Analysis of the expression of the AGL17-like clade of MADS-box transcription factors in rice
In plants, MADS-box transcription factors are key regulators of floral and fruit development, organ dehiscence and stress responses. Nevertheless, the functions of most of them are still unknown. In Arabidopsis thaliana, the AGL17-like clade of MADS-box transcription factors comprises four members. AGL17 is involved in floral induction, whereas AGL44/ANR1 is involved in the adaptive development of roots in response to nitrate. AGL21 is primarily expressed in the roots and AGL16 in the leaves, suggesting that these transcription factors may be involved in the control of vegetative development. In Oryza sativa, the AGL17-like clade comprises five members, OsMADS23, OsMADS25, OsMADS27, OsMADS57 and OsMADS61. In a first attempt to characterize their functions, we used promoter::Gus reporter gene fusions and RT-qPCR to study the expression patterns of these genes and their regulation by different external stimuli. The OsMADS23, OsMADS25, OsMADS27 and OsMADS57 promoters were active in the root's central cylinder. In addition, the OsMADS57 promoter was active in leaves, whereas the OsMADS61 promoter was only active in the leaf tips and the stem base. OsMADS25 and OsMADS27 transcripts accumulated in response to osmotic stress, whereas the expression levels of OsMADS25, OsMADS27 and OsMADS57 were slightly induced by nitrate. Each of these five genes was responsive to various hormonal treatments. These distinct expression patterns indicate that these five genes have specific and non-redundant functions that likely differs from those of their A. thaliana homologs.
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Subjects: | F30 - Génétique et amélioration des plantes, F60 - Physiologie et biochimie végétale, Oryza sativa, Arabidopsis thaliana, expression des gènes, séquence nucléotidique, transcription, azote, stress osmotique, hormone, génie génétique, plante transgénique, amélioration des plantes, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_33292, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_35126, http://aims.fao.org/aos/agrovoc/c_5192, http://aims.fao.org/aos/agrovoc/c_35750, http://aims.fao.org/aos/agrovoc/c_3663, http://aims.fao.org/aos/agrovoc/c_15974, http://aims.fao.org/aos/agrovoc/c_27619, http://aims.fao.org/aos/agrovoc/c_5956, |
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dig-cirad-fr-5682942024-01-28T21:17:19Z http://agritrop.cirad.fr/568294/ http://agritrop.cirad.fr/568294/ Analysis of the expression of the AGL17-like clade of MADS-box transcription factors in rice. Puig Jérôme, Meynard Donaldo, Khong Ngan Giang, Pauluzzi Germain, Guiderdoni Emmanuel, Gantet Pascal. 2013. Gene Expression Patterns, 13 (5-6) : 160-170.https://doi.org/10.1016/j.gep.2013.02.004 <https://doi.org/10.1016/j.gep.2013.02.004> Analysis of the expression of the AGL17-like clade of MADS-box transcription factors in rice Puig, Jérôme Meynard, Donaldo Khong, Ngan Giang Pauluzzi, Germain Guiderdoni, Emmanuel Gantet, Pascal eng 2013 Gene Expression Patterns F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Oryza sativa Arabidopsis thaliana expression des gènes séquence nucléotidique transcription azote stress osmotique hormone génie génétique plante transgénique amélioration des plantes http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_5192 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_3663 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5956 In plants, MADS-box transcription factors are key regulators of floral and fruit development, organ dehiscence and stress responses. Nevertheless, the functions of most of them are still unknown. In Arabidopsis thaliana, the AGL17-like clade of MADS-box transcription factors comprises four members. AGL17 is involved in floral induction, whereas AGL44/ANR1 is involved in the adaptive development of roots in response to nitrate. AGL21 is primarily expressed in the roots and AGL16 in the leaves, suggesting that these transcription factors may be involved in the control of vegetative development. In Oryza sativa, the AGL17-like clade comprises five members, OsMADS23, OsMADS25, OsMADS27, OsMADS57 and OsMADS61. In a first attempt to characterize their functions, we used promoter::Gus reporter gene fusions and RT-qPCR to study the expression patterns of these genes and their regulation by different external stimuli. The OsMADS23, OsMADS25, OsMADS27 and OsMADS57 promoters were active in the root's central cylinder. In addition, the OsMADS57 promoter was active in leaves, whereas the OsMADS61 promoter was only active in the leaf tips and the stem base. OsMADS25 and OsMADS27 transcripts accumulated in response to osmotic stress, whereas the expression levels of OsMADS25, OsMADS27 and OsMADS57 were slightly induced by nitrate. Each of these five genes was responsive to various hormonal treatments. These distinct expression patterns indicate that these five genes have specific and non-redundant functions that likely differs from those of their A. thaliana homologs. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/568294/1/document_568294.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.gep.2013.02.004 10.1016/j.gep.2013.02.004 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gep.2013.02.004 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.gep.2013.02.004 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Oryza sativa Arabidopsis thaliana expression des gènes séquence nucléotidique transcription azote stress osmotique hormone génie génétique plante transgénique amélioration des plantes http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_5192 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_3663 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5956 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Oryza sativa Arabidopsis thaliana expression des gènes séquence nucléotidique transcription azote stress osmotique hormone génie génétique plante transgénique amélioration des plantes http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_5192 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_3663 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5956 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Oryza sativa Arabidopsis thaliana expression des gènes séquence nucléotidique transcription azote stress osmotique hormone génie génétique plante transgénique amélioration des plantes http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_5192 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_3663 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5956 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Oryza sativa Arabidopsis thaliana expression des gènes séquence nucléotidique transcription azote stress osmotique hormone génie génétique plante transgénique amélioration des plantes http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_5192 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_3663 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5956 Puig, Jérôme Meynard, Donaldo Khong, Ngan Giang Pauluzzi, Germain Guiderdoni, Emmanuel Gantet, Pascal Analysis of the expression of the AGL17-like clade of MADS-box transcription factors in rice |
description |
In plants, MADS-box transcription factors are key regulators of floral and fruit development, organ dehiscence and stress responses. Nevertheless, the functions of most of them are still unknown. In Arabidopsis thaliana, the AGL17-like clade of MADS-box transcription factors comprises four members. AGL17 is involved in floral induction, whereas AGL44/ANR1 is involved in the adaptive development of roots in response to nitrate. AGL21 is primarily expressed in the roots and AGL16 in the leaves, suggesting that these transcription factors may be involved in the control of vegetative development. In Oryza sativa, the AGL17-like clade comprises five members, OsMADS23, OsMADS25, OsMADS27, OsMADS57 and OsMADS61. In a first attempt to characterize their functions, we used promoter::Gus reporter gene fusions and RT-qPCR to study the expression patterns of these genes and their regulation by different external stimuli. The OsMADS23, OsMADS25, OsMADS27 and OsMADS57 promoters were active in the root's central cylinder. In addition, the OsMADS57 promoter was active in leaves, whereas the OsMADS61 promoter was only active in the leaf tips and the stem base. OsMADS25 and OsMADS27 transcripts accumulated in response to osmotic stress, whereas the expression levels of OsMADS25, OsMADS27 and OsMADS57 were slightly induced by nitrate. Each of these five genes was responsive to various hormonal treatments. These distinct expression patterns indicate that these five genes have specific and non-redundant functions that likely differs from those of their A. thaliana homologs. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Oryza sativa Arabidopsis thaliana expression des gènes séquence nucléotidique transcription azote stress osmotique hormone génie génétique plante transgénique amélioration des plantes http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_5192 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_3663 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5956 |
author |
Puig, Jérôme Meynard, Donaldo Khong, Ngan Giang Pauluzzi, Germain Guiderdoni, Emmanuel Gantet, Pascal |
author_facet |
Puig, Jérôme Meynard, Donaldo Khong, Ngan Giang Pauluzzi, Germain Guiderdoni, Emmanuel Gantet, Pascal |
author_sort |
Puig, Jérôme |
title |
Analysis of the expression of the AGL17-like clade of MADS-box transcription factors in rice |
title_short |
Analysis of the expression of the AGL17-like clade of MADS-box transcription factors in rice |
title_full |
Analysis of the expression of the AGL17-like clade of MADS-box transcription factors in rice |
title_fullStr |
Analysis of the expression of the AGL17-like clade of MADS-box transcription factors in rice |
title_full_unstemmed |
Analysis of the expression of the AGL17-like clade of MADS-box transcription factors in rice |
title_sort |
analysis of the expression of the agl17-like clade of mads-box transcription factors in rice |
url |
http://agritrop.cirad.fr/568294/ http://agritrop.cirad.fr/568294/1/document_568294.pdf |
work_keys_str_mv |
AT puigjerome analysisoftheexpressionoftheagl17likecladeofmadsboxtranscriptionfactorsinrice AT meynarddonaldo analysisoftheexpressionoftheagl17likecladeofmadsboxtranscriptionfactorsinrice AT khongngangiang analysisoftheexpressionoftheagl17likecladeofmadsboxtranscriptionfactorsinrice AT pauluzzigermain analysisoftheexpressionoftheagl17likecladeofmadsboxtranscriptionfactorsinrice AT guiderdoniemmanuel analysisoftheexpressionoftheagl17likecladeofmadsboxtranscriptionfactorsinrice AT gantetpascal analysisoftheexpressionoftheagl17likecladeofmadsboxtranscriptionfactorsinrice |
_version_ |
1792498426235060224 |