Exploiting genetic resources for developing rice germplasm with eco-efficient water use
The Cirad/CIAT rice collaborative project focuses on the development and enhancement of rice composite populations (CPs) through recurrent selection (RS) breeding. Our goal is to develop and diffuse improved material for various rainfed ecosystems in Latin America and the Caribbean (LAC). Our breeding strategy is based on the development of broadbase populations; their improvement thought RS and the exploitation of their genetic wealth for line development. From a base population, four CP were derived, some subjected to selections under acid soils conditions (savannas of Colombian Llanos), others selected under the conditions of Bolivian rice agriculture. We used 16 SSR loci to assess the genetic diversity within these populations and estimate genetic differentiation between them. We applied large scale phenotyping method adapted to field conditions to evaluate the response to drought. Through thermographic infra-red technology (IR) we screened S1 progenies extracted from each population which were exposed to a 15-day drought period at flowering stage. Allelic variability measured in the four CP revealed high levels of neutral diversity. The genetic diversity expressed in terms of number of observed alleles per locus (Na) and Shannon diversity index (I), was high within the populations (Na > 3.133, and I = 0.693 to I = 0.800). Significant allelic and genotypic differentiations were found at most loci. A total of six alleles were found with frequency > 5% and unique to a particular population. IR screening showed that the CPs hold favorable alleles for resilience to drought. S1 lines with cool canopy temperatures during the dry period indicated good capacity to maintain transpiration and thus sustained growth under water stress conditions. These lines are potential progenitors to develop a new population with increased eco-efficiency to water use. Besides showing the genetic wealth retained in these CP, our work presents an advance towards the integration of highthroughput phenotyping and use of molecular markers for improving RS breeding strategy. Our objective is not limited to integrating disciplines; the outcomes of the project, both methodologies and germplasm, are shared among our network of rice breeders from LAC.(Texte intégral)
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Subjects: | F30 - Génétique et amélioration des plantes, F62 - Physiologie végétale - Croissance et développement, |
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dig-cirad-fr-5586962021-06-29T16:00:46Z http://agritrop.cirad.fr/558696/ http://agritrop.cirad.fr/558696/ Exploiting genetic resources for developing rice germplasm with eco-efficient water use. Grenier Cécile, Audebert Alain, Sanabria Yamid, Ospina Yolima, Rodriguez Fernando, Châtel Marc. 2010. In : Final program and abstracts of the 2nd International Symposium on Genomics of Plant Genetic Resources, Bologna, Italy, 24-27 April 2010. Rome : Bioversity International, Résumé, 224. International Symposium on Genomics of Plant Genetic Resources. 2, Bologne, Italie, 24 Avril 2010/27 Avril 2010. Researchers Exploiting genetic resources for developing rice germplasm with eco-efficient water use Grenier, Cécile Audebert, Alain Sanabria, Yamid Ospina, Yolima Rodriguez, Fernando Châtel, Marc eng 2010 Bioversity International Final program and abstracts of the 2nd International Symposium on Genomics of Plant Genetic Resources, Bologna, Italy, 24-27 April 2010 F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement The Cirad/CIAT rice collaborative project focuses on the development and enhancement of rice composite populations (CPs) through recurrent selection (RS) breeding. Our goal is to develop and diffuse improved material for various rainfed ecosystems in Latin America and the Caribbean (LAC). Our breeding strategy is based on the development of broadbase populations; their improvement thought RS and the exploitation of their genetic wealth for line development. From a base population, four CP were derived, some subjected to selections under acid soils conditions (savannas of Colombian Llanos), others selected under the conditions of Bolivian rice agriculture. We used 16 SSR loci to assess the genetic diversity within these populations and estimate genetic differentiation between them. We applied large scale phenotyping method adapted to field conditions to evaluate the response to drought. Through thermographic infra-red technology (IR) we screened S1 progenies extracted from each population which were exposed to a 15-day drought period at flowering stage. Allelic variability measured in the four CP revealed high levels of neutral diversity. The genetic diversity expressed in terms of number of observed alleles per locus (Na) and Shannon diversity index (I), was high within the populations (Na > 3.133, and I = 0.693 to I = 0.800). Significant allelic and genotypic differentiations were found at most loci. A total of six alleles were found with frequency > 5% and unique to a particular population. IR screening showed that the CPs hold favorable alleles for resilience to drought. S1 lines with cool canopy temperatures during the dry period indicated good capacity to maintain transpiration and thus sustained growth under water stress conditions. These lines are potential progenitors to develop a new population with increased eco-efficiency to water use. Besides showing the genetic wealth retained in these CP, our work presents an advance towards the integration of highthroughput phenotyping and use of molecular markers for improving RS breeding strategy. Our objective is not limited to integrating disciplines; the outcomes of the project, both methodologies and germplasm, are shared among our network of rice breeders from LAC.(Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/558696/1/document_558696.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=207317 |
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F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement |
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F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Grenier, Cécile Audebert, Alain Sanabria, Yamid Ospina, Yolima Rodriguez, Fernando Châtel, Marc Exploiting genetic resources for developing rice germplasm with eco-efficient water use |
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The Cirad/CIAT rice collaborative project focuses on the development and enhancement of rice composite populations (CPs) through recurrent selection (RS) breeding. Our goal is to develop and diffuse improved material for various rainfed ecosystems in Latin America and the Caribbean (LAC). Our breeding strategy is based on the development of broadbase populations; their improvement thought RS and the exploitation of their genetic wealth for line development. From a base population, four CP were derived, some subjected to selections under acid soils conditions (savannas of Colombian Llanos), others selected under the conditions of Bolivian rice agriculture. We used 16 SSR loci to assess the genetic diversity within these populations and estimate genetic differentiation between them. We applied large scale phenotyping method adapted to field conditions to evaluate the response to drought. Through thermographic infra-red technology (IR) we screened S1 progenies extracted from each population which were exposed to a 15-day drought period at flowering stage. Allelic variability measured in the four CP revealed high levels of neutral diversity. The genetic diversity expressed in terms of number of observed alleles per locus (Na) and Shannon diversity index (I), was high within the populations (Na > 3.133, and I = 0.693 to I = 0.800). Significant allelic and genotypic differentiations were found at most loci. A total of six alleles were found with frequency > 5% and unique to a particular population. IR screening showed that the CPs hold favorable alleles for resilience to drought. S1 lines with cool canopy temperatures during the dry period indicated good capacity to maintain transpiration and thus sustained growth under water stress conditions. These lines are potential progenitors to develop a new population with increased eco-efficiency to water use. Besides showing the genetic wealth retained in these CP, our work presents an advance towards the integration of highthroughput phenotyping and use of molecular markers for improving RS breeding strategy. Our objective is not limited to integrating disciplines; the outcomes of the project, both methodologies and germplasm, are shared among our network of rice breeders from LAC.(Texte intégral) |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement |
author |
Grenier, Cécile Audebert, Alain Sanabria, Yamid Ospina, Yolima Rodriguez, Fernando Châtel, Marc |
author_facet |
Grenier, Cécile Audebert, Alain Sanabria, Yamid Ospina, Yolima Rodriguez, Fernando Châtel, Marc |
author_sort |
Grenier, Cécile |
title |
Exploiting genetic resources for developing rice germplasm with eco-efficient water use |
title_short |
Exploiting genetic resources for developing rice germplasm with eco-efficient water use |
title_full |
Exploiting genetic resources for developing rice germplasm with eco-efficient water use |
title_fullStr |
Exploiting genetic resources for developing rice germplasm with eco-efficient water use |
title_full_unstemmed |
Exploiting genetic resources for developing rice germplasm with eco-efficient water use |
title_sort |
exploiting genetic resources for developing rice germplasm with eco-efficient water use |
publisher |
Bioversity International |
url |
http://agritrop.cirad.fr/558696/ http://agritrop.cirad.fr/558696/1/document_558696.pdf |
work_keys_str_mv |
AT greniercecile exploitinggeneticresourcesfordevelopingricegermplasmwithecoefficientwateruse AT audebertalain exploitinggeneticresourcesfordevelopingricegermplasmwithecoefficientwateruse AT sanabriayamid exploitinggeneticresourcesfordevelopingricegermplasmwithecoefficientwateruse AT ospinayolima exploitinggeneticresourcesfordevelopingricegermplasmwithecoefficientwateruse AT rodriguezfernando exploitinggeneticresourcesfordevelopingricegermplasmwithecoefficientwateruse AT chatelmarc exploitinggeneticresourcesfordevelopingricegermplasmwithecoefficientwateruse |
_version_ |
1758022779181989888 |