New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars
Cybridization should be a way to induce phenotypical variations in highly heterozygous secondary citrus species or genotypes, without breaking their very complex multilocus structure by sexual recombination. It should have particular interest to manipulate the nucleo-cytoplasmic male sterility, to transfer tolerance traits with cytoplasmic determinisms and eventually to improve tolerance to oxidative stress. The present work is focused on the creation of new cybrids through three different approaches of protoplast fusion: (i) symmetric hybridization, (ii) donor-recipient protoplast fusion by UV irradiation of donor protoplasts, and (iii) cytoplasts + protoplast fusion. Sixteen combinations of symmetric protoplast fusion have produced cybrids. No cybrid was obtained after UV irradiation of donor protoplasts while three combinations of cytoplasts + protoplast fusion produced cybrids. Therefore, cytoplast fusion appears much more promising than the UV approach for targeted cybridization. The innovative plant material is currently propagated for further cultivar and rootstock selection and for basic researches on the impact of nucleo-cytoplasmic interaction on desirable traits.
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dig-cirad-fr-5672912022-08-03T16:02:54Z http://agritrop.cirad.fr/567291/ http://agritrop.cirad.fr/567291/ New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars. Dambier Dominique, Petit Fanny, Barantin Pascal, Ollitrault Patrick. 2015. In : Proceedings of the XIIth International Citrus Congress: International Society of Citriculture. Navarro Luis (ed.), Sabater-Muñoz Beatriz (ed.), Moreno Pedro (ed.), Peña Leandro (ed.). ISHS. Leuven : ISHS, 501-510. (Acta Horticulturae, 1065) ISBN 978-94-62610-53-8 International Citrus Congress: Citrus and Health. 12, Valence, Espagne, 18 Novembre 2012/23 Novembre 2012.http://www.actahort.org/books/1065/1065_61.htm <http://www.actahort.org/books/1065/1065_61.htm> Researchers New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars Dambier, Dominique Petit, Fanny Barantin, Pascal Ollitrault, Patrick eng 2015 ISHS Proceedings of the XIIth International Citrus Congress: International Society of Citriculture F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes Cybridization should be a way to induce phenotypical variations in highly heterozygous secondary citrus species or genotypes, without breaking their very complex multilocus structure by sexual recombination. It should have particular interest to manipulate the nucleo-cytoplasmic male sterility, to transfer tolerance traits with cytoplasmic determinisms and eventually to improve tolerance to oxidative stress. The present work is focused on the creation of new cybrids through three different approaches of protoplast fusion: (i) symmetric hybridization, (ii) donor-recipient protoplast fusion by UV irradiation of donor protoplasts, and (iii) cytoplasts + protoplast fusion. Sixteen combinations of symmetric protoplast fusion have produced cybrids. No cybrid was obtained after UV irradiation of donor protoplasts while three combinations of cytoplasts + protoplast fusion produced cybrids. Therefore, cytoplast fusion appears much more promising than the UV approach for targeted cybridization. The innovative plant material is currently propagated for further cultivar and rootstock selection and for basic researches on the impact of nucleo-cytoplasmic interaction on desirable traits. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/567291/1/document_567291.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://www.actahort.org/books/1065/1065_61.htm http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=215463 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=217857 info:eu-repo/semantics/altIdentifier/purl/http://www.actahort.org/books/1065/1065_61.htm |
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F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes Dambier, Dominique Petit, Fanny Barantin, Pascal Ollitrault, Patrick New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars |
description |
Cybridization should be a way to induce phenotypical variations in highly heterozygous secondary citrus species or genotypes, without breaking their very complex multilocus structure by sexual recombination. It should have particular interest to manipulate the nucleo-cytoplasmic male sterility, to transfer tolerance traits with cytoplasmic determinisms and eventually to improve tolerance to oxidative stress. The present work is focused on the creation of new cybrids through three different approaches of protoplast fusion: (i) symmetric hybridization, (ii) donor-recipient protoplast fusion by UV irradiation of donor protoplasts, and (iii) cytoplasts + protoplast fusion. Sixteen combinations of symmetric protoplast fusion have produced cybrids. No cybrid was obtained after UV irradiation of donor protoplasts while three combinations of cytoplasts + protoplast fusion produced cybrids. Therefore, cytoplast fusion appears much more promising than the UV approach for targeted cybridization. The innovative plant material is currently propagated for further cultivar and rootstock selection and for basic researches on the impact of nucleo-cytoplasmic interaction on desirable traits. |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes |
author |
Dambier, Dominique Petit, Fanny Barantin, Pascal Ollitrault, Patrick |
author_facet |
Dambier, Dominique Petit, Fanny Barantin, Pascal Ollitrault, Patrick |
author_sort |
Dambier, Dominique |
title |
New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars |
title_short |
New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars |
title_full |
New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars |
title_fullStr |
New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars |
title_full_unstemmed |
New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars |
title_sort |
new cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars |
publisher |
ISHS |
url |
http://agritrop.cirad.fr/567291/ http://agritrop.cirad.fr/567291/1/document_567291.pdf |
work_keys_str_mv |
AT dambierdominique newcybridsresultingfromasexualpathwayapromiseofthecybridizationforcreatingnewcitrusrootstocksandcultivars AT petitfanny newcybridsresultingfromasexualpathwayapromiseofthecybridizationforcreatingnewcitrusrootstocksandcultivars AT barantinpascal newcybridsresultingfromasexualpathwayapromiseofthecybridizationforcreatingnewcitrusrootstocksandcultivars AT ollitraultpatrick newcybridsresultingfromasexualpathwayapromiseofthecybridizationforcreatingnewcitrusrootstocksandcultivars |
_version_ |
1758023566087946240 |