Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae)
Nine microsatellite markers were isolated from unfed larvae of Ixodes ricinus and were tested on two populations of nymphs collected on roe deer (N=21) and birds (N=39) in a French suburban forest. All markers were polymorphic, with limited evidence for deviations from linkage equilibrium. In accordance with previous markers developed for this species, we found large heterozygote deficits for six of the nine loci. Deficits were of the same order of magnitude within a tick infrapopulation, suggesting that population-level estimates were not due to a Wahlund effect among individual hosts, but more likely to technical problems (i.e., null alleles due to mutations in the flanking regions of the microsatellites). Although micro-geographic substructure (e.g., homogamy within infrapopulations) can not be ruled out, it is possible that null alleles could be an inherent problem associated with this tick species and specific genome-level studies are called for. Despite the possible presence of null alleles, the precision of population genetic estimates was improved by the addition of the newly-developed markers making them a useful addition for studying the population ecology of I. ricinus.
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Subjects: | L72 - Organismes nuisibles des animaux, L73 - Maladies des animaux, Ixodes ricinus, microsatellite, marqueur génétique, polymorphisme génétique, parasite, génétique des populations, maladie transmissible par tiques, chevreuil, oiseau, http://aims.fao.org/aos/agrovoc/c_30962, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_24031, http://aims.fao.org/aos/agrovoc/c_5574, http://aims.fao.org/aos/agrovoc/c_34326, http://aims.fao.org/aos/agrovoc/c_24908, http://aims.fao.org/aos/agrovoc/c_24107, http://aims.fao.org/aos/agrovoc/c_935, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/565867/ http://agritrop.cirad.fr/565867/1/document_565867.pdf |
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dig-cirad-fr-5658672024-01-28T20:46:50Z http://agritrop.cirad.fr/565867/ http://agritrop.cirad.fr/565867/ Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae). Noel Valérie, Leger Elsa, Gómez-Díaz Elena, Risterucci Ange-Marie, McCoy Karen D.. 2012. Acarologia, 52 (2) : 123-128.https://doi.org/10.1051/acarologia/20122041 <https://doi.org/10.1051/acarologia/20122041> Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae) Noel, Valérie Leger, Elsa Gómez-Díaz, Elena Risterucci, Ange-Marie McCoy, Karen D. eng 2012 Acarologia L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Ixodes ricinus microsatellite marqueur génétique polymorphisme génétique parasite génétique des populations maladie transmissible par tiques chevreuil oiseau http://aims.fao.org/aos/agrovoc/c_30962 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_24107 http://aims.fao.org/aos/agrovoc/c_935 France http://aims.fao.org/aos/agrovoc/c_3081 Nine microsatellite markers were isolated from unfed larvae of Ixodes ricinus and were tested on two populations of nymphs collected on roe deer (N=21) and birds (N=39) in a French suburban forest. All markers were polymorphic, with limited evidence for deviations from linkage equilibrium. In accordance with previous markers developed for this species, we found large heterozygote deficits for six of the nine loci. Deficits were of the same order of magnitude within a tick infrapopulation, suggesting that population-level estimates were not due to a Wahlund effect among individual hosts, but more likely to technical problems (i.e., null alleles due to mutations in the flanking regions of the microsatellites). Although micro-geographic substructure (e.g., homogamy within infrapopulations) can not be ruled out, it is possible that null alleles could be an inherent problem associated with this tick species and specific genome-level studies are called for. Despite the possible presence of null alleles, the precision of population genetic estimates was improved by the addition of the newly-developed markers making them a useful addition for studying the population ecology of I. ricinus. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/565867/1/document_565867.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1051/acarologia/20122041 10.1051/acarologia/20122041 info:eu-repo/semantics/altIdentifier/doi/10.1051/acarologia/20122041 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1051/acarologia/20122041 |
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L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Ixodes ricinus microsatellite marqueur génétique polymorphisme génétique parasite génétique des populations maladie transmissible par tiques chevreuil oiseau http://aims.fao.org/aos/agrovoc/c_30962 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_24107 http://aims.fao.org/aos/agrovoc/c_935 http://aims.fao.org/aos/agrovoc/c_3081 L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Ixodes ricinus microsatellite marqueur génétique polymorphisme génétique parasite génétique des populations maladie transmissible par tiques chevreuil oiseau http://aims.fao.org/aos/agrovoc/c_30962 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_24107 http://aims.fao.org/aos/agrovoc/c_935 http://aims.fao.org/aos/agrovoc/c_3081 |
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L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Ixodes ricinus microsatellite marqueur génétique polymorphisme génétique parasite génétique des populations maladie transmissible par tiques chevreuil oiseau http://aims.fao.org/aos/agrovoc/c_30962 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_24107 http://aims.fao.org/aos/agrovoc/c_935 http://aims.fao.org/aos/agrovoc/c_3081 L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Ixodes ricinus microsatellite marqueur génétique polymorphisme génétique parasite génétique des populations maladie transmissible par tiques chevreuil oiseau http://aims.fao.org/aos/agrovoc/c_30962 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_24107 http://aims.fao.org/aos/agrovoc/c_935 http://aims.fao.org/aos/agrovoc/c_3081 Noel, Valérie Leger, Elsa Gómez-Díaz, Elena Risterucci, Ange-Marie McCoy, Karen D. Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae) |
description |
Nine microsatellite markers were isolated from unfed larvae of Ixodes ricinus and were tested on two populations of nymphs collected on roe deer (N=21) and birds (N=39) in a French suburban forest. All markers were polymorphic, with limited evidence for deviations from linkage equilibrium. In accordance with previous markers developed for this species, we found large heterozygote deficits for six of the nine loci. Deficits were of the same order of magnitude within a tick infrapopulation, suggesting that population-level estimates were not due to a Wahlund effect among individual hosts, but more likely to technical problems (i.e., null alleles due to mutations in the flanking regions of the microsatellites). Although micro-geographic substructure (e.g., homogamy within infrapopulations) can not be ruled out, it is possible that null alleles could be an inherent problem associated with this tick species and specific genome-level studies are called for. Despite the possible presence of null alleles, the precision of population genetic estimates was improved by the addition of the newly-developed markers making them a useful addition for studying the population ecology of I. ricinus. |
format |
article |
topic_facet |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Ixodes ricinus microsatellite marqueur génétique polymorphisme génétique parasite génétique des populations maladie transmissible par tiques chevreuil oiseau http://aims.fao.org/aos/agrovoc/c_30962 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_24107 http://aims.fao.org/aos/agrovoc/c_935 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Noel, Valérie Leger, Elsa Gómez-Díaz, Elena Risterucci, Ange-Marie McCoy, Karen D. |
author_facet |
Noel, Valérie Leger, Elsa Gómez-Díaz, Elena Risterucci, Ange-Marie McCoy, Karen D. |
author_sort |
Noel, Valérie |
title |
Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae) |
title_short |
Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae) |
title_full |
Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae) |
title_fullStr |
Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae) |
title_full_unstemmed |
Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae) |
title_sort |
isolation and characterization of new polymorphic microsatellite markers for the tick ixodes ricinus (acari, ixodidae) |
url |
http://agritrop.cirad.fr/565867/ http://agritrop.cirad.fr/565867/1/document_565867.pdf |
work_keys_str_mv |
AT noelvalerie isolationandcharacterizationofnewpolymorphicmicrosatellitemarkersforthetickixodesricinusacariixodidae AT legerelsa isolationandcharacterizationofnewpolymorphicmicrosatellitemarkersforthetickixodesricinusacariixodidae AT gomezdiazelena isolationandcharacterizationofnewpolymorphicmicrosatellitemarkersforthetickixodesricinusacariixodidae AT risterucciangemarie isolationandcharacterizationofnewpolymorphicmicrosatellitemarkersforthetickixodesricinusacariixodidae AT mccoykarend isolationandcharacterizationofnewpolymorphicmicrosatellitemarkersforthetickixodesricinusacariixodidae |
_version_ |
1792498296217927680 |