Catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease
Variations in Cavendish bananas susceptibility to crown rot disease have been observed (Lassois et al., 2010a), but the molecular mechanisms underlying these quantitative host-pathogen relationships were still unknown. The present study was designed to compare gene expression between bananas (Musa acuminata, AAA, 'Grande-Naine') showing a high post-harvest susceptibility (S+) and bananas showing a low post-harvest susceptibility (S-) to crown rot disease. This comparison was performed between crowns (S+ and S-) collected one hour before standardized artificial inoculations with Colletotrichum musae. Fruit susceptibility was evaluated through lesion size on the crown 13 days later. Gene expression comparisons were performed with the cDNA-AFLP technique (Lassois et al., 2009). This revealed that a gene showing a strong homology with a dopamine-?-monooxygenase (DoH) is differently expressed between S+ and S- (Lassois et al., 2011). Furthermore, semi-quantitative real-time RT-PCR analyses between S+ and S- were applied to confirm the differential expression results for DoH obtained by cDNA-AFLP. Two biological replicates were tested. These semi-quantitative analyses were performed not only on tissues collected one hour before C. musae inoculation but also on crown tissues collected 13 days after inoculation. The real-time RT-PCR confirmed that DoH was upregulated in the S- tissues collected at harvest, just before C. musae inoculation. This gene was also highly upregulated in the S- tissues collected 13 days after crown inoculation. Similar results were obtained for both biological replicates. Our results suggest that cathecolamines could play a role in banana defense mechanisms to crown rot disease.
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dig-cirad-fr-5651042024-01-28T20:39:14Z http://agritrop.cirad.fr/565104/ http://agritrop.cirad.fr/565104/ Catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease. Lassois Ludivine, De Clerck Caroline, Frettinger Patrick, De Lapeyre de Bellaire Luc, Lepoivre Philippe, Jijakli M. Haïssam. 2011. Communications in Agricultural and Applied Biological Sciences, 76 (4) : 591-602. International Symposium on Crop Protection. 63, Ghent, Belgique, 24 Mai 2011. Catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease Lassois, Ludivine De Clerck, Caroline Frettinger, Patrick De Lapeyre de Bellaire, Luc Lepoivre, Philippe Jijakli, M. Haïssam eng 2011 Communications in Agricultural and Applied Biological Sciences H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa pourriture maladie postrécolte Colletotrichum catécholamine biosynthèse mécanisme de défense résistance génétique expression des gènes http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_1386 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 Cameroun http://aims.fao.org/aos/agrovoc/c_1229 Variations in Cavendish bananas susceptibility to crown rot disease have been observed (Lassois et al., 2010a), but the molecular mechanisms underlying these quantitative host-pathogen relationships were still unknown. The present study was designed to compare gene expression between bananas (Musa acuminata, AAA, 'Grande-Naine') showing a high post-harvest susceptibility (S+) and bananas showing a low post-harvest susceptibility (S-) to crown rot disease. This comparison was performed between crowns (S+ and S-) collected one hour before standardized artificial inoculations with Colletotrichum musae. Fruit susceptibility was evaluated through lesion size on the crown 13 days later. Gene expression comparisons were performed with the cDNA-AFLP technique (Lassois et al., 2009). This revealed that a gene showing a strong homology with a dopamine-?-monooxygenase (DoH) is differently expressed between S+ and S- (Lassois et al., 2011). Furthermore, semi-quantitative real-time RT-PCR analyses between S+ and S- were applied to confirm the differential expression results for DoH obtained by cDNA-AFLP. Two biological replicates were tested. These semi-quantitative analyses were performed not only on tissues collected one hour before C. musae inoculation but also on crown tissues collected 13 days after inoculation. The real-time RT-PCR confirmed that DoH was upregulated in the S- tissues collected at harvest, just before C. musae inoculation. This gene was also highly upregulated in the S- tissues collected 13 days after crown inoculation. Similar results were obtained for both biological replicates. Our results suggest that cathecolamines could play a role in banana defense mechanisms to crown rot disease. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/565104/1/document_565104.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa pourriture maladie postrécolte Colletotrichum catécholamine biosynthèse mécanisme de défense résistance génétique expression des gènes http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_1386 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1229 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa pourriture maladie postrécolte Colletotrichum catécholamine biosynthèse mécanisme de défense résistance génétique expression des gènes http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_1386 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1229 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa pourriture maladie postrécolte Colletotrichum catécholamine biosynthèse mécanisme de défense résistance génétique expression des gènes http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_1386 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1229 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa pourriture maladie postrécolte Colletotrichum catécholamine biosynthèse mécanisme de défense résistance génétique expression des gènes http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_1386 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1229 Lassois, Ludivine De Clerck, Caroline Frettinger, Patrick De Lapeyre de Bellaire, Luc Lepoivre, Philippe Jijakli, M. Haïssam Catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease |
description |
Variations in Cavendish bananas susceptibility to crown rot disease have been observed (Lassois et al., 2010a), but the molecular mechanisms underlying these quantitative host-pathogen relationships were still unknown. The present study was designed to compare gene expression between bananas (Musa acuminata, AAA, 'Grande-Naine') showing a high post-harvest susceptibility (S+) and bananas showing a low post-harvest susceptibility (S-) to crown rot disease. This comparison was performed between crowns (S+ and S-) collected one hour before standardized artificial inoculations with Colletotrichum musae. Fruit susceptibility was evaluated through lesion size on the crown 13 days later. Gene expression comparisons were performed with the cDNA-AFLP technique (Lassois et al., 2009). This revealed that a gene showing a strong homology with a dopamine-?-monooxygenase (DoH) is differently expressed between S+ and S- (Lassois et al., 2011). Furthermore, semi-quantitative real-time RT-PCR analyses between S+ and S- were applied to confirm the differential expression results for DoH obtained by cDNA-AFLP. Two biological replicates were tested. These semi-quantitative analyses were performed not only on tissues collected one hour before C. musae inoculation but also on crown tissues collected 13 days after inoculation. The real-time RT-PCR confirmed that DoH was upregulated in the S- tissues collected at harvest, just before C. musae inoculation. This gene was also highly upregulated in the S- tissues collected 13 days after crown inoculation. Similar results were obtained for both biological replicates. Our results suggest that cathecolamines could play a role in banana defense mechanisms to crown rot disease. |
format |
article |
topic_facet |
H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa pourriture maladie postrécolte Colletotrichum catécholamine biosynthèse mécanisme de défense résistance génétique expression des gènes http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_1386 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1229 |
author |
Lassois, Ludivine De Clerck, Caroline Frettinger, Patrick De Lapeyre de Bellaire, Luc Lepoivre, Philippe Jijakli, M. Haïssam |
author_facet |
Lassois, Ludivine De Clerck, Caroline Frettinger, Patrick De Lapeyre de Bellaire, Luc Lepoivre, Philippe Jijakli, M. Haïssam |
author_sort |
Lassois, Ludivine |
title |
Catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease |
title_short |
Catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease |
title_full |
Catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease |
title_fullStr |
Catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease |
title_full_unstemmed |
Catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease |
title_sort |
catecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease |
url |
http://agritrop.cirad.fr/565104/ http://agritrop.cirad.fr/565104/1/document_565104.pdf |
work_keys_str_mv |
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1792498251567464448 |