Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives
Since the work of Orlando Lopez-Baez in 1993, great improvements on cacao somatic embryogenesis from sporophytic tissues have been obtained. Recently, Li et al. (1998) developped a new protocole. It is now possible to produce somatic plantlets from different genotypes. After the definition of somatic embryogenesis, a bibliographic synthesis will be presented, focusing on the limitations that still exist. Cacao somatic embryogenesis has been developed at Cirad for several years. Li's protocole has been tested on many different genotypes. The efficiency of each step of the process has been determined. Histological studies were initiated in order to determine the origin of the somatic embryos. They were demonstrated to be of multicellular origin. This fact has implications for mass production and genetic transformation. RITA, a temporary immersion system developped at CIRAD, has been tested on cacao somatic embryogenesis because this system was used to improve several tissue culture processes on different plants. It significantly improved embryo production and growth. Finally, the use of cacao somatic embryogenesis in breeding programmes will be discussed, as well as the studies that need to be continued in this field. (Texte intégral)
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dig-cirad-fr-5707202015-09-09T12:07:44Z http://agritrop.cirad.fr/570720/ http://agritrop.cirad.fr/570720/ Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives. Alemanno Laurence, Ferrière Nicole. 1999. In : CUBACAFE ' 99. Simposio Internacional de Café y Cacao, programa, conferencias y resumenes, 25-27 de Noviembre de 1999, Santiago de Cuba. ECICC. Tercer Frente : Estacion Central de Investigaciones de Café y Cacao, Résumé, 37. Simposio Internacional de Café y Cacao, Santiago de Cuba, Cuba, 25 Novembre 1999/27 Novembre 1999. Researchers Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives Alemanno, Laurence Ferrière, Nicole eng 1999 Estacion Central de Investigaciones de Café y Cacao CUBACAFE ' 99. Simposio Internacional de Café y Cacao, programa, conferencias y resumenes, 25-27 de Noviembre de 1999, Santiago de Cuba F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes Since the work of Orlando Lopez-Baez in 1993, great improvements on cacao somatic embryogenesis from sporophytic tissues have been obtained. Recently, Li et al. (1998) developped a new protocole. It is now possible to produce somatic plantlets from different genotypes. After the definition of somatic embryogenesis, a bibliographic synthesis will be presented, focusing on the limitations that still exist. Cacao somatic embryogenesis has been developed at Cirad for several years. Li's protocole has been tested on many different genotypes. The efficiency of each step of the process has been determined. Histological studies were initiated in order to determine the origin of the somatic embryos. They were demonstrated to be of multicellular origin. This fact has implications for mass production and genetic transformation. RITA, a temporary immersion system developped at CIRAD, has been tested on cacao somatic embryogenesis because this system was used to improve several tissue culture processes on different plants. It significantly improved embryo production and growth. Finally, the use of cacao somatic embryogenesis in breeding programmes will be discussed, as well as the studies that need to be continued in this field. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=216626 |
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F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes Alemanno, Laurence Ferrière, Nicole Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives |
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Since the work of Orlando Lopez-Baez in 1993, great improvements on cacao somatic embryogenesis from sporophytic tissues have been obtained. Recently, Li et al. (1998) developped a new protocole. It is now possible to produce somatic plantlets from different genotypes. After the definition of somatic embryogenesis, a bibliographic synthesis will be presented, focusing on the limitations that still exist. Cacao somatic embryogenesis has been developed at Cirad for several years. Li's protocole has been tested on many different genotypes. The efficiency of each step of the process has been determined. Histological studies were initiated in order to determine the origin of the somatic embryos. They were demonstrated to be of multicellular origin. This fact has implications for mass production and genetic transformation. RITA, a temporary immersion system developped at CIRAD, has been tested on cacao somatic embryogenesis because this system was used to improve several tissue culture processes on different plants. It significantly improved embryo production and growth. Finally, the use of cacao somatic embryogenesis in breeding programmes will be discussed, as well as the studies that need to be continued in this field. (Texte intégral) |
format |
conference_item |
topic_facet |
F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes |
author |
Alemanno, Laurence Ferrière, Nicole |
author_facet |
Alemanno, Laurence Ferrière, Nicole |
author_sort |
Alemanno, Laurence |
title |
Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives |
title_short |
Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives |
title_full |
Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives |
title_fullStr |
Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives |
title_full_unstemmed |
Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives |
title_sort |
cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives |
publisher |
Estacion Central de Investigaciones de Café y Cacao |
url |
http://agritrop.cirad.fr/570720/ |
work_keys_str_mv |
AT alemannolaurence cacaosomaticembryogenesisasatoolinbreedingprogramsstatementsandperspectives AT ferrierenicole cacaosomaticembryogenesisasatoolinbreedingprogramsstatementsandperspectives |
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1758023916273532928 |