Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso
Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD.
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Language: | eng |
Subjects: | H20 - Maladies des plantes, Abelmoschus esculentus, virus des végétaux, espèce nouvelle, phylogénie, adn, séquence d'adn, recombinaison, taxonomie, virologie, begomovirus, http://aims.fao.org/aos/agrovoc/c_8557, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_15733, http://aims.fao.org/aos/agrovoc/c_13325, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_27812, http://aims.fao.org/aos/agrovoc/c_6475, http://aims.fao.org/aos/agrovoc/c_7631, http://aims.fao.org/aos/agrovoc/c_8259, http://aims.fao.org/aos/agrovoc/c_61d49fca, http://aims.fao.org/aos/agrovoc/c_8081, |
Online Access: | http://agritrop.cirad.fr/563503/ http://agritrop.cirad.fr/563503/1/document_563503.pdf |
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dig-cirad-fr-5635032024-01-28T20:13:03Z http://agritrop.cirad.fr/563503/ http://agritrop.cirad.fr/563503/ Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso. Tiendrebeogo Fidèle, Lefeuvre Pierre, Hoareau Murielle, Villemot Julie, Konaté Gnissa, Traoré Alfred S., Barro Nicolas, Traore Valentin S.E., Reynaud Bernard, Traoré Oumar, Lett Jean-Michel. 2010. Virology Journal, 7, 10 p.https://doi.org/10.1186/1743-422X-7-48 <https://doi.org/10.1186/1743-422X-7-48> Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso Tiendrebeogo, Fidèle Lefeuvre, Pierre Hoareau, Murielle Villemot, Julie Konaté, Gnissa Traoré, Alfred S. Barro, Nicolas Traore, Valentin S.E. Reynaud, Bernard Traoré, Oumar Lett, Jean-Michel eng 2010 Virology Journal H20 - Maladies des plantes Abelmoschus esculentus virus des végétaux espèce nouvelle phylogénie adn séquence d'adn recombinaison taxonomie virologie begomovirus http://aims.fao.org/aos/agrovoc/c_8557 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15733 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_61d49fca Burkina Faso http://aims.fao.org/aos/agrovoc/c_8081 Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/563503/1/document_563503.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1186/1743-422X-7-48 10.1186/1743-422X-7-48 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=208489 info:eu-repo/semantics/altIdentifier/doi/10.1186/1743-422X-7-48 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/1743-422X-7-48 |
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H20 - Maladies des plantes Abelmoschus esculentus virus des végétaux espèce nouvelle phylogénie adn séquence d'adn recombinaison taxonomie virologie begomovirus http://aims.fao.org/aos/agrovoc/c_8557 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15733 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_8081 H20 - Maladies des plantes Abelmoschus esculentus virus des végétaux espèce nouvelle phylogénie adn séquence d'adn recombinaison taxonomie virologie begomovirus http://aims.fao.org/aos/agrovoc/c_8557 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15733 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_8081 |
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H20 - Maladies des plantes Abelmoschus esculentus virus des végétaux espèce nouvelle phylogénie adn séquence d'adn recombinaison taxonomie virologie begomovirus http://aims.fao.org/aos/agrovoc/c_8557 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15733 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_8081 H20 - Maladies des plantes Abelmoschus esculentus virus des végétaux espèce nouvelle phylogénie adn séquence d'adn recombinaison taxonomie virologie begomovirus http://aims.fao.org/aos/agrovoc/c_8557 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15733 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_8081 Tiendrebeogo, Fidèle Lefeuvre, Pierre Hoareau, Murielle Villemot, Julie Konaté, Gnissa Traoré, Alfred S. Barro, Nicolas Traore, Valentin S.E. Reynaud, Bernard Traoré, Oumar Lett, Jean-Michel Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
description |
Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD. |
format |
article |
topic_facet |
H20 - Maladies des plantes Abelmoschus esculentus virus des végétaux espèce nouvelle phylogénie adn séquence d'adn recombinaison taxonomie virologie begomovirus http://aims.fao.org/aos/agrovoc/c_8557 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15733 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_6475 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_8081 |
author |
Tiendrebeogo, Fidèle Lefeuvre, Pierre Hoareau, Murielle Villemot, Julie Konaté, Gnissa Traoré, Alfred S. Barro, Nicolas Traore, Valentin S.E. Reynaud, Bernard Traoré, Oumar Lett, Jean-Michel |
author_facet |
Tiendrebeogo, Fidèle Lefeuvre, Pierre Hoareau, Murielle Villemot, Julie Konaté, Gnissa Traoré, Alfred S. Barro, Nicolas Traore, Valentin S.E. Reynaud, Bernard Traoré, Oumar Lett, Jean-Michel |
author_sort |
Tiendrebeogo, Fidèle |
title |
Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title_short |
Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title_full |
Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title_fullStr |
Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title_full_unstemmed |
Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title_sort |
molecular diversity of cotton leaf curl gezira virus isolates and their satellite dnas associated with okra leaf curl disease in burkina faso |
url |
http://agritrop.cirad.fr/563503/ http://agritrop.cirad.fr/563503/1/document_563503.pdf |
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