Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves
A reverse transcriptase (RT)-nested-polymerase chain reaction (PCR) was standardised to detect bovine respiratory syncytial virus (BRSV), using a Brazilian isolate, in three experimentally infected calves. This followed initial tests in infected chicken embryo related (CER) cells. One animal had lesions, characterized by interstitial multifocal pneumonia, severe interstitial and subpleural emphysema, and lung consolidated areas. Lung and tracheal tissues collected 6 days after infection were analysed by RT-nested-PCR. Primers, specific for the BRSV G and F glycoproteins genes, yielded amplification fragments of 371 and 481 bp, respectively, from the RNA of the cell-propagated virus. Using RNA extracted from organs of infected calves, RTnested- PCR amplified the fragment of the G gene in all tracheal samples, but in only two of three lung samples analysed. These results suggest that RT-nested-PCR could be a promising assay for diagnosis and epidemiological analysis of BRSV in Brazil.
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dig-cirad-fr-5687992022-04-21T07:51:22Z http://agritrop.cirad.fr/568799/ http://agritrop.cirad.fr/568799/ Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves. Servan de Almeida Renata, Spilki Fernando Rosado, Roehe Paulo Michel, Arns Clarice Weis. 2005. Veterinary Microbiology, 105 (2) : 131-135.https://doi.org/10.1016/j.vetmic.2004.11.004 <https://doi.org/10.1016/j.vetmic.2004.11.004> Researchers Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves Servan de Almeida, Renata Spilki, Fernando Rosado Roehe, Paulo Michel Arns, Clarice Weis eng 2005 Veterinary Microbiology L73 - Maladies des animaux Brésil http://aims.fao.org/aos/agrovoc/c_1070 A reverse transcriptase (RT)-nested-polymerase chain reaction (PCR) was standardised to detect bovine respiratory syncytial virus (BRSV), using a Brazilian isolate, in three experimentally infected calves. This followed initial tests in infected chicken embryo related (CER) cells. One animal had lesions, characterized by interstitial multifocal pneumonia, severe interstitial and subpleural emphysema, and lung consolidated areas. Lung and tracheal tissues collected 6 days after infection were analysed by RT-nested-PCR. Primers, specific for the BRSV G and F glycoproteins genes, yielded amplification fragments of 371 and 481 bp, respectively, from the RNA of the cell-propagated virus. Using RNA extracted from organs of infected calves, RTnested- PCR amplified the fragment of the G gene in all tracheal samples, but in only two of three lung samples analysed. These results suggest that RT-nested-PCR could be a promising assay for diagnosis and epidemiological analysis of BRSV in Brazil. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/568799/1/document_568799.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.vetmic.2004.11.004 10.1016/j.vetmic.2004.11.004 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2004.11.004 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.vetmic.2004.11.004 |
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L73 - Maladies des animaux http://aims.fao.org/aos/agrovoc/c_1070 L73 - Maladies des animaux http://aims.fao.org/aos/agrovoc/c_1070 Servan de Almeida, Renata Spilki, Fernando Rosado Roehe, Paulo Michel Arns, Clarice Weis Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves |
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A reverse transcriptase (RT)-nested-polymerase chain reaction (PCR) was standardised to detect bovine respiratory syncytial virus (BRSV), using a Brazilian isolate, in three experimentally infected calves. This followed initial tests in infected chicken embryo related (CER) cells. One animal had lesions, characterized by interstitial multifocal pneumonia, severe interstitial and subpleural emphysema, and lung consolidated areas. Lung and tracheal tissues collected 6 days after infection were analysed by RT-nested-PCR. Primers, specific for the BRSV G and F glycoproteins genes, yielded amplification fragments of 371 and 481 bp, respectively, from the RNA of the cell-propagated virus. Using RNA extracted from organs of infected calves, RTnested- PCR amplified the fragment of the G gene in all tracheal samples, but in only two of three lung samples analysed. These results suggest that RT-nested-PCR could be a promising assay for diagnosis and epidemiological analysis of BRSV in Brazil. |
format |
article |
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L73 - Maladies des animaux http://aims.fao.org/aos/agrovoc/c_1070 |
author |
Servan de Almeida, Renata Spilki, Fernando Rosado Roehe, Paulo Michel Arns, Clarice Weis |
author_facet |
Servan de Almeida, Renata Spilki, Fernando Rosado Roehe, Paulo Michel Arns, Clarice Weis |
author_sort |
Servan de Almeida, Renata |
title |
Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves |
title_short |
Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves |
title_full |
Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves |
title_fullStr |
Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves |
title_full_unstemmed |
Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves |
title_sort |
detection of brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves |
url |
http://agritrop.cirad.fr/568799/ http://agritrop.cirad.fr/568799/1/document_568799.pdf |
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