Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae)
Premise of the study: Large-scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fi jiensis , a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modifi ed DNA extraction protocol. Methods and Results: Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis. A total of 19 new polymorphic and easy-to-score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. Conclusions: The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis, allowing large-scale analysis of samples from various geographic locations.
Main Authors: | , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, Musa, Mycosphaerella fijiensis, génotype, microsatellite, marqueur génétique, maladie des raies noires, http://aims.fao.org/aos/agrovoc/c_4993, http://aims.fao.org/aos/agrovoc/c_27259, http://aims.fao.org/aos/agrovoc/c_3225, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_36375, |
Online Access: | http://agritrop.cirad.fr/557717/ http://agritrop.cirad.fr/557717/1/document_557717.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-557717 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5577172024-01-28T18:47:34Z http://agritrop.cirad.fr/557717/ http://agritrop.cirad.fr/557717/ Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae). Robert Stéphanie, Rieux Adrien, Argout Xavier, Carlier Jean, Zapater Marie-Françoise. 2010. American Journal of Botany, 97 (12), e130-e132https://doi.org/10.3732/ajb.1000295 <https://doi.org/10.3732/ajb.1000295> Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae) Robert, Stéphanie Rieux, Adrien Argout, Xavier Carlier, Jean Zapater, Marie-Françoise eng 2010 American Journal of Botany H20 - Maladies des plantes Musa Mycosphaerella fijiensis génotype microsatellite marqueur génétique maladie des raies noires http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_27259 http://aims.fao.org/aos/agrovoc/c_3225 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36375 Premise of the study: Large-scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fi jiensis , a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modifi ed DNA extraction protocol. Methods and Results: Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis. A total of 19 new polymorphic and easy-to-score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. Conclusions: The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis, allowing large-scale analysis of samples from various geographic locations. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/557717/1/document_557717.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.3732/ajb.1000295 10.3732/ajb.1000295 info:eu-repo/semantics/altIdentifier/doi/10.3732/ajb.1000295 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3732/ajb.1000295 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H20 - Maladies des plantes Musa Mycosphaerella fijiensis génotype microsatellite marqueur génétique maladie des raies noires http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_27259 http://aims.fao.org/aos/agrovoc/c_3225 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36375 H20 - Maladies des plantes Musa Mycosphaerella fijiensis génotype microsatellite marqueur génétique maladie des raies noires http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_27259 http://aims.fao.org/aos/agrovoc/c_3225 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36375 |
spellingShingle |
H20 - Maladies des plantes Musa Mycosphaerella fijiensis génotype microsatellite marqueur génétique maladie des raies noires http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_27259 http://aims.fao.org/aos/agrovoc/c_3225 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36375 H20 - Maladies des plantes Musa Mycosphaerella fijiensis génotype microsatellite marqueur génétique maladie des raies noires http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_27259 http://aims.fao.org/aos/agrovoc/c_3225 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36375 Robert, Stéphanie Rieux, Adrien Argout, Xavier Carlier, Jean Zapater, Marie-Françoise Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae) |
description |
Premise of the study: Large-scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fi jiensis , a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modifi ed DNA extraction protocol. Methods and Results: Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis. A total of 19 new polymorphic and easy-to-score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. Conclusions: The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis, allowing large-scale analysis of samples from various geographic locations. |
format |
article |
topic_facet |
H20 - Maladies des plantes Musa Mycosphaerella fijiensis génotype microsatellite marqueur génétique maladie des raies noires http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_27259 http://aims.fao.org/aos/agrovoc/c_3225 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36375 |
author |
Robert, Stéphanie Rieux, Adrien Argout, Xavier Carlier, Jean Zapater, Marie-Françoise |
author_facet |
Robert, Stéphanie Rieux, Adrien Argout, Xavier Carlier, Jean Zapater, Marie-Françoise |
author_sort |
Robert, Stéphanie |
title |
Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae) |
title_short |
Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae) |
title_full |
Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae) |
title_fullStr |
Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae) |
title_full_unstemmed |
Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae) |
title_sort |
optimized genotyping with microsatellite markers in the fungal banana pathogen mycosphaerella fijiensis (mycosphaerellaceae) |
url |
http://agritrop.cirad.fr/557717/ http://agritrop.cirad.fr/557717/1/document_557717.pdf |
work_keys_str_mv |
AT robertstephanie optimizedgenotypingwithmicrosatellitemarkersinthefungalbananapathogenmycosphaerellafijiensismycosphaerellaceae AT rieuxadrien optimizedgenotypingwithmicrosatellitemarkersinthefungalbananapathogenmycosphaerellafijiensismycosphaerellaceae AT argoutxavier optimizedgenotypingwithmicrosatellitemarkersinthefungalbananapathogenmycosphaerellafijiensismycosphaerellaceae AT carlierjean optimizedgenotypingwithmicrosatellitemarkersinthefungalbananapathogenmycosphaerellafijiensismycosphaerellaceae AT zapatermariefrancoise optimizedgenotypingwithmicrosatellitemarkersinthefungalbananapathogenmycosphaerellafijiensismycosphaerellaceae |
_version_ |
1792497698724642816 |