Genetic diversity and phylogeography of cassava mosaic geminiviruses in Madagascar. [S02-12]
Cassava mosaic disease (CMD) caused by cassava mosaic geminiviruses (CMGs, Geminiviridae) is a major threat on cassava production throughout Africa. In Madagascar, severe symptoms with high prevalence of CMD were observed. From 700 mostly symptomatic cassava leaves, we undertook molecular characterization of the CMGs involved. The PCR diagnosis confirmed the presence of CMGs in Madagascar and six species were detected with the frequent occurrence of mixed infections. Based on rolling circle amplification, cloning and sequencing, we obtained full genome sequences of 281 DNA-A and 119 DNA-B of CMGs. DNA-A sequences were most closely related to those of African cassava mosaic virus (ACMV), South African cassava mosaic virus (SACMV), East African cassava mosaic virus (EACMV), East African cassava mosaic Cameroon virus (EACMCV), East African cassava mosaic Kenya virus (EACMKV) and a new species named cassava mosaic Madagascar virus (CMMGV). DNAB sequences were most closely related to those of ACMV, EACMKV, EACMCV and CMMGV. Phylogenetic reconstruction allowed to assess the geographical structure of CMG populations and to determine the recent history of CMG migrations in Madagascar. Taken together, our results point to Madagascar as a potential major center of begomovirus diversity.
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dig-cirad-fr-5655932024-05-02T10:19:42Z http://agritrop.cirad.fr/565593/ http://agritrop.cirad.fr/565593/ Genetic diversity and phylogeography of cassava mosaic geminiviruses in Madagascar. [S02-12]. Harimalala Mireille Aurélie, De Bruyn Alexandre, Lefeuvre Pierre, Villemot Julie, Hoareau Murielle, Andrianjaka Alice, Ranomenjanahary Sahondramalala, Reynaud Bernard, Lett Jean-Michel. 2012. In : Second Scientific Conference of the Global Cassava Patnership the 21st Century (GCP21-II), Kampala, Uganda, June 18-22, 2012. s.l. : s.n., Résumé, 1 p. Scientific Conference of the Global Cassava Partnership for the 21st Century. 2, Kampala, Ouganda, 18 Juin 2012/22 Juin 2012.http://www.gcp21.org/gcp21-ii/8.GCP21-II.Abstracts/S2%20CMD%20&%20CBB/S2%20Oral%20Presentations/S02-12_Harimalala.pdf <http://www.gcp21.org/gcp21-ii/8.GCP21-II.Abstracts/S2%20CMD%20&%20CBB/S2%20Oral%20Presentations/S02-12_Harimalala.pdf> Genetic diversity and phylogeography of cassava mosaic geminiviruses in Madagascar. [S02-12] Harimalala, Mireille Aurélie De Bruyn, Alexandre Lefeuvre, Pierre Villemot, Julie Hoareau, Murielle Andrianjaka, Alice Ranomenjanahary, Sahondramalala Reynaud, Bernard Lett, Jean-Michel eng 2012 s.n. Second Scientific Conference of the Global Cassava Patnership the 21st Century (GCP21-II), Kampala, Uganda, June 18-22, 2012 H20 - Maladies des plantes Cassava mosaic disease (CMD) caused by cassava mosaic geminiviruses (CMGs, Geminiviridae) is a major threat on cassava production throughout Africa. In Madagascar, severe symptoms with high prevalence of CMD were observed. From 700 mostly symptomatic cassava leaves, we undertook molecular characterization of the CMGs involved. The PCR diagnosis confirmed the presence of CMGs in Madagascar and six species were detected with the frequent occurrence of mixed infections. Based on rolling circle amplification, cloning and sequencing, we obtained full genome sequences of 281 DNA-A and 119 DNA-B of CMGs. DNA-A sequences were most closely related to those of African cassava mosaic virus (ACMV), South African cassava mosaic virus (SACMV), East African cassava mosaic virus (EACMV), East African cassava mosaic Cameroon virus (EACMCV), East African cassava mosaic Kenya virus (EACMKV) and a new species named cassava mosaic Madagascar virus (CMMGV). DNAB sequences were most closely related to those of ACMV, EACMKV, EACMCV and CMMGV. Phylogenetic reconstruction allowed to assess the geographical structure of CMG populations and to determine the recent history of CMG migrations in Madagascar. Taken together, our results point to Madagascar as a potential major center of begomovirus diversity. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/565593/1/document_565593.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://www.gcp21.org/gcp21-ii/8.GCP21-II.Abstracts/S2%20CMD%20&%20CBB/S2%20Oral%20Presentations/S02-12_Harimalala.pdf info:eu-repo/semantics/altIdentifier/purl/http://www.gcp21.org/gcp21-ii/8.GCP21-II.Abstracts/S2%20CMD%20&%20CBB/S2%20Oral%20Presentations/S02-12_Harimalala.pdf |
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H20 - Maladies des plantes H20 - Maladies des plantes Harimalala, Mireille Aurélie De Bruyn, Alexandre Lefeuvre, Pierre Villemot, Julie Hoareau, Murielle Andrianjaka, Alice Ranomenjanahary, Sahondramalala Reynaud, Bernard Lett, Jean-Michel Genetic diversity and phylogeography of cassava mosaic geminiviruses in Madagascar. [S02-12] |
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Cassava mosaic disease (CMD) caused by cassava mosaic geminiviruses (CMGs, Geminiviridae) is a major threat on cassava production throughout Africa. In Madagascar, severe symptoms with high prevalence of CMD were observed. From 700 mostly symptomatic cassava leaves, we undertook molecular characterization of the CMGs involved. The PCR diagnosis confirmed the presence of CMGs in Madagascar and six species were detected with the frequent occurrence of mixed infections. Based on rolling circle amplification, cloning and sequencing, we obtained full genome sequences of 281 DNA-A and 119 DNA-B of CMGs. DNA-A sequences were most closely related to those of African cassava mosaic virus (ACMV), South African cassava mosaic virus (SACMV), East African cassava mosaic virus (EACMV), East African cassava mosaic Cameroon virus (EACMCV), East African cassava mosaic Kenya virus (EACMKV) and a new species named cassava mosaic Madagascar virus (CMMGV). DNAB sequences were most closely related to those of ACMV, EACMKV, EACMCV and CMMGV. Phylogenetic reconstruction allowed to assess the geographical structure of CMG populations and to determine the recent history of CMG migrations in Madagascar. Taken together, our results point to Madagascar as a potential major center of begomovirus diversity. |
format |
conference_item |
topic_facet |
H20 - Maladies des plantes |
author |
Harimalala, Mireille Aurélie De Bruyn, Alexandre Lefeuvre, Pierre Villemot, Julie Hoareau, Murielle Andrianjaka, Alice Ranomenjanahary, Sahondramalala Reynaud, Bernard Lett, Jean-Michel |
author_facet |
Harimalala, Mireille Aurélie De Bruyn, Alexandre Lefeuvre, Pierre Villemot, Julie Hoareau, Murielle Andrianjaka, Alice Ranomenjanahary, Sahondramalala Reynaud, Bernard Lett, Jean-Michel |
author_sort |
Harimalala, Mireille Aurélie |
title |
Genetic diversity and phylogeography of cassava mosaic geminiviruses in Madagascar. [S02-12] |
title_short |
Genetic diversity and phylogeography of cassava mosaic geminiviruses in Madagascar. [S02-12] |
title_full |
Genetic diversity and phylogeography of cassava mosaic geminiviruses in Madagascar. [S02-12] |
title_fullStr |
Genetic diversity and phylogeography of cassava mosaic geminiviruses in Madagascar. [S02-12] |
title_full_unstemmed |
Genetic diversity and phylogeography of cassava mosaic geminiviruses in Madagascar. [S02-12] |
title_sort |
genetic diversity and phylogeography of cassava mosaic geminiviruses in madagascar. [s02-12] |
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s.n. |
url |
http://agritrop.cirad.fr/565593/ http://agritrop.cirad.fr/565593/1/document_565593.pdf |
work_keys_str_mv |
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