Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species
Sideroxylon majus (Sapotaceae) is an endangered endemic tree of La Re´union Island that has suffered from human actions. It is present in small and isolated populations that encounter severe difficulties to regenerate. To have powerful tools for population genetic studies, we have isolated and characterized 14 polymorphic microsatellite markers from S. majus. The 14 loci were tested on 57 individuals from 6 populations. The number of alleles per locus varied from 2 to 20, with an average of 11.8. The observed and expected heterozygosity levels ranged from 0.053 to 1.000, and 0.116 to 0.917, respectively. Six of the 14 loci deviated from Hardy-Weinberg equilibrium. These polymorphic microsatellite markers constitute new tools to study the genetic diversity and spatial genetic structure of S. majus. The cross-species amplifications indicate that most of these loci can be used to investigate population genetic structure in S. grandiflorum, S. boutonianum and S. sessiliflorum. These studies will provide useful results for the elaboration of effective conservation strategies.
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Subjects: | F30 - Génétique et amélioration des plantes, F70 - Taxonomie végétale et phytogéographie, P01 - Conservation de la nature et ressources foncières, Sapotaceae, espèce en danger, marqueur génétique, microsatellite, identification, conservation des ressources génétiques, génie génétique, http://aims.fao.org/aos/agrovoc/c_6796, http://aims.fao.org/aos/agrovoc/c_2557, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_37280, http://aims.fao.org/aos/agrovoc/c_15974, http://aims.fao.org/aos/agrovoc/c_6543, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/561334/ http://agritrop.cirad.fr/561334/1/document_561334.pdf |
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dig-cirad-fr-5613342024-01-28T19:33:10Z http://agritrop.cirad.fr/561334/ http://agritrop.cirad.fr/561334/ Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species. Dafreville Stéphanie, Payet Guillaume, Simiand Christophe, Risterucci Ange-Marie, Riviere Expédit, Lebreton Gérard, Humeau Laurence, Strasberg Dominique, Chevallier Marie-Hélène. 2011. Conservation Genetics Resources, 3 (4) : 701-704.https://doi.org/10.1007/s12686-011-9437-0 <https://doi.org/10.1007/s12686-011-9437-0> Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species Dafreville, Stéphanie Payet, Guillaume Simiand, Christophe Risterucci, Ange-Marie Riviere, Expédit Lebreton, Gérard Humeau, Laurence Strasberg, Dominique Chevallier, Marie-Hélène eng 2011 Conservation Genetics Resources F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie P01 - Conservation de la nature et ressources foncières Sapotaceae espèce en danger marqueur génétique microsatellite identification conservation des ressources génétiques génie génétique http://aims.fao.org/aos/agrovoc/c_6796 http://aims.fao.org/aos/agrovoc/c_2557 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37280 http://aims.fao.org/aos/agrovoc/c_15974 La Réunion France http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Sideroxylon majus (Sapotaceae) is an endangered endemic tree of La Re´union Island that has suffered from human actions. It is present in small and isolated populations that encounter severe difficulties to regenerate. To have powerful tools for population genetic studies, we have isolated and characterized 14 polymorphic microsatellite markers from S. majus. The 14 loci were tested on 57 individuals from 6 populations. The number of alleles per locus varied from 2 to 20, with an average of 11.8. The observed and expected heterozygosity levels ranged from 0.053 to 1.000, and 0.116 to 0.917, respectively. Six of the 14 loci deviated from Hardy-Weinberg equilibrium. These polymorphic microsatellite markers constitute new tools to study the genetic diversity and spatial genetic structure of S. majus. The cross-species amplifications indicate that most of these loci can be used to investigate population genetic structure in S. grandiflorum, S. boutonianum and S. sessiliflorum. These studies will provide useful results for the elaboration of effective conservation strategies. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/561334/1/document_561334.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s12686-011-9437-0 10.1007/s12686-011-9437-0 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=212540 info:eu-repo/semantics/altIdentifier/doi/10.1007/s12686-011-9437-0 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s12686-011-9437-0 |
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F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie P01 - Conservation de la nature et ressources foncières Sapotaceae espèce en danger marqueur génétique microsatellite identification conservation des ressources génétiques génie génétique http://aims.fao.org/aos/agrovoc/c_6796 http://aims.fao.org/aos/agrovoc/c_2557 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37280 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie P01 - Conservation de la nature et ressources foncières Sapotaceae espèce en danger marqueur génétique microsatellite identification conservation des ressources génétiques génie génétique http://aims.fao.org/aos/agrovoc/c_6796 http://aims.fao.org/aos/agrovoc/c_2557 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37280 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
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F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie P01 - Conservation de la nature et ressources foncières Sapotaceae espèce en danger marqueur génétique microsatellite identification conservation des ressources génétiques génie génétique http://aims.fao.org/aos/agrovoc/c_6796 http://aims.fao.org/aos/agrovoc/c_2557 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37280 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie P01 - Conservation de la nature et ressources foncières Sapotaceae espèce en danger marqueur génétique microsatellite identification conservation des ressources génétiques génie génétique http://aims.fao.org/aos/agrovoc/c_6796 http://aims.fao.org/aos/agrovoc/c_2557 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37280 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Dafreville, Stéphanie Payet, Guillaume Simiand, Christophe Risterucci, Ange-Marie Riviere, Expédit Lebreton, Gérard Humeau, Laurence Strasberg, Dominique Chevallier, Marie-Hélène Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species |
description |
Sideroxylon majus (Sapotaceae) is an endangered endemic tree of La Re´union Island that has suffered from human actions. It is present in small and isolated populations that encounter severe difficulties to regenerate. To have powerful tools for population genetic studies, we have isolated and characterized 14 polymorphic microsatellite markers from S. majus. The 14 loci were tested on 57 individuals from 6 populations. The number of alleles per locus varied from 2 to 20, with an average of 11.8. The observed and expected heterozygosity levels ranged from 0.053 to 1.000, and 0.116 to 0.917, respectively. Six of the 14 loci deviated from Hardy-Weinberg equilibrium. These polymorphic microsatellite markers constitute new tools to study the genetic diversity and spatial genetic structure of S. majus. The cross-species amplifications indicate that most of these loci can be used to investigate population genetic structure in S. grandiflorum, S. boutonianum and S. sessiliflorum. These studies will provide useful results for the elaboration of effective conservation strategies. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie P01 - Conservation de la nature et ressources foncières Sapotaceae espèce en danger marqueur génétique microsatellite identification conservation des ressources génétiques génie génétique http://aims.fao.org/aos/agrovoc/c_6796 http://aims.fao.org/aos/agrovoc/c_2557 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37280 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Dafreville, Stéphanie Payet, Guillaume Simiand, Christophe Risterucci, Ange-Marie Riviere, Expédit Lebreton, Gérard Humeau, Laurence Strasberg, Dominique Chevallier, Marie-Hélène |
author_facet |
Dafreville, Stéphanie Payet, Guillaume Simiand, Christophe Risterucci, Ange-Marie Riviere, Expédit Lebreton, Gérard Humeau, Laurence Strasberg, Dominique Chevallier, Marie-Hélène |
author_sort |
Dafreville, Stéphanie |
title |
Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species |
title_short |
Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species |
title_full |
Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species |
title_fullStr |
Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species |
title_full_unstemmed |
Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species |
title_sort |
isolation and characterization of microsatellite markers of an endangered tropical tree, sideroxylon majus, and cross-species amplification in other sapotaceae species |
url |
http://agritrop.cirad.fr/561334/ http://agritrop.cirad.fr/561334/1/document_561334.pdf |
work_keys_str_mv |
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1792497919520145408 |