Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability In Citrus (P148)
Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences (BES) of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata , C. maxima , C. medica , C. sinensis , C. aurantium , C. paradisi , C. lemon , C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps.
Main Authors: | , , , , , |
---|---|
Format: | conference_item biblioteca |
Language: | eng |
Published: |
s.n.
|
Subjects: | F30 - Génétique et amélioration des plantes, Citrus, microsatellite, Citrus clementina, http://aims.fao.org/aos/agrovoc/c_1637, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_6ce991dd, |
Online Access: | http://agritrop.cirad.fr/559977/ http://agritrop.cirad.fr/559977/1/document_559977.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-559977 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5599772024-01-28T19:11:56Z http://agritrop.cirad.fr/559977/ http://agritrop.cirad.fr/559977/ Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability In Citrus (P148). Ollitrault Frédérique, Terol Javier F., Pina José Antonio, Navarro Luis, Talon Manuel, Ollitrault Patrick. 2011. In : Abstracts of Plant and Animal Genomes XIXth Conference, San Diego, CA (USA), January 15-19, 2011. s.l. : s.n., Résumé Plant and Animal Genomes Conference. 19, San Diego, États-Unis, 15 Janvier 2011/19 Janvier 2011. Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability In Citrus (P148) Ollitrault, Frédérique Terol, Javier F. Pina, José Antonio Navarro, Luis Talon, Manuel Ollitrault, Patrick eng 2011 s.n. Abstracts of Plant and Animal Genomes XIXth Conference, San Diego, CA (USA), January 15-19, 2011 F30 - Génétique et amélioration des plantes Citrus microsatellite Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_6ce991dd Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences (BES) of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata , C. maxima , C. medica , C. sinensis , C. aurantium , C. paradisi , C. lemon , C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/559977/1/document_559977.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=200206 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
F30 - Génétique et amélioration des plantes Citrus microsatellite Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_6ce991dd F30 - Génétique et amélioration des plantes Citrus microsatellite Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_6ce991dd |
spellingShingle |
F30 - Génétique et amélioration des plantes Citrus microsatellite Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_6ce991dd F30 - Génétique et amélioration des plantes Citrus microsatellite Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_6ce991dd Ollitrault, Frédérique Terol, Javier F. Pina, José Antonio Navarro, Luis Talon, Manuel Ollitrault, Patrick Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability In Citrus (P148) |
description |
Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences (BES) of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata , C. maxima , C. medica , C. sinensis , C. aurantium , C. paradisi , C. lemon , C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps. |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes Citrus microsatellite Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_6ce991dd |
author |
Ollitrault, Frédérique Terol, Javier F. Pina, José Antonio Navarro, Luis Talon, Manuel Ollitrault, Patrick |
author_facet |
Ollitrault, Frédérique Terol, Javier F. Pina, José Antonio Navarro, Luis Talon, Manuel Ollitrault, Patrick |
author_sort |
Ollitrault, Frédérique |
title |
Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability In Citrus (P148) |
title_short |
Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability In Citrus (P148) |
title_full |
Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability In Citrus (P148) |
title_fullStr |
Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability In Citrus (P148) |
title_full_unstemmed |
Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability In Citrus (P148) |
title_sort |
development of ssr markers from citrus clementina (rutaceae) bac end sequences and interspecific transferability in citrus (p148) |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/559977/ http://agritrop.cirad.fr/559977/1/document_559977.pdf |
work_keys_str_mv |
AT ollitraultfrederique developmentofssrmarkersfromcitrusclementinarutaceaebacendsequencesandinterspecifictransferabilityincitrusp148 AT teroljavierf developmentofssrmarkersfromcitrusclementinarutaceaebacendsequencesandinterspecifictransferabilityincitrusp148 AT pinajoseantonio developmentofssrmarkersfromcitrusclementinarutaceaebacendsequencesandinterspecifictransferabilityincitrusp148 AT navarroluis developmentofssrmarkersfromcitrusclementinarutaceaebacendsequencesandinterspecifictransferabilityincitrusp148 AT talonmanuel developmentofssrmarkersfromcitrusclementinarutaceaebacendsequencesandinterspecifictransferabilityincitrusp148 AT ollitraultpatrick developmentofssrmarkersfromcitrusclementinarutaceaebacendsequencesandinterspecifictransferabilityincitrusp148 |
_version_ |
1792497815548592128 |