Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence
In Arabidopsis, gene expression studies and analysis of knock-out (KO) mutants have been instrumental in building an integrated view of disease resistance pathways. Such an integrated view is missing in rice where shared tools, including genes and mutants, must be assembled. This work provides a tool kit consisting of informative genes for the molecular characterization of the interaction of rice with the major fungal pathogen Magnaporthe oryzae. It also provides for a set of eight KO mutants, all in the same genotypic background, in genes involved in key steps of the rice disease resistance pathway. This study demonstrates the involvement of three genes, OsWRKY28, rTGA2.1 and NH1, in the establishment of full basal resistance to rice blast. The transcription factor OsWRKY28 acts as a negative regulator of basal resistance, like the orthologous barley gene. Finally, the up-regulation of the negative regulator OsWRKY28 and the down-regulation of PR gene expression early during M. oryzae infection suggest that the fungus possesses infection mechanisms that enable it to block host defences.
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Subjects: | F30 - Génétique et amélioration des plantes, H20 - Maladies des plantes, Oryza sativa, Magnaporthe, mutation, expression des gènes, résistance génétique, génie génétique, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_31827, http://aims.fao.org/aos/agrovoc/c_5014, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_35130, http://aims.fao.org/aos/agrovoc/c_15974, |
Online Access: | http://agritrop.cirad.fr/566813/ http://agritrop.cirad.fr/566813/1/document_566813.pdf |
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dig-cirad-fr-5668132024-06-13T18:00:04Z http://agritrop.cirad.fr/566813/ http://agritrop.cirad.fr/566813/ Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence. Delteil Amandine, Blein Mélisande, Faivre-Rampant Odile, Guellim Amira, Estevan Joan, Hirsch Judith, Bevitori Rosangela, Michel Corinne, Morel Jean-Benoit. 2012. Molecular Plant Pathology, 13 (1) : 72-82.https://doi.org/10.1111/j.1364-3703.2011.00731.x <https://doi.org/10.1111/j.1364-3703.2011.00731.x> Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence Delteil, Amandine Blein, Mélisande Faivre-Rampant, Odile Guellim, Amira Estevan, Joan Hirsch, Judith Bevitori, Rosangela Michel, Corinne Morel, Jean-Benoit eng 2012 Molecular Plant Pathology F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa Magnaporthe mutation expression des gènes résistance génétique génie génétique http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15974 In Arabidopsis, gene expression studies and analysis of knock-out (KO) mutants have been instrumental in building an integrated view of disease resistance pathways. Such an integrated view is missing in rice where shared tools, including genes and mutants, must be assembled. This work provides a tool kit consisting of informative genes for the molecular characterization of the interaction of rice with the major fungal pathogen Magnaporthe oryzae. It also provides for a set of eight KO mutants, all in the same genotypic background, in genes involved in key steps of the rice disease resistance pathway. This study demonstrates the involvement of three genes, OsWRKY28, rTGA2.1 and NH1, in the establishment of full basal resistance to rice blast. The transcription factor OsWRKY28 acts as a negative regulator of basal resistance, like the orthologous barley gene. Finally, the up-regulation of the negative regulator OsWRKY28 and the down-regulation of PR gene expression early during M. oryzae infection suggest that the fungus possesses infection mechanisms that enable it to block host defences. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/566813/1/document_566813.pdf application/pdf cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1111/j.1364-3703.2011.00731.x 10.1111/j.1364-3703.2011.00731.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1364-3703.2011.00731.x info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/j.1364-3703.2011.00731.x |
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F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa Magnaporthe mutation expression des gènes résistance génétique génie génétique http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15974 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa Magnaporthe mutation expression des gènes résistance génétique génie génétique http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15974 |
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F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa Magnaporthe mutation expression des gènes résistance génétique génie génétique http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15974 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa Magnaporthe mutation expression des gènes résistance génétique génie génétique http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15974 Delteil, Amandine Blein, Mélisande Faivre-Rampant, Odile Guellim, Amira Estevan, Joan Hirsch, Judith Bevitori, Rosangela Michel, Corinne Morel, Jean-Benoit Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence |
description |
In Arabidopsis, gene expression studies and analysis of knock-out (KO) mutants have been instrumental in building an integrated view of disease resistance pathways. Such an integrated view is missing in rice where shared tools, including genes and mutants, must be assembled. This work provides a tool kit consisting of informative genes for the molecular characterization of the interaction of rice with the major fungal pathogen Magnaporthe oryzae. It also provides for a set of eight KO mutants, all in the same genotypic background, in genes involved in key steps of the rice disease resistance pathway. This study demonstrates the involvement of three genes, OsWRKY28, rTGA2.1 and NH1, in the establishment of full basal resistance to rice blast. The transcription factor OsWRKY28 acts as a negative regulator of basal resistance, like the orthologous barley gene. Finally, the up-regulation of the negative regulator OsWRKY28 and the down-regulation of PR gene expression early during M. oryzae infection suggest that the fungus possesses infection mechanisms that enable it to block host defences. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa Magnaporthe mutation expression des gènes résistance génétique génie génétique http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15974 |
author |
Delteil, Amandine Blein, Mélisande Faivre-Rampant, Odile Guellim, Amira Estevan, Joan Hirsch, Judith Bevitori, Rosangela Michel, Corinne Morel, Jean-Benoit |
author_facet |
Delteil, Amandine Blein, Mélisande Faivre-Rampant, Odile Guellim, Amira Estevan, Joan Hirsch, Judith Bevitori, Rosangela Michel, Corinne Morel, Jean-Benoit |
author_sort |
Delteil, Amandine |
title |
Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence |
title_short |
Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence |
title_full |
Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence |
title_fullStr |
Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence |
title_full_unstemmed |
Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence |
title_sort |
building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence |
url |
http://agritrop.cirad.fr/566813/ http://agritrop.cirad.fr/566813/1/document_566813.pdf |
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