Development of nuclear microsatellite markers for the fonio, Digitaria exilis (Poaceae), an understudied West African cereal
Premise of the study: We developed nuclear microsatellite primers to explore the genetic diversity, population genetic structure, and evolutionary history of the fonio (Digitaria exilis), an understudied cereal cultivated in West Africa. o Methods and Results: We used a microsatellite-enriched library approach to isolate and characterize 38 nuclear primer pairs (31 di-, five tri-, and two tetranucleotide repeats), of which 21 were polymorphic and exhibited a clear pattern in 36 accessions from West Africa. The number of alleles per locus ranged from two to 22, with a mean of 4.71, and expected heterozygosity ranged from 0.03 to 0.93. o Conclusions: The developed set of 21 polymorphic SSR markers will provide tools for population and evolutionary genetics studies of the cultivated fonio.
| Main Authors: | , , , , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F30 - Génétique et amélioration des plantes, F70 - Taxonomie végétale et phytogéographie, Digitaria exilis, microsatellite, variation génétique, http://aims.fao.org/aos/agrovoc/c_2280, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_8355, http://aims.fao.org/aos/agrovoc/c_3423, |
| Online Access: | http://agritrop.cirad.fr/563603/ http://agritrop.cirad.fr/563603/1/document_563603.pdf |
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