Natural genetic variation of Xanthomonas campestris pv. campestris pathogenicity on Arabidopsis revealed by association and reverse genetics
The pathogenic bacterium Xanthomonas campestris pv. campestris, the causal agent of black rot of Brassicaceae, manipulates the physiology and the innate immunity of its hosts. Association genetic and reverse-genetic analyses of a world panel of 45 X. campestris pv. campestris strains were used to gain understanding of the genetic basis of the bacterium's pathogenicity to Arabidopsis thaliana. We found that the compositions of the minimal predicted type III secretome varied extensively, with 18 to 28 proteins per strain. There were clear differences in aggressiveness of those X. campestris pv. campestris strains on two Arabidopsis natural accessions. We identified 3 effector genes (xopAC, xopJ5, and xopAL2) and 67 amplified fragment length polymorphism (AFLP) markers that were associated with variations in disease symptoms. The nature and distribution of the AFLP markers remain to be determined, but we observed a low linkage disequilibrium level between predicted effectors and other significant markers, suggesting that additional genetic factors make a meaningful contribution to pathogenicity. Mutagenesis of type III effectors in X. campestris pv. campestris confirmed that xopAC functions as both a virulence and an avirulence gene in Arabidopsis and that xopAM functions as a second avirulence gene on plants of the Col-0 ecotype. However, we did not detect the effect of any other effector in the X. campestris pv. campestris 8004 strain, likely due to other genetic background effects. These results highlight the complex genetic basis of pathogenicity at the pathovar level and encourage us to challenge the agronomical relevance of some virulence determinants identified solely in model strains.
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dig-cirad-fr-5693282022-03-30T14:51:39Z http://agritrop.cirad.fr/569328/ http://agritrop.cirad.fr/569328/ Natural genetic variation of Xanthomonas campestris pv. campestris pathogenicity on Arabidopsis revealed by association and reverse genetics. Guy Endrick, Genissel Anne, Hajri Ahmed, Chabannes Matthieu, David Perrine, Carrère Sébastien, Lautier Martine, Roux Brice, Boureau Tristan, Arlat Mathieu, Poussier Stéphane, Noel Laurent D.. 2013. Mbio, 4 (3) : 00538-12.https://doi.org/10.1128/mBio.00538-12 <https://doi.org/10.1128/mBio.00538-12> Researchers Natural genetic variation of Xanthomonas campestris pv. campestris pathogenicity on Arabidopsis revealed by association and reverse genetics Guy, Endrick Genissel, Anne Hajri, Ahmed Chabannes, Matthieu David, Perrine Carrère, Sébastien Lautier, Martine Roux, Brice Boureau, Tristan Arlat, Mathieu Poussier, Stéphane Noel, Laurent D. eng 2013 Mbio H20 - Maladies des plantes The pathogenic bacterium Xanthomonas campestris pv. campestris, the causal agent of black rot of Brassicaceae, manipulates the physiology and the innate immunity of its hosts. Association genetic and reverse-genetic analyses of a world panel of 45 X. campestris pv. campestris strains were used to gain understanding of the genetic basis of the bacterium's pathogenicity to Arabidopsis thaliana. We found that the compositions of the minimal predicted type III secretome varied extensively, with 18 to 28 proteins per strain. There were clear differences in aggressiveness of those X. campestris pv. campestris strains on two Arabidopsis natural accessions. We identified 3 effector genes (xopAC, xopJ5, and xopAL2) and 67 amplified fragment length polymorphism (AFLP) markers that were associated with variations in disease symptoms. The nature and distribution of the AFLP markers remain to be determined, but we observed a low linkage disequilibrium level between predicted effectors and other significant markers, suggesting that additional genetic factors make a meaningful contribution to pathogenicity. Mutagenesis of type III effectors in X. campestris pv. campestris confirmed that xopAC functions as both a virulence and an avirulence gene in Arabidopsis and that xopAM functions as a second avirulence gene on plants of the Col-0 ecotype. However, we did not detect the effect of any other effector in the X. campestris pv. campestris 8004 strain, likely due to other genetic background effects. These results highlight the complex genetic basis of pathogenicity at the pathovar level and encourage us to challenge the agronomical relevance of some virulence determinants identified solely in model strains. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/569328/1/document_569328.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1128/mBio.00538-12 10.1128/mBio.00538-12 info:eu-repo/semantics/altIdentifier/doi/10.1128/mBio.00538-12 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1128/mBio.00538-12 |
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H20 - Maladies des plantes H20 - Maladies des plantes Guy, Endrick Genissel, Anne Hajri, Ahmed Chabannes, Matthieu David, Perrine Carrère, Sébastien Lautier, Martine Roux, Brice Boureau, Tristan Arlat, Mathieu Poussier, Stéphane Noel, Laurent D. Natural genetic variation of Xanthomonas campestris pv. campestris pathogenicity on Arabidopsis revealed by association and reverse genetics |
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The pathogenic bacterium Xanthomonas campestris pv. campestris, the causal agent of black rot of Brassicaceae, manipulates the physiology and the innate immunity of its hosts. Association genetic and reverse-genetic analyses of a world panel of 45 X. campestris pv. campestris strains were used to gain understanding of the genetic basis of the bacterium's pathogenicity to Arabidopsis thaliana. We found that the compositions of the minimal predicted type III secretome varied extensively, with 18 to 28 proteins per strain. There were clear differences in aggressiveness of those X. campestris pv. campestris strains on two Arabidopsis natural accessions. We identified 3 effector genes (xopAC, xopJ5, and xopAL2) and 67 amplified fragment length polymorphism (AFLP) markers that were associated with variations in disease symptoms. The nature and distribution of the AFLP markers remain to be determined, but we observed a low linkage disequilibrium level between predicted effectors and other significant markers, suggesting that additional genetic factors make a meaningful contribution to pathogenicity. Mutagenesis of type III effectors in X. campestris pv. campestris confirmed that xopAC functions as both a virulence and an avirulence gene in Arabidopsis and that xopAM functions as a second avirulence gene on plants of the Col-0 ecotype. However, we did not detect the effect of any other effector in the X. campestris pv. campestris 8004 strain, likely due to other genetic background effects. These results highlight the complex genetic basis of pathogenicity at the pathovar level and encourage us to challenge the agronomical relevance of some virulence determinants identified solely in model strains. |
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article |
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H20 - Maladies des plantes |
author |
Guy, Endrick Genissel, Anne Hajri, Ahmed Chabannes, Matthieu David, Perrine Carrère, Sébastien Lautier, Martine Roux, Brice Boureau, Tristan Arlat, Mathieu Poussier, Stéphane Noel, Laurent D. |
author_facet |
Guy, Endrick Genissel, Anne Hajri, Ahmed Chabannes, Matthieu David, Perrine Carrère, Sébastien Lautier, Martine Roux, Brice Boureau, Tristan Arlat, Mathieu Poussier, Stéphane Noel, Laurent D. |
author_sort |
Guy, Endrick |
title |
Natural genetic variation of Xanthomonas campestris pv. campestris pathogenicity on Arabidopsis revealed by association and reverse genetics |
title_short |
Natural genetic variation of Xanthomonas campestris pv. campestris pathogenicity on Arabidopsis revealed by association and reverse genetics |
title_full |
Natural genetic variation of Xanthomonas campestris pv. campestris pathogenicity on Arabidopsis revealed by association and reverse genetics |
title_fullStr |
Natural genetic variation of Xanthomonas campestris pv. campestris pathogenicity on Arabidopsis revealed by association and reverse genetics |
title_full_unstemmed |
Natural genetic variation of Xanthomonas campestris pv. campestris pathogenicity on Arabidopsis revealed by association and reverse genetics |
title_sort |
natural genetic variation of xanthomonas campestris pv. campestris pathogenicity on arabidopsis revealed by association and reverse genetics |
url |
http://agritrop.cirad.fr/569328/ http://agritrop.cirad.fr/569328/1/document_569328.pdf |
work_keys_str_mv |
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