless mandarin cultivars. The monolocus dominant inheritance of susceptibility, proposed on the basis of diploid population studies, was corroborated in triploid progeny. Bulk segregant analysis coupled with genome scan using a large set of genetically mapped SNP markers and targeted genetic mapping by half tetrad analysis, using SSR and SNP markers, allowed locating a 3.3 Mb genomic region linked to ABS resistance near the centromere of chromosome III. Clusters of resistance genes were identified by gene ontology analysis of this genomic region. Some of these genes are good candidates to control the dominant susceptibility to the ACTtoxin. SSR and SNP markers were developed for efficient early marker-assisted selection of ABS resistant hybrids.
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Subjects: | H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes, Citrus, Citrus paradisi, mandarine, Alternaria alternata, résistance aux maladies, résistance génétique, carte génétique, marqueur génétique, triploïdie, Citrus clementina, http://aims.fao.org/aos/agrovoc/c_1637, http://aims.fao.org/aos/agrovoc/c_1645, http://aims.fao.org/aos/agrovoc/c_4568, http://aims.fao.org/aos/agrovoc/c_33593, http://aims.fao.org/aos/agrovoc/c_2328, http://aims.fao.org/aos/agrovoc/c_35130, http://aims.fao.org/aos/agrovoc/c_24002, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_7939, http://aims.fao.org/aos/agrovoc/c_6ce991dd, |
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dig-cirad-fr-5708062024-01-28T21:40:37Z http://agritrop.cirad.fr/570806/ http://agritrop.cirad.fr/570806/ Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes. Cuenca José, Aleza Pablo, Vicent Antonio, Brunel Dominique, Ollitrault Patrick, Navarro Luis. 2013. PloS One, 8 (10):e76755, 18 p.https://doi.org/10.1371/journal.pone.0076755 <https://doi.org/10.1371/journal.pone.0076755> Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes Cuenca, José Aleza, Pablo Vicent, Antonio Brunel, Dominique Ollitrault, Patrick Navarro, Luis eng 2013 PloS One H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Citrus Citrus paradisi mandarine Alternaria alternata résistance aux maladies résistance génétique carte génétique marqueur génétique triploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_4568 http://aims.fao.org/aos/agrovoc/c_33593 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7939 http://aims.fao.org/aos/agrovoc/c_6ce991dd Genetic analysis of phenotypical traits and marker-trait association in polyploid species is generally considered as a challenge. In the present work, different approaches were combined taking advantage of the particular genetic structures of 2n gametes resulting from second division restitution (SDR) to map a genome region linked to Alternaria brown spot (ABS) resistance in triploid citrus progeny. ABS in citrus is a serious disease caused by the tangerine pathotype of the fungus Alternaria alternata. This pathogen produces ACT-toxin, which induces necrotic lesions on fruit and young leaves, defoliation and fruit drop in susceptible genotypes. It is a strong concern for triploid breeding programs aiming to produce seedless mandarin cultivars. The monolocus dominant inheritance of susceptibility, proposed on the basis of diploid population studies, was corroborated in triploid progeny. Bulk segregant analysis coupled with genome scan using a large set of genetically mapped SNP markers and targeted genetic mapping by half tetrad analysis, using SSR and SNP markers, allowed locating a 3.3 Mb genomic region linked to ABS resistance near the centromere of chromosome III. Clusters of resistance genes were identified by gene ontology analysis of this genomic region. Some of these genes are good candidates to control the dominant susceptibility to the ACTtoxin. SSR and SNP markers were developed for efficient early marker-assisted selection of ABS resistant hybrids. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/570806/1/document_570806.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1371/journal.pone.0076755 10.1371/journal.pone.0076755 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0076755 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1371/journal.pone.0076755 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Citrus Citrus paradisi mandarine Alternaria alternata résistance aux maladies résistance génétique carte génétique marqueur génétique triploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_4568 http://aims.fao.org/aos/agrovoc/c_33593 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7939 http://aims.fao.org/aos/agrovoc/c_6ce991dd H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Citrus Citrus paradisi mandarine Alternaria alternata résistance aux maladies résistance génétique carte génétique marqueur génétique triploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_4568 http://aims.fao.org/aos/agrovoc/c_33593 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7939 http://aims.fao.org/aos/agrovoc/c_6ce991dd |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Citrus Citrus paradisi mandarine Alternaria alternata résistance aux maladies résistance génétique carte génétique marqueur génétique triploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_4568 http://aims.fao.org/aos/agrovoc/c_33593 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7939 http://aims.fao.org/aos/agrovoc/c_6ce991dd H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Citrus Citrus paradisi mandarine Alternaria alternata résistance aux maladies résistance génétique carte génétique marqueur génétique triploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_4568 http://aims.fao.org/aos/agrovoc/c_33593 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7939 http://aims.fao.org/aos/agrovoc/c_6ce991dd Cuenca, José Aleza, Pablo Vicent, Antonio Brunel, Dominique Ollitrault, Patrick Navarro, Luis Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes |
description |
Genetic analysis of phenotypical traits and marker-trait association in polyploid species is generally considered as a challenge. In the present work, different approaches were combined taking advantage of the particular genetic structures of 2n gametes resulting from second division restitution (SDR) to map a genome region linked to Alternaria brown spot (ABS) resistance in triploid citrus progeny. ABS in citrus is a serious disease caused by the tangerine pathotype of the fungus Alternaria alternata. This pathogen produces ACT-toxin, which induces necrotic lesions on fruit and young leaves, defoliation and fruit drop in susceptible genotypes. It is a strong concern for triploid breeding programs aiming to produce seedless mandarin cultivars. The monolocus dominant inheritance of susceptibility, proposed on the basis of diploid population studies, was corroborated in triploid progeny. Bulk segregant analysis coupled with genome scan using a large set of genetically mapped SNP markers and targeted genetic mapping by half tetrad analysis, using SSR and SNP markers, allowed locating a 3.3 Mb genomic region linked to ABS resistance near the centromere of chromosome III. Clusters of resistance genes were identified by gene ontology analysis of this genomic region. Some of these genes are good candidates to control the dominant susceptibility to the ACTtoxin. SSR and SNP markers were developed for efficient early marker-assisted selection of ABS resistant hybrids. |
format |
article |
topic_facet |
H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Citrus Citrus paradisi mandarine Alternaria alternata résistance aux maladies résistance génétique carte génétique marqueur génétique triploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_4568 http://aims.fao.org/aos/agrovoc/c_33593 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7939 http://aims.fao.org/aos/agrovoc/c_6ce991dd |
author |
Cuenca, José Aleza, Pablo Vicent, Antonio Brunel, Dominique Ollitrault, Patrick Navarro, Luis |
author_facet |
Cuenca, José Aleza, Pablo Vicent, Antonio Brunel, Dominique Ollitrault, Patrick Navarro, Luis |
author_sort |
Cuenca, José |
title |
Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes |
title_short |
Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes |
title_full |
Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes |
title_fullStr |
Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes |
title_full_unstemmed |
Genetically based location from triploid populations and gene ontology of a 3.3-Mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes |
title_sort |
genetically based location from triploid populations and gene ontology of a 3.3-mb genome region linked to alternaria brown spot resistance in citrus reveal clusters of resistance genes |
url |
http://agritrop.cirad.fr/570806/ http://agritrop.cirad.fr/570806/1/document_570806.pdf |
work_keys_str_mv |
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_version_ |
1792498536046133248 |