Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane
Several EZ-Tn5 insertions in gene locus XALc_0557 (OmpA1) of the sugarcane leaf scald pathogen Xanthomonas albilineans XaFL07-1 were previously found to strongly affect pathogenicity and endophytic stalk colonization. XALc_0557 has a predicted OmpA N-terminal outer membrane channel (OMC) domain and an OmpA C-like domain. Further analysis of mutant M468, with an EZ-Tn5 insertion in the upstream OMC domain coding region, revealed impaired epiphytic and endophytic leaf survival, impaired resistance to sodium dodecyl sulfate (SDS), structural defects in the outer membrane (OM), and hyperproduction of OM vesicles. Cloned full-length XALc_0557 complemented M468 for all phenotypes tested, including pathogenicity, resistance to SDS, and ability to survive both endophytically and epiphytically. Another construct, pCT47.3, which expressed only the C-like domain of XALc_0557, restored resistance to SDS in M468 but failed to complement any other mutant phenotype, indicating that the C-like domain functioned independently of the OMC domain to help maintain OM integrity. pCT47.3 also complemented pathogenicity, resistance to SDS, and stalk colonization in mutant M1152, which carries an EZ-Tn5 insert in the C-like coding region, indicating that both predicted domains are modular and necessary but neither is sufficient for X. albilineans pathogenicity, endophytic survival in, and epiphytic survival on sugarcane.
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Subjects: | H20 - Maladies des plantes, Xanthomonas albilineans, Saccharum officinarum, pouvoir pathogène, mutant, protéine microbienne, Escherichia coli, génie génétique, résistance aux maladies, http://aims.fao.org/aos/agrovoc/c_27422, http://aims.fao.org/aos/agrovoc/c_6727, http://aims.fao.org/aos/agrovoc/c_5629, http://aims.fao.org/aos/agrovoc/c_5013, http://aims.fao.org/aos/agrovoc/c_12537, http://aims.fao.org/aos/agrovoc/c_33700, http://aims.fao.org/aos/agrovoc/c_15974, http://aims.fao.org/aos/agrovoc/c_2328, |
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dig-cirad-fr-5704502024-01-28T21:37:22Z http://agritrop.cirad.fr/570450/ http://agritrop.cirad.fr/570450/ Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane. Fleites Laura, Mensi Imène, Gargani Daniel, Zhang Shujian, Rott Philippe, Gabriel Dean W.. 2013. Molecular Plant-Microbe Interactions, 26 (10) : 1200-1210.https://doi.org/10.1094/MPMI-01-13-0002-R <https://doi.org/10.1094/MPMI-01-13-0002-R> Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane Fleites, Laura Mensi, Imène Gargani, Daniel Zhang, Shujian Rott, Philippe Gabriel, Dean W. eng 2013 Molecular Plant-Microbe Interactions H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum pouvoir pathogène mutant protéine microbienne Escherichia coli génie génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_12537 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2328 Several EZ-Tn5 insertions in gene locus XALc_0557 (OmpA1) of the sugarcane leaf scald pathogen Xanthomonas albilineans XaFL07-1 were previously found to strongly affect pathogenicity and endophytic stalk colonization. XALc_0557 has a predicted OmpA N-terminal outer membrane channel (OMC) domain and an OmpA C-like domain. Further analysis of mutant M468, with an EZ-Tn5 insertion in the upstream OMC domain coding region, revealed impaired epiphytic and endophytic leaf survival, impaired resistance to sodium dodecyl sulfate (SDS), structural defects in the outer membrane (OM), and hyperproduction of OM vesicles. Cloned full-length XALc_0557 complemented M468 for all phenotypes tested, including pathogenicity, resistance to SDS, and ability to survive both endophytically and epiphytically. Another construct, pCT47.3, which expressed only the C-like domain of XALc_0557, restored resistance to SDS in M468 but failed to complement any other mutant phenotype, indicating that the C-like domain functioned independently of the OMC domain to help maintain OM integrity. pCT47.3 also complemented pathogenicity, resistance to SDS, and stalk colonization in mutant M1152, which carries an EZ-Tn5 insert in the C-like coding region, indicating that both predicted domains are modular and necessary but neither is sufficient for X. albilineans pathogenicity, endophytic survival in, and epiphytic survival on sugarcane. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/570450/1/document_570450.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1094/MPMI-01-13-0002-R 10.1094/MPMI-01-13-0002-R info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-01-13-0002-R info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1094/MPMI-01-13-0002-R |
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H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum pouvoir pathogène mutant protéine microbienne Escherichia coli génie génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_12537 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2328 H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum pouvoir pathogène mutant protéine microbienne Escherichia coli génie génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_12537 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2328 |
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H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum pouvoir pathogène mutant protéine microbienne Escherichia coli génie génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_12537 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2328 H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum pouvoir pathogène mutant protéine microbienne Escherichia coli génie génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_12537 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2328 Fleites, Laura Mensi, Imène Gargani, Daniel Zhang, Shujian Rott, Philippe Gabriel, Dean W. Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane |
description |
Several EZ-Tn5 insertions in gene locus XALc_0557 (OmpA1) of the sugarcane leaf scald pathogen Xanthomonas albilineans XaFL07-1 were previously found to strongly affect pathogenicity and endophytic stalk colonization. XALc_0557 has a predicted OmpA N-terminal outer membrane channel (OMC) domain and an OmpA C-like domain. Further analysis of mutant M468, with an EZ-Tn5 insertion in the upstream OMC domain coding region, revealed impaired epiphytic and endophytic leaf survival, impaired resistance to sodium dodecyl sulfate (SDS), structural defects in the outer membrane (OM), and hyperproduction of OM vesicles. Cloned full-length XALc_0557 complemented M468 for all phenotypes tested, including pathogenicity, resistance to SDS, and ability to survive both endophytically and epiphytically. Another construct, pCT47.3, which expressed only the C-like domain of XALc_0557, restored resistance to SDS in M468 but failed to complement any other mutant phenotype, indicating that the C-like domain functioned independently of the OMC domain to help maintain OM integrity. pCT47.3 also complemented pathogenicity, resistance to SDS, and stalk colonization in mutant M1152, which carries an EZ-Tn5 insert in the C-like coding region, indicating that both predicted domains are modular and necessary but neither is sufficient for X. albilineans pathogenicity, endophytic survival in, and epiphytic survival on sugarcane. |
format |
article |
topic_facet |
H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum pouvoir pathogène mutant protéine microbienne Escherichia coli génie génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_12537 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2328 |
author |
Fleites, Laura Mensi, Imène Gargani, Daniel Zhang, Shujian Rott, Philippe Gabriel, Dean W. |
author_facet |
Fleites, Laura Mensi, Imène Gargani, Daniel Zhang, Shujian Rott, Philippe Gabriel, Dean W. |
author_sort |
Fleites, Laura |
title |
Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane |
title_short |
Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane |
title_full |
Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane |
title_fullStr |
Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane |
title_full_unstemmed |
Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane |
title_sort |
xanthomonas albilineans ompa1 appears to be functionally modular and both the omc and c-like domains are necessary for leaf scald disease of sugarcane |
url |
http://agritrop.cirad.fr/570450/ http://agritrop.cirad.fr/570450/1/document_570450.pdf |
work_keys_str_mv |
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