Conservation of coconut (Cocos nucifera L.) germplasm at sub-zero temperature
Protocols are proposed for the low (-20°C) and ultra-low (-80°C) temperature storage of coconut (Cocos nucifera L.) embryos. A tissue dehydration step prior to storage, and a rapid warming step upon recovery optimized the protocol. The thermal properties of water located within the embryos were monitored using differential scanning calorimetry (DSC). In the most efficient version of the protocol, embryos were dehydrated under a sterile air flow in a dehydration solution containing glucose (3.33 M) and glycerol (15%) for16 hours. This protocol decreased the embryo water content from 77 to 29% FW and at the same time reduced the amount of freezable water down to 0.03%. The dehydrated embryos could be stored for up to 3 weeks at -20°C (12 % producing normal plants upon recovery) or 26 weeks at -80°C (28% producing normal plants upon recovery). These results indicate that it is possible to store coconut germplasm on a medium term basis using an ultra-deep freezer unit. However for more efficient, long term storage, cryopreservation remains the preferred option.
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dig-cirad-fr-5682962024-01-28T21:17:21Z http://agritrop.cirad.fr/568296/ http://agritrop.cirad.fr/568296/ Conservation of coconut (Cocos nucifera L.) germplasm at sub-zero temperature. Sisunandar, Sopade Peter A., Samosir Yohannes M.S., Rival Alain, Adkins Steve W.. 2012. CryoLetters, 33 (6) : 465-475. Conservation of coconut (Cocos nucifera L.) germplasm at sub-zero temperature Sisunandar, Sopade, Peter A. Samosir, Yohannes M.S. Rival, Alain Adkins, Steve W. eng 2012 CryoLetters F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F01 - Culture des plantes Cocos nucifera culture d'embryon cryoconservation embryon végétal conservation du matériel génétique germoplasme froid calorimétrie séchage aptitude à la conservation méthodologie http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_24155 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_3249 http://aims.fao.org/aos/agrovoc/c_1743 http://aims.fao.org/aos/agrovoc/c_28276 http://aims.fao.org/aos/agrovoc/c_2402 http://aims.fao.org/aos/agrovoc/c_4082 http://aims.fao.org/aos/agrovoc/c_12522 Philippines Australie http://aims.fao.org/aos/agrovoc/c_5783 http://aims.fao.org/aos/agrovoc/c_714 Protocols are proposed for the low (-20°C) and ultra-low (-80°C) temperature storage of coconut (Cocos nucifera L.) embryos. A tissue dehydration step prior to storage, and a rapid warming step upon recovery optimized the protocol. The thermal properties of water located within the embryos were monitored using differential scanning calorimetry (DSC). In the most efficient version of the protocol, embryos were dehydrated under a sterile air flow in a dehydration solution containing glucose (3.33 M) and glycerol (15%) for16 hours. This protocol decreased the embryo water content from 77 to 29% FW and at the same time reduced the amount of freezable water down to 0.03%. The dehydrated embryos could be stored for up to 3 weeks at -20°C (12 % producing normal plants upon recovery) or 26 weeks at -80°C (28% producing normal plants upon recovery). These results indicate that it is possible to store coconut germplasm on a medium term basis using an ultra-deep freezer unit. However for more efficient, long term storage, cryopreservation remains the preferred option. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/568296/1/document_568296.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F01 - Culture des plantes Cocos nucifera culture d'embryon cryoconservation embryon végétal conservation du matériel génétique germoplasme froid calorimétrie séchage aptitude à la conservation méthodologie http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_24155 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_3249 http://aims.fao.org/aos/agrovoc/c_1743 http://aims.fao.org/aos/agrovoc/c_28276 http://aims.fao.org/aos/agrovoc/c_2402 http://aims.fao.org/aos/agrovoc/c_4082 http://aims.fao.org/aos/agrovoc/c_12522 http://aims.fao.org/aos/agrovoc/c_5783 http://aims.fao.org/aos/agrovoc/c_714 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F01 - Culture des plantes Cocos nucifera culture d'embryon cryoconservation embryon végétal conservation du matériel génétique germoplasme froid calorimétrie séchage aptitude à la conservation méthodologie http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_24155 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_3249 http://aims.fao.org/aos/agrovoc/c_1743 http://aims.fao.org/aos/agrovoc/c_28276 http://aims.fao.org/aos/agrovoc/c_2402 http://aims.fao.org/aos/agrovoc/c_4082 http://aims.fao.org/aos/agrovoc/c_12522 http://aims.fao.org/aos/agrovoc/c_5783 http://aims.fao.org/aos/agrovoc/c_714 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F01 - Culture des plantes Cocos nucifera culture d'embryon cryoconservation embryon végétal conservation du matériel génétique germoplasme froid calorimétrie séchage aptitude à la conservation méthodologie http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_24155 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_3249 http://aims.fao.org/aos/agrovoc/c_1743 http://aims.fao.org/aos/agrovoc/c_28276 http://aims.fao.org/aos/agrovoc/c_2402 http://aims.fao.org/aos/agrovoc/c_4082 http://aims.fao.org/aos/agrovoc/c_12522 http://aims.fao.org/aos/agrovoc/c_5783 http://aims.fao.org/aos/agrovoc/c_714 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F01 - Culture des plantes Cocos nucifera culture d'embryon cryoconservation embryon végétal conservation du matériel génétique germoplasme froid calorimétrie séchage aptitude à la conservation méthodologie http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_24155 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_3249 http://aims.fao.org/aos/agrovoc/c_1743 http://aims.fao.org/aos/agrovoc/c_28276 http://aims.fao.org/aos/agrovoc/c_2402 http://aims.fao.org/aos/agrovoc/c_4082 http://aims.fao.org/aos/agrovoc/c_12522 http://aims.fao.org/aos/agrovoc/c_5783 http://aims.fao.org/aos/agrovoc/c_714 Sisunandar, Sopade, Peter A. Samosir, Yohannes M.S. Rival, Alain Adkins, Steve W. Conservation of coconut (Cocos nucifera L.) germplasm at sub-zero temperature |
description |
Protocols are proposed for the low (-20°C) and ultra-low (-80°C) temperature storage of coconut (Cocos nucifera L.) embryos. A tissue dehydration step prior to storage, and a rapid warming step upon recovery optimized the protocol. The thermal properties of water located within the embryos were monitored using differential scanning calorimetry (DSC). In the most efficient version of the protocol, embryos were dehydrated under a sterile air flow in a dehydration solution containing glucose (3.33 M) and glycerol (15%) for16 hours. This protocol decreased the embryo water content from 77 to 29% FW and at the same time reduced the amount of freezable water down to 0.03%. The dehydrated embryos could be stored for up to 3 weeks at -20°C (12 % producing normal plants upon recovery) or 26 weeks at -80°C (28% producing normal plants upon recovery). These results indicate that it is possible to store coconut germplasm on a medium term basis using an ultra-deep freezer unit. However for more efficient, long term storage, cryopreservation remains the preferred option. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale F01 - Culture des plantes Cocos nucifera culture d'embryon cryoconservation embryon végétal conservation du matériel génétique germoplasme froid calorimétrie séchage aptitude à la conservation méthodologie http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_24155 http://aims.fao.org/aos/agrovoc/c_2543 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_3249 http://aims.fao.org/aos/agrovoc/c_1743 http://aims.fao.org/aos/agrovoc/c_28276 http://aims.fao.org/aos/agrovoc/c_2402 http://aims.fao.org/aos/agrovoc/c_4082 http://aims.fao.org/aos/agrovoc/c_12522 http://aims.fao.org/aos/agrovoc/c_5783 http://aims.fao.org/aos/agrovoc/c_714 |
author |
Sisunandar, Sopade, Peter A. Samosir, Yohannes M.S. Rival, Alain Adkins, Steve W. |
author_facet |
Sisunandar, Sopade, Peter A. Samosir, Yohannes M.S. Rival, Alain Adkins, Steve W. |
author_sort |
Sisunandar, |
title |
Conservation of coconut (Cocos nucifera L.) germplasm at sub-zero temperature |
title_short |
Conservation of coconut (Cocos nucifera L.) germplasm at sub-zero temperature |
title_full |
Conservation of coconut (Cocos nucifera L.) germplasm at sub-zero temperature |
title_fullStr |
Conservation of coconut (Cocos nucifera L.) germplasm at sub-zero temperature |
title_full_unstemmed |
Conservation of coconut (Cocos nucifera L.) germplasm at sub-zero temperature |
title_sort |
conservation of coconut (cocos nucifera l.) germplasm at sub-zero temperature |
url |
http://agritrop.cirad.fr/568296/ http://agritrop.cirad.fr/568296/1/document_568296.pdf |
work_keys_str_mv |
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