Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification
Supercritical fluids offer environmental advantages over chemical solvents, while providing enhanced separation and chemical selectivity. The use of supercritical fluids for the recovery of products from biomass and the transformation of selected molecules (to add value) was studied. Free fatty acids were bio-catalytically transformed to fatty acid esters using lipase within a supercritical fluid environment. A central composite rotatable design was used to evaluate the influence of operating conditions on the enzymatic esterification process and a response surface equation was optimized to identify the most favourable process conditions for maximum free fatty acid conversion. Based on the model equation the process conditions under which it was predicted a yield of 100% esters could be obtained were: pressure 200 bar, temperature 60 °C, ethanol concentration 2.0 M, enzyme concentration 11 wt.% and time 60 min. Experiments conducted under these conditions gave an ester yield of 94.3% (close to predicted results). The activity per unit mass of biocatalyst was found to be 1585 ?mol/min/gcat. The results support the use of supercritical fluids for process integration.
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Subjects: | Q60 - Traitement des produits agricoles non alimentaires, 000 - Autres thèmes, biomasse, éthanol, extraction au fluide supercritique, dioxyde de carbone, hydrolyse enzymatique, estérase, acide gras, estérification, lipase, http://aims.fao.org/aos/agrovoc/c_926, http://aims.fao.org/aos/agrovoc/c_10677, http://aims.fao.org/aos/agrovoc/c_34343, http://aims.fao.org/aos/agrovoc/c_1302, http://aims.fao.org/aos/agrovoc/c_27512, http://aims.fao.org/aos/agrovoc/c_2670, http://aims.fao.org/aos/agrovoc/c_2818, http://aims.fao.org/aos/agrovoc/c_28361, http://aims.fao.org/aos/agrovoc/c_12194, |
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dig-cirad-fr-5610252024-01-28T19:28:42Z http://agritrop.cirad.fr/561025/ http://agritrop.cirad.fr/561025/ Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification. Baig Muhammad N., Santos R.C.D., Zetzl C., King J., Pioch Daniel, Bowra S.. 2011. Enzyme and Microbial Technology, 49 (4) : 420-426.https://doi.org/10.1016/j.enzmictec.2011.07.004 <https://doi.org/10.1016/j.enzmictec.2011.07.004> Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification Baig, Muhammad N. Santos, R.C.D. Zetzl, C. King, J. Pioch, Daniel Bowra, S. eng 2011 Enzyme and Microbial Technology Q60 - Traitement des produits agricoles non alimentaires 000 - Autres thèmes biomasse éthanol extraction au fluide supercritique dioxyde de carbone hydrolyse enzymatique estérase acide gras estérification lipase http://aims.fao.org/aos/agrovoc/c_926 http://aims.fao.org/aos/agrovoc/c_10677 http://aims.fao.org/aos/agrovoc/c_34343 http://aims.fao.org/aos/agrovoc/c_1302 http://aims.fao.org/aos/agrovoc/c_27512 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_28361 http://aims.fao.org/aos/agrovoc/c_12194 Supercritical fluids offer environmental advantages over chemical solvents, while providing enhanced separation and chemical selectivity. The use of supercritical fluids for the recovery of products from biomass and the transformation of selected molecules (to add value) was studied. Free fatty acids were bio-catalytically transformed to fatty acid esters using lipase within a supercritical fluid environment. A central composite rotatable design was used to evaluate the influence of operating conditions on the enzymatic esterification process and a response surface equation was optimized to identify the most favourable process conditions for maximum free fatty acid conversion. Based on the model equation the process conditions under which it was predicted a yield of 100% esters could be obtained were: pressure 200 bar, temperature 60 °C, ethanol concentration 2.0 M, enzyme concentration 11 wt.% and time 60 min. Experiments conducted under these conditions gave an ester yield of 94.3% (close to predicted results). The activity per unit mass of biocatalyst was found to be 1585 ?mol/min/gcat. The results support the use of supercritical fluids for process integration. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/561025/1/document_561025.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.enzmictec.2011.07.004 10.1016/j.enzmictec.2011.07.004 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2011.07.004 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.enzmictec.2011.07.004 |
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Q60 - Traitement des produits agricoles non alimentaires 000 - Autres thèmes biomasse éthanol extraction au fluide supercritique dioxyde de carbone hydrolyse enzymatique estérase acide gras estérification lipase http://aims.fao.org/aos/agrovoc/c_926 http://aims.fao.org/aos/agrovoc/c_10677 http://aims.fao.org/aos/agrovoc/c_34343 http://aims.fao.org/aos/agrovoc/c_1302 http://aims.fao.org/aos/agrovoc/c_27512 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_28361 http://aims.fao.org/aos/agrovoc/c_12194 Q60 - Traitement des produits agricoles non alimentaires 000 - Autres thèmes biomasse éthanol extraction au fluide supercritique dioxyde de carbone hydrolyse enzymatique estérase acide gras estérification lipase http://aims.fao.org/aos/agrovoc/c_926 http://aims.fao.org/aos/agrovoc/c_10677 http://aims.fao.org/aos/agrovoc/c_34343 http://aims.fao.org/aos/agrovoc/c_1302 http://aims.fao.org/aos/agrovoc/c_27512 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_28361 http://aims.fao.org/aos/agrovoc/c_12194 |
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Q60 - Traitement des produits agricoles non alimentaires 000 - Autres thèmes biomasse éthanol extraction au fluide supercritique dioxyde de carbone hydrolyse enzymatique estérase acide gras estérification lipase http://aims.fao.org/aos/agrovoc/c_926 http://aims.fao.org/aos/agrovoc/c_10677 http://aims.fao.org/aos/agrovoc/c_34343 http://aims.fao.org/aos/agrovoc/c_1302 http://aims.fao.org/aos/agrovoc/c_27512 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_28361 http://aims.fao.org/aos/agrovoc/c_12194 Q60 - Traitement des produits agricoles non alimentaires 000 - Autres thèmes biomasse éthanol extraction au fluide supercritique dioxyde de carbone hydrolyse enzymatique estérase acide gras estérification lipase http://aims.fao.org/aos/agrovoc/c_926 http://aims.fao.org/aos/agrovoc/c_10677 http://aims.fao.org/aos/agrovoc/c_34343 http://aims.fao.org/aos/agrovoc/c_1302 http://aims.fao.org/aos/agrovoc/c_27512 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_28361 http://aims.fao.org/aos/agrovoc/c_12194 Baig, Muhammad N. Santos, R.C.D. Zetzl, C. King, J. Pioch, Daniel Bowra, S. Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification |
description |
Supercritical fluids offer environmental advantages over chemical solvents, while providing enhanced separation and chemical selectivity. The use of supercritical fluids for the recovery of products from biomass and the transformation of selected molecules (to add value) was studied. Free fatty acids were bio-catalytically transformed to fatty acid esters using lipase within a supercritical fluid environment. A central composite rotatable design was used to evaluate the influence of operating conditions on the enzymatic esterification process and a response surface equation was optimized to identify the most favourable process conditions for maximum free fatty acid conversion. Based on the model equation the process conditions under which it was predicted a yield of 100% esters could be obtained were: pressure 200 bar, temperature 60 °C, ethanol concentration 2.0 M, enzyme concentration 11 wt.% and time 60 min. Experiments conducted under these conditions gave an ester yield of 94.3% (close to predicted results). The activity per unit mass of biocatalyst was found to be 1585 ?mol/min/gcat. The results support the use of supercritical fluids for process integration. |
format |
article |
topic_facet |
Q60 - Traitement des produits agricoles non alimentaires 000 - Autres thèmes biomasse éthanol extraction au fluide supercritique dioxyde de carbone hydrolyse enzymatique estérase acide gras estérification lipase http://aims.fao.org/aos/agrovoc/c_926 http://aims.fao.org/aos/agrovoc/c_10677 http://aims.fao.org/aos/agrovoc/c_34343 http://aims.fao.org/aos/agrovoc/c_1302 http://aims.fao.org/aos/agrovoc/c_27512 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_28361 http://aims.fao.org/aos/agrovoc/c_12194 |
author |
Baig, Muhammad N. Santos, R.C.D. Zetzl, C. King, J. Pioch, Daniel Bowra, S. |
author_facet |
Baig, Muhammad N. Santos, R.C.D. Zetzl, C. King, J. Pioch, Daniel Bowra, S. |
author_sort |
Baig, Muhammad N. |
title |
Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification |
title_short |
Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification |
title_full |
Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification |
title_fullStr |
Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification |
title_full_unstemmed |
Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification |
title_sort |
evaluation and modelling the utility of scco2 to support efficient lipase mediated esterification |
url |
http://agritrop.cirad.fr/561025/ http://agritrop.cirad.fr/561025/1/document_561025.pdf |
work_keys_str_mv |
AT baigmuhammadn evaluationandmodellingtheutilityofscco2tosupportefficientlipasemediatedesterification AT santosrcd evaluationandmodellingtheutilityofscco2tosupportefficientlipasemediatedesterification AT zetzlc evaluationandmodellingtheutilityofscco2tosupportefficientlipasemediatedesterification AT kingj evaluationandmodellingtheutilityofscco2tosupportefficientlipasemediatedesterification AT piochdaniel evaluationandmodellingtheutilityofscco2tosupportefficientlipasemediatedesterification AT bowras evaluationandmodellingtheutilityofscco2tosupportefficientlipasemediatedesterification |
_version_ |
1792497894034505728 |