High genetic diversity in field isolates of Trypanosoma theileri assessed by analysis of cathepsin L-like sequences disclosed multiple and new genotypes infecting cattle in Thailand
In this study, we describe the first survey in Thailand of Trypanosoma theileri, a widespread and prevalent parasite of cattle that is transmitted by tabanid flies. Investigation of 210 bovine blood samples of Thai cattle from six farms by hematocrit centrifuge technique (HCT) revealed 14 samples with trypanosomes morphologically compatible to T. theileri. Additional animals were positive for T. theileri by PCR based on the Cathepsin L-like sequence (TthCATL-PCR) despite negative by HCT, indicating cryptic infections. Results revealed a prevalence of 26 ± 15% (95% CI) of T. theileri infection. Additionally, 12 samples positive for T. theileri were detected in cattle from other 11 farms. From a total of 30 blood samples positive by HCT and/or PCR from 17 farms, seven were characterized to evaluate the genetic polymorphism of T. theileri through sequence analysis of PCR-amplified CATL DNA sequences. All CATL sequences of T. theileri from Thai cattle clustered with sequences of the previously described phylogenetic lineages TthI and TthII, supporting only two major lineages of T. theileri in cattle around the world. However, 11 of the 29 CATL sequences analyzed showed to be different, disclosing an unexpectedly large polymorphic genetic repertoire, with multiple genotypes of T. theileri not previously described in other countries circulating in Thai cattle.
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Subjects: | U30 - Méthodes de recherche, L73 - Maladies des animaux, Trypanosoma theileri, variation génétique, trypanosomose, diagnostic, peptidase, gène, PCR, bovin, http://aims.fao.org/aos/agrovoc/c_27407, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_7988, http://aims.fao.org/aos/agrovoc/c_2238, http://aims.fao.org/aos/agrovoc/c_5690, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_1391, http://aims.fao.org/aos/agrovoc/c_7701, |
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dig-cirad-fr-5660372024-01-28T20:50:26Z http://agritrop.cirad.fr/566037/ http://agritrop.cirad.fr/566037/ High genetic diversity in field isolates of Trypanosoma theileri assessed by analysis of cathepsin L-like sequences disclosed multiple and new genotypes infecting cattle in Thailand. Garcia Herakles A., Kamyingkird Ketsarin, Rodrigues Adriana C., Jittapalapong Sathaporn, Teixeira Marta M.G., Desquesnes Marc. 2011. Veterinary Parasitology, 180 (3-4) : 363-367.https://doi.org/10.1016/j.vetpar.2011.03.017 <https://doi.org/10.1016/j.vetpar.2011.03.017> High genetic diversity in field isolates of Trypanosoma theileri assessed by analysis of cathepsin L-like sequences disclosed multiple and new genotypes infecting cattle in Thailand Garcia, Herakles A. Kamyingkird, Ketsarin Rodrigues, Adriana C. Jittapalapong, Sathaporn Teixeira, Marta M.G. Desquesnes, Marc eng 2011 Veterinary Parasitology U30 - Méthodes de recherche L73 - Maladies des animaux Trypanosoma theileri variation génétique trypanosomose diagnostic peptidase gène PCR bovin http://aims.fao.org/aos/agrovoc/c_27407 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_5690 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1391 Thaïlande http://aims.fao.org/aos/agrovoc/c_7701 In this study, we describe the first survey in Thailand of Trypanosoma theileri, a widespread and prevalent parasite of cattle that is transmitted by tabanid flies. Investigation of 210 bovine blood samples of Thai cattle from six farms by hematocrit centrifuge technique (HCT) revealed 14 samples with trypanosomes morphologically compatible to T. theileri. Additional animals were positive for T. theileri by PCR based on the Cathepsin L-like sequence (TthCATL-PCR) despite negative by HCT, indicating cryptic infections. Results revealed a prevalence of 26 ± 15% (95% CI) of T. theileri infection. Additionally, 12 samples positive for T. theileri were detected in cattle from other 11 farms. From a total of 30 blood samples positive by HCT and/or PCR from 17 farms, seven were characterized to evaluate the genetic polymorphism of T. theileri through sequence analysis of PCR-amplified CATL DNA sequences. All CATL sequences of T. theileri from Thai cattle clustered with sequences of the previously described phylogenetic lineages TthI and TthII, supporting only two major lineages of T. theileri in cattle around the world. However, 11 of the 29 CATL sequences analyzed showed to be different, disclosing an unexpectedly large polymorphic genetic repertoire, with multiple genotypes of T. theileri not previously described in other countries circulating in Thai cattle. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/566037/1/document_566037.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.vetpar.2011.03.017 10.1016/j.vetpar.2011.03.017 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=201987 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2011.03.017 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.vetpar.2011.03.017 |
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U30 - Méthodes de recherche L73 - Maladies des animaux Trypanosoma theileri variation génétique trypanosomose diagnostic peptidase gène PCR bovin http://aims.fao.org/aos/agrovoc/c_27407 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_5690 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_7701 U30 - Méthodes de recherche L73 - Maladies des animaux Trypanosoma theileri variation génétique trypanosomose diagnostic peptidase gène PCR bovin http://aims.fao.org/aos/agrovoc/c_27407 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_5690 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_7701 |
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U30 - Méthodes de recherche L73 - Maladies des animaux Trypanosoma theileri variation génétique trypanosomose diagnostic peptidase gène PCR bovin http://aims.fao.org/aos/agrovoc/c_27407 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_5690 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_7701 U30 - Méthodes de recherche L73 - Maladies des animaux Trypanosoma theileri variation génétique trypanosomose diagnostic peptidase gène PCR bovin http://aims.fao.org/aos/agrovoc/c_27407 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_5690 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_7701 Garcia, Herakles A. Kamyingkird, Ketsarin Rodrigues, Adriana C. Jittapalapong, Sathaporn Teixeira, Marta M.G. Desquesnes, Marc High genetic diversity in field isolates of Trypanosoma theileri assessed by analysis of cathepsin L-like sequences disclosed multiple and new genotypes infecting cattle in Thailand |
description |
In this study, we describe the first survey in Thailand of Trypanosoma theileri, a widespread and prevalent parasite of cattle that is transmitted by tabanid flies. Investigation of 210 bovine blood samples of Thai cattle from six farms by hematocrit centrifuge technique (HCT) revealed 14 samples with trypanosomes morphologically compatible to T. theileri. Additional animals were positive for T. theileri by PCR based on the Cathepsin L-like sequence (TthCATL-PCR) despite negative by HCT, indicating cryptic infections. Results revealed a prevalence of 26 ± 15% (95% CI) of T. theileri infection. Additionally, 12 samples positive for T. theileri were detected in cattle from other 11 farms. From a total of 30 blood samples positive by HCT and/or PCR from 17 farms, seven were characterized to evaluate the genetic polymorphism of T. theileri through sequence analysis of PCR-amplified CATL DNA sequences. All CATL sequences of T. theileri from Thai cattle clustered with sequences of the previously described phylogenetic lineages TthI and TthII, supporting only two major lineages of T. theileri in cattle around the world. However, 11 of the 29 CATL sequences analyzed showed to be different, disclosing an unexpectedly large polymorphic genetic repertoire, with multiple genotypes of T. theileri not previously described in other countries circulating in Thai cattle. |
format |
article |
topic_facet |
U30 - Méthodes de recherche L73 - Maladies des animaux Trypanosoma theileri variation génétique trypanosomose diagnostic peptidase gène PCR bovin http://aims.fao.org/aos/agrovoc/c_27407 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_5690 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_7701 |
author |
Garcia, Herakles A. Kamyingkird, Ketsarin Rodrigues, Adriana C. Jittapalapong, Sathaporn Teixeira, Marta M.G. Desquesnes, Marc |
author_facet |
Garcia, Herakles A. Kamyingkird, Ketsarin Rodrigues, Adriana C. Jittapalapong, Sathaporn Teixeira, Marta M.G. Desquesnes, Marc |
author_sort |
Garcia, Herakles A. |
title |
High genetic diversity in field isolates of Trypanosoma theileri assessed by analysis of cathepsin L-like sequences disclosed multiple and new genotypes infecting cattle in Thailand |
title_short |
High genetic diversity in field isolates of Trypanosoma theileri assessed by analysis of cathepsin L-like sequences disclosed multiple and new genotypes infecting cattle in Thailand |
title_full |
High genetic diversity in field isolates of Trypanosoma theileri assessed by analysis of cathepsin L-like sequences disclosed multiple and new genotypes infecting cattle in Thailand |
title_fullStr |
High genetic diversity in field isolates of Trypanosoma theileri assessed by analysis of cathepsin L-like sequences disclosed multiple and new genotypes infecting cattle in Thailand |
title_full_unstemmed |
High genetic diversity in field isolates of Trypanosoma theileri assessed by analysis of cathepsin L-like sequences disclosed multiple and new genotypes infecting cattle in Thailand |
title_sort |
high genetic diversity in field isolates of trypanosoma theileri assessed by analysis of cathepsin l-like sequences disclosed multiple and new genotypes infecting cattle in thailand |
url |
http://agritrop.cirad.fr/566037/ http://agritrop.cirad.fr/566037/1/document_566037.pdf |
work_keys_str_mv |
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