Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects
In Coffea arabica L., the development of direct sowing of somatic embryos (SE) in planting substrate, with subsequent nursery production of plants, has promoted the industrialization of somatic embryogenesis. However, plant conversion rates are still low and require improvements to enhance the cost-effectiveness of commercial micropropagation. With the aim of improving plant regeneration from SE, we studied the morphological and histological criteria and water characteristics during germination and plant conversion of zygotic embryos (ZE) and SE. At the cotyledonary stage, SE produced in a 1 l RITA® temporary immersion bioreactor (area 55.8 cm2) were morphologically similar in size (2-3 mm) but abnormal as compared with mature ZE. Protein and starch reserve levels were extremely low throughout germination and conversion to plantlets, while the water status remained steady [water content (WC) from 76 to 87%, ? from ?0.37 to ?0.47 MPa, pressure potential from 0.69 to 0.24 MPa]. In ZE, spectacular hydration occurred during the first 3 weeks (WC from 37 to 75%; ? from ?6.24 to ?1.0 MPa). Cotyledons remained undifferentiated for 10 weeks after sowing. Conversely, after only 3 weeks under germination conditions in a RITA® bioreactor, spongy and palisade parenchyma and stomata formed in SE cotyledons. The ZE plant conversion was faster than that of SE (14 vs. 22 weeks) and more efficient (rates 96 vs. 55%), with much more substantial hypocotyl and cotyledon development. The use of a new 5 l MATIS® bioreactor (area 355 cm2), designed especially to favor embryo dispersion and light transmittance to SE, markedly improved the embryo-to-plantlet conversion rate (91%). These results highlight the morphological heterogeneity and lack of protein reserves in SE at the beginning of the germination phase and marked differences in water characteristics. However, they also reveal high phenotypic plasticity, leading to a highly efficient plantlet conversion rate due to better embryo dispersion and light transmittance in more horizontal bioreactors.
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F02 - Multiplication végétative des plantes F50 - Anatomie et morphologie des plantes F60 - Physiologie et biochimie végétale Coffea arabica régénération in vitro culture d'embryon embryon somatique embryon végétal Embryogénèse somatique germination hydratation protéine de réserve physiologie végétale anatomie végétale bioréacteur microscopie http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_27607 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_3247 http://aims.fao.org/aos/agrovoc/c_3712 http://aims.fao.org/aos/agrovoc/c_33936 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_5954 http://aims.fao.org/aos/agrovoc/c_27469 http://aims.fao.org/aos/agrovoc/c_4810 http://aims.fao.org/aos/agrovoc/c_5171 http://aims.fao.org/aos/agrovoc/c_1920 F02 - Multiplication végétative des plantes F50 - Anatomie et morphologie des plantes F60 - Physiologie et biochimie végétale Coffea arabica régénération in vitro culture d'embryon embryon somatique embryon végétal Embryogénèse somatique germination hydratation protéine de réserve physiologie végétale anatomie végétale bioréacteur microscopie http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_27607 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_3247 http://aims.fao.org/aos/agrovoc/c_3712 http://aims.fao.org/aos/agrovoc/c_33936 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_5954 http://aims.fao.org/aos/agrovoc/c_27469 http://aims.fao.org/aos/agrovoc/c_4810 http://aims.fao.org/aos/agrovoc/c_5171 http://aims.fao.org/aos/agrovoc/c_1920 |
spellingShingle |
F02 - Multiplication végétative des plantes F50 - Anatomie et morphologie des plantes F60 - Physiologie et biochimie végétale Coffea arabica régénération in vitro culture d'embryon embryon somatique embryon végétal Embryogénèse somatique germination hydratation protéine de réserve physiologie végétale anatomie végétale bioréacteur microscopie http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_27607 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_3247 http://aims.fao.org/aos/agrovoc/c_3712 http://aims.fao.org/aos/agrovoc/c_33936 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_5954 http://aims.fao.org/aos/agrovoc/c_27469 http://aims.fao.org/aos/agrovoc/c_4810 http://aims.fao.org/aos/agrovoc/c_5171 http://aims.fao.org/aos/agrovoc/c_1920 F02 - Multiplication végétative des plantes F50 - Anatomie et morphologie des plantes F60 - Physiologie et biochimie végétale Coffea arabica régénération in vitro culture d'embryon embryon somatique embryon végétal Embryogénèse somatique germination hydratation protéine de réserve physiologie végétale anatomie végétale bioréacteur microscopie http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_27607 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_3247 http://aims.fao.org/aos/agrovoc/c_3712 http://aims.fao.org/aos/agrovoc/c_33936 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_5954 http://aims.fao.org/aos/agrovoc/c_27469 http://aims.fao.org/aos/agrovoc/c_4810 http://aims.fao.org/aos/agrovoc/c_5171 http://aims.fao.org/aos/agrovoc/c_1920 Etienne, Hervé Bertrand, Benoît Georget, Frederic Lartaud, Marc Montes, Fabienne Dechamp, Eveline Verdeil, Jean-Luc Barry-Etienne, Dominique Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects |
description |
In Coffea arabica L., the development of direct sowing of somatic embryos (SE) in planting substrate, with subsequent nursery production of plants, has promoted the industrialization of somatic embryogenesis. However, plant conversion rates are still low and require improvements to enhance the cost-effectiveness of commercial micropropagation. With the aim of improving plant regeneration from SE, we studied the morphological and histological criteria and water characteristics during germination and plant conversion of zygotic embryos (ZE) and SE. At the cotyledonary stage, SE produced in a 1 l RITA® temporary immersion bioreactor (area 55.8 cm2) were morphologically similar in size (2-3 mm) but abnormal as compared with mature ZE. Protein and starch reserve levels were extremely low throughout germination and conversion to plantlets, while the water status remained steady [water content (WC) from 76 to 87%, ? from ?0.37 to ?0.47 MPa, pressure potential from 0.69 to 0.24 MPa]. In ZE, spectacular hydration occurred during the first 3 weeks (WC from 37 to 75%; ? from ?6.24 to ?1.0 MPa). Cotyledons remained undifferentiated for 10 weeks after sowing. Conversely, after only 3 weeks under germination conditions in a RITA® bioreactor, spongy and palisade parenchyma and stomata formed in SE cotyledons. The ZE plant conversion was faster than that of SE (14 vs. 22 weeks) and more efficient (rates 96 vs. 55%), with much more substantial hypocotyl and cotyledon development. The use of a new 5 l MATIS® bioreactor (area 355 cm2), designed especially to favor embryo dispersion and light transmittance to SE, markedly improved the embryo-to-plantlet conversion rate (91%). These results highlight the morphological heterogeneity and lack of protein reserves in SE at the beginning of the germination phase and marked differences in water characteristics. However, they also reveal high phenotypic plasticity, leading to a highly efficient plantlet conversion rate due to better embryo dispersion and light transmittance in more horizontal bioreactors. |
format |
article |
topic_facet |
F02 - Multiplication végétative des plantes F50 - Anatomie et morphologie des plantes F60 - Physiologie et biochimie végétale Coffea arabica régénération in vitro culture d'embryon embryon somatique embryon végétal Embryogénèse somatique germination hydratation protéine de réserve physiologie végétale anatomie végétale bioréacteur microscopie http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_27607 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_3247 http://aims.fao.org/aos/agrovoc/c_3712 http://aims.fao.org/aos/agrovoc/c_33936 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_5954 http://aims.fao.org/aos/agrovoc/c_27469 http://aims.fao.org/aos/agrovoc/c_4810 http://aims.fao.org/aos/agrovoc/c_5171 http://aims.fao.org/aos/agrovoc/c_1920 |
author |
Etienne, Hervé Bertrand, Benoît Georget, Frederic Lartaud, Marc Montes, Fabienne Dechamp, Eveline Verdeil, Jean-Luc Barry-Etienne, Dominique |
author_facet |
Etienne, Hervé Bertrand, Benoît Georget, Frederic Lartaud, Marc Montes, Fabienne Dechamp, Eveline Verdeil, Jean-Luc Barry-Etienne, Dominique |
author_sort |
Etienne, Hervé |
title |
Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects |
title_short |
Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects |
title_full |
Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects |
title_fullStr |
Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects |
title_full_unstemmed |
Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects |
title_sort |
development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects |
url |
http://agritrop.cirad.fr/569804/ http://agritrop.cirad.fr/569804/1/document_569804.pdf |
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dig-cirad-fr-5698042024-01-28T21:31:58Z http://agritrop.cirad.fr/569804/ http://agritrop.cirad.fr/569804/ Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects. Etienne Hervé, Bertrand Benoît, Georget Frederic, Lartaud Marc, Montes Fabienne, Dechamp Eveline, Verdeil Jean-Luc, Barry-Etienne Dominique. 2013. Tree Physiology, 33 (6) : 640-653.https://doi.org/10.1093/treephys/tpt034 <https://doi.org/10.1093/treephys/tpt034> Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects Etienne, Hervé Bertrand, Benoît Georget, Frederic Lartaud, Marc Montes, Fabienne Dechamp, Eveline Verdeil, Jean-Luc Barry-Etienne, Dominique eng 2013 Tree Physiology F02 - Multiplication végétative des plantes F50 - Anatomie et morphologie des plantes F60 - Physiologie et biochimie végétale Coffea arabica régénération in vitro culture d'embryon embryon somatique embryon végétal Embryogénèse somatique germination hydratation protéine de réserve physiologie végétale anatomie végétale bioréacteur microscopie http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_27607 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_3247 http://aims.fao.org/aos/agrovoc/c_3712 http://aims.fao.org/aos/agrovoc/c_33936 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_5954 http://aims.fao.org/aos/agrovoc/c_27469 http://aims.fao.org/aos/agrovoc/c_4810 Nicaragua Costa Rica http://aims.fao.org/aos/agrovoc/c_5171 http://aims.fao.org/aos/agrovoc/c_1920 In Coffea arabica L., the development of direct sowing of somatic embryos (SE) in planting substrate, with subsequent nursery production of plants, has promoted the industrialization of somatic embryogenesis. However, plant conversion rates are still low and require improvements to enhance the cost-effectiveness of commercial micropropagation. With the aim of improving plant regeneration from SE, we studied the morphological and histological criteria and water characteristics during germination and plant conversion of zygotic embryos (ZE) and SE. At the cotyledonary stage, SE produced in a 1 l RITA® temporary immersion bioreactor (area 55.8 cm2) were morphologically similar in size (2-3 mm) but abnormal as compared with mature ZE. Protein and starch reserve levels were extremely low throughout germination and conversion to plantlets, while the water status remained steady [water content (WC) from 76 to 87%, ? from ?0.37 to ?0.47 MPa, pressure potential from 0.69 to 0.24 MPa]. In ZE, spectacular hydration occurred during the first 3 weeks (WC from 37 to 75%; ? from ?6.24 to ?1.0 MPa). Cotyledons remained undifferentiated for 10 weeks after sowing. Conversely, after only 3 weeks under germination conditions in a RITA® bioreactor, spongy and palisade parenchyma and stomata formed in SE cotyledons. The ZE plant conversion was faster than that of SE (14 vs. 22 weeks) and more efficient (rates 96 vs. 55%), with much more substantial hypocotyl and cotyledon development. The use of a new 5 l MATIS® bioreactor (area 355 cm2), designed especially to favor embryo dispersion and light transmittance to SE, markedly improved the embryo-to-plantlet conversion rate (91%). These results highlight the morphological heterogeneity and lack of protein reserves in SE at the beginning of the germination phase and marked differences in water characteristics. However, they also reveal high phenotypic plasticity, leading to a highly efficient plantlet conversion rate due to better embryo dispersion and light transmittance in more horizontal bioreactors. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/569804/1/document_569804.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1093/treephys/tpt034 10.1093/treephys/tpt034 info:eu-repo/semantics/altIdentifier/doi/10.1093/treephys/tpt034 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1093/treephys/tpt034 |