Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus
Background: Single-stranded (ss) DNA viruses in the family Geminiviridae are proving to be very useful in real-time evolution studies. The high mutation rate of geminiviruses and other ssDNA viruses is somewhat mysterious in that their DNA genomes are replicated in host nuclei by high fidelity host polymerases. Although strand specific mutation biases observed in virus species from the geminivirus genus Mastrevirus indicate that the high mutation rates in viruses in this genus may be due to mutational processes that operate specifically on ssDNA, it is currently unknown whether viruses from other genera display similar strand specific mutation biases. Also, geminivirus genomes frequently recombine with one another and an alternative cause of their high mutation rates could be that the recombination process is either directly mutagenic or produces a selective environment in which the survival of mutants is favoured. To investigate whether there is an association between recombination and increased basal mutation rates or increased degrees of selection favoring the survival of mutations, we compared the mutation dynamics of the MSV-MatA and MSV-VW field isolates of Maize streak virus (MSV; Mastrevirus), with both a laboratory constructed MSV recombinant, and MSV recombinants closely resembling MSV-MatA. To determine whether strand specific mutation biases are a general characteristic of geminivirus evolution we compared mutation spectra arising during these MSV experiments with those arising during similar experiments involving the geminivirus Tomato yellow leaf curl virus (Begomovirus genus). Results: Although both the genomic distribution of mutations and the occurrence of various convergent mutations at specific genomic sites indicated that either mutation hotspots or selection for adaptive mutations might elevate observed mutation rates in MSV, we found no association between recombination and mutation rates. Importantly, when comparing the mutation spectra of MSV and TYLCV we observed similar strand specific mutation biases arising predominantly from imbalances in the complementary mutations G?T: C?A. Conclusions: While our results suggest that recombination does not strongly influence mutation rates in MSV, they indicate that high geminivirus mutation rates are at least partially attributable to increased susceptibility of all geminivirus genomes to oxidative damage while in a single stranded state.
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Subjects: | H20 - Maladies des plantes, virus striure du maïs, géminivirus enroulement jaune tomat, recombinaison, mutation, adaptation, évolution, http://aims.fao.org/aos/agrovoc/c_33375, http://aims.fao.org/aos/agrovoc/c_32611, http://aims.fao.org/aos/agrovoc/c_6475, http://aims.fao.org/aos/agrovoc/c_5014, http://aims.fao.org/aos/agrovoc/c_117, http://aims.fao.org/aos/agrovoc/c_2745, http://aims.fao.org/aos/agrovoc/c_7252, |
Online Access: | http://agritrop.cirad.fr/567852/ http://agritrop.cirad.fr/567852/1/document_567852.pdf |
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H20 - Maladies des plantes virus striure du maïs géminivirus enroulement jaune tomat recombinaison mutation adaptation évolution http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_32611 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_7252 H20 - Maladies des plantes virus striure du maïs géminivirus enroulement jaune tomat recombinaison mutation adaptation évolution http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_32611 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_7252 |
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H20 - Maladies des plantes virus striure du maïs géminivirus enroulement jaune tomat recombinaison mutation adaptation évolution http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_32611 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_7252 H20 - Maladies des plantes virus striure du maïs géminivirus enroulement jaune tomat recombinaison mutation adaptation évolution http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_32611 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_7252 Monjane, Adérito Luis Pande, Daniel Lakay, Francisco Shepherd, Dionne Natalie Van der Walt, Eric Lefeuvre, Pierre Lett, Jean-Michel Varsani, Arvind Rybicki, Edward P. Martin, Darren Patrick Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus |
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Background: Single-stranded (ss) DNA viruses in the family Geminiviridae are proving to be very useful in real-time evolution studies. The high mutation rate of geminiviruses and other ssDNA viruses is somewhat mysterious in that their DNA genomes are replicated in host nuclei by high fidelity host polymerases. Although strand specific mutation biases observed in virus species from the geminivirus genus Mastrevirus indicate that the high mutation rates in viruses in this genus may be due to mutational processes that operate specifically on ssDNA, it is currently unknown whether viruses from other genera display similar strand specific mutation biases. Also, geminivirus genomes frequently recombine with one another and an alternative cause of their high mutation rates could be that the recombination process is either directly mutagenic or produces a selective environment in which the survival of mutants is favoured. To investigate whether there is an association between recombination and increased basal mutation rates or increased degrees of selection favoring the survival of mutations, we compared the mutation dynamics of the MSV-MatA and MSV-VW field isolates of Maize streak virus (MSV; Mastrevirus), with both a laboratory constructed MSV recombinant, and MSV recombinants closely resembling MSV-MatA. To determine whether strand specific mutation biases are a general characteristic of geminivirus evolution we compared mutation spectra arising during these MSV experiments with those arising during similar experiments involving the geminivirus Tomato yellow leaf curl virus (Begomovirus genus). Results: Although both the genomic distribution of mutations and the occurrence of various convergent mutations at specific genomic sites indicated that either mutation hotspots or selection for adaptive mutations might elevate observed mutation rates in MSV, we found no association between recombination and mutation rates. Importantly, when comparing the mutation spectra of MSV and TYLCV we observed similar strand specific mutation biases arising predominantly from imbalances in the complementary mutations G?T: C?A. Conclusions: While our results suggest that recombination does not strongly influence mutation rates in MSV, they indicate that high geminivirus mutation rates are at least partially attributable to increased susceptibility of all geminivirus genomes to oxidative damage while in a single stranded state. |
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H20 - Maladies des plantes virus striure du maïs géminivirus enroulement jaune tomat recombinaison mutation adaptation évolution http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_32611 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_7252 |
author |
Monjane, Adérito Luis Pande, Daniel Lakay, Francisco Shepherd, Dionne Natalie Van der Walt, Eric Lefeuvre, Pierre Lett, Jean-Michel Varsani, Arvind Rybicki, Edward P. Martin, Darren Patrick |
author_facet |
Monjane, Adérito Luis Pande, Daniel Lakay, Francisco Shepherd, Dionne Natalie Van der Walt, Eric Lefeuvre, Pierre Lett, Jean-Michel Varsani, Arvind Rybicki, Edward P. Martin, Darren Patrick |
author_sort |
Monjane, Adérito Luis |
title |
Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus |
title_short |
Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus |
title_full |
Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus |
title_fullStr |
Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus |
title_full_unstemmed |
Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus |
title_sort |
adaptive evolution by recombination is not associated with increased mutation rates in maize streak virus |
url |
http://agritrop.cirad.fr/567852/ http://agritrop.cirad.fr/567852/1/document_567852.pdf |
work_keys_str_mv |
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_version_ |
1792498413201260544 |
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dig-cirad-fr-5678522024-01-28T21:14:38Z http://agritrop.cirad.fr/567852/ http://agritrop.cirad.fr/567852/ Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus. Monjane Adérito Luis, Pande Daniel, Lakay Francisco, Shepherd Dionne Natalie, Van der Walt Eric, Lefeuvre Pierre, Lett Jean-Michel, Varsani Arvind, Rybicki Edward P., Martin Darren Patrick. 2012. BMC Evolutionary Biology, 12 (252), 13 p.https://doi.org/10.1186/1471-2148-12-252 <https://doi.org/10.1186/1471-2148-12-252> Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus Monjane, Adérito Luis Pande, Daniel Lakay, Francisco Shepherd, Dionne Natalie Van der Walt, Eric Lefeuvre, Pierre Lett, Jean-Michel Varsani, Arvind Rybicki, Edward P. Martin, Darren Patrick eng 2012 BMC Evolutionary Biology H20 - Maladies des plantes virus striure du maïs géminivirus enroulement jaune tomat recombinaison mutation adaptation évolution http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_32611 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_2745 Afrique du Sud http://aims.fao.org/aos/agrovoc/c_7252 Background: Single-stranded (ss) DNA viruses in the family Geminiviridae are proving to be very useful in real-time evolution studies. The high mutation rate of geminiviruses and other ssDNA viruses is somewhat mysterious in that their DNA genomes are replicated in host nuclei by high fidelity host polymerases. Although strand specific mutation biases observed in virus species from the geminivirus genus Mastrevirus indicate that the high mutation rates in viruses in this genus may be due to mutational processes that operate specifically on ssDNA, it is currently unknown whether viruses from other genera display similar strand specific mutation biases. Also, geminivirus genomes frequently recombine with one another and an alternative cause of their high mutation rates could be that the recombination process is either directly mutagenic or produces a selective environment in which the survival of mutants is favoured. To investigate whether there is an association between recombination and increased basal mutation rates or increased degrees of selection favoring the survival of mutations, we compared the mutation dynamics of the MSV-MatA and MSV-VW field isolates of Maize streak virus (MSV; Mastrevirus), with both a laboratory constructed MSV recombinant, and MSV recombinants closely resembling MSV-MatA. To determine whether strand specific mutation biases are a general characteristic of geminivirus evolution we compared mutation spectra arising during these MSV experiments with those arising during similar experiments involving the geminivirus Tomato yellow leaf curl virus (Begomovirus genus). Results: Although both the genomic distribution of mutations and the occurrence of various convergent mutations at specific genomic sites indicated that either mutation hotspots or selection for adaptive mutations might elevate observed mutation rates in MSV, we found no association between recombination and mutation rates. Importantly, when comparing the mutation spectra of MSV and TYLCV we observed similar strand specific mutation biases arising predominantly from imbalances in the complementary mutations G?T: C?A. Conclusions: While our results suggest that recombination does not strongly influence mutation rates in MSV, they indicate that high geminivirus mutation rates are at least partially attributable to increased susceptibility of all geminivirus genomes to oxidative damage while in a single stranded state. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/567852/1/document_567852.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1186/1471-2148-12-252 10.1186/1471-2148-12-252 info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2148-12-252 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/1471-2148-12-252 |