Identification of genes involved in the response of banana to crown rot disease
Variations in banana susceptibility to crown rot disease have been observed but the molecular mechanisms underlying these quantitative host-pathogen relationships are still unknown. This study was designed to compare gene expression between crowns of banana fruit showing a high susceptibility (S+) and crowns showing a low susceptibility (S-) to the disease. Comparisons were performed at two situation times: i) between crowns (S+ and S-) collected 1 h before inoculation and ii) between crowns (S+ and S-) collected 13 days after inoculation. Gene expression comparisons were performed with cDNA-amplified fragment length polymorphism (AFLP) and results were confirmed by realtime reverse-transcription polymerase chain reaction. Among genes identified as differentially expressed between S+ and S- crowns, two were involved in signal transduction, three in proteolytic machinery, two had similarity to pathogenesis- related protein 14, one to a CCR4-associated factor protein, and one to a cellulose synthase. Paradoxically, the overexpression of the cellulose synthase gene was associated with banana showing a high susceptibility in both preand post-inoculation situations. Finally, the cDNA-AFLP identified a gene that seems to be associated with the quantitative banana responses to crown rot disease; this gene encodes a dopamine-?-monooxygenase, which is involved in the catecholamine pathway. To our knowledge, this work is the first to address both pre- and post-infection gene expression with the same host-pathogen combination and distinct susceptibility levels.
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Subjects: | H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes, J11 - Manutention, transport, stockage et conservation des produits d'origine végétale, Musa, banane, pourriture, maladie postrécolte, résistance génétique, gène, http://aims.fao.org/aos/agrovoc/c_4993, http://aims.fao.org/aos/agrovoc/c_806, http://aims.fao.org/aos/agrovoc/c_6667, http://aims.fao.org/aos/agrovoc/c_36841, http://aims.fao.org/aos/agrovoc/c_35130, http://aims.fao.org/aos/agrovoc/c_3214, |
Online Access: | http://agritrop.cirad.fr/557624/ http://agritrop.cirad.fr/557624/1/document_557624.pdf |
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dig-cirad-fr-5576242024-01-28T18:46:22Z http://agritrop.cirad.fr/557624/ http://agritrop.cirad.fr/557624/ Identification of genes involved in the response of banana to crown rot disease. Lassois Ludivine, Frettinger Patrick, De Lapeyre de Bellaire Luc, Lepoivre Philippe, Jijakli M. Haïssam. 2011. Molecular Plant-Microbe Interactions, 24 (1) : 143-153.https://doi.org/10.1094/MPMI-01-10-0020 <https://doi.org/10.1094/MPMI-01-10-0020> Identification of genes involved in the response of banana to crown rot disease Lassois, Ludivine Frettinger, Patrick De Lapeyre de Bellaire, Luc Lepoivre, Philippe Jijakli, M. Haïssam eng 2011 Molecular Plant-Microbe Interactions H20 - Maladies des plantes F30 - Génétique et amélioration des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa banane pourriture maladie postrécolte résistance génétique gène http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_806 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_3214 Variations in banana susceptibility to crown rot disease have been observed but the molecular mechanisms underlying these quantitative host-pathogen relationships are still unknown. This study was designed to compare gene expression between crowns of banana fruit showing a high susceptibility (S+) and crowns showing a low susceptibility (S-) to the disease. Comparisons were performed at two situation times: i) between crowns (S+ and S-) collected 1 h before inoculation and ii) between crowns (S+ and S-) collected 13 days after inoculation. Gene expression comparisons were performed with cDNA-amplified fragment length polymorphism (AFLP) and results were confirmed by realtime reverse-transcription polymerase chain reaction. Among genes identified as differentially expressed between S+ and S- crowns, two were involved in signal transduction, three in proteolytic machinery, two had similarity to pathogenesis- related protein 14, one to a CCR4-associated factor protein, and one to a cellulose synthase. Paradoxically, the overexpression of the cellulose synthase gene was associated with banana showing a high susceptibility in both preand post-inoculation situations. Finally, the cDNA-AFLP identified a gene that seems to be associated with the quantitative banana responses to crown rot disease; this gene encodes a dopamine-?-monooxygenase, which is involved in the catecholamine pathway. To our knowledge, this work is the first to address both pre- and post-infection gene expression with the same host-pathogen combination and distinct susceptibility levels. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/557624/1/document_557624.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1094/MPMI-01-10-0020 10.1094/MPMI-01-10-0020 info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-01-10-0020 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1094/MPMI-01-10-0020 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa banane pourriture maladie postrécolte résistance génétique gène http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_806 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_3214 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa banane pourriture maladie postrécolte résistance génétique gène http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_806 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_3214 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa banane pourriture maladie postrécolte résistance génétique gène http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_806 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_3214 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa banane pourriture maladie postrécolte résistance génétique gène http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_806 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_3214 Lassois, Ludivine Frettinger, Patrick De Lapeyre de Bellaire, Luc Lepoivre, Philippe Jijakli, M. Haïssam Identification of genes involved in the response of banana to crown rot disease |
description |
Variations in banana susceptibility to crown rot disease have been observed but the molecular mechanisms underlying these quantitative host-pathogen relationships are still unknown. This study was designed to compare gene expression between crowns of banana fruit showing a high susceptibility (S+) and crowns showing a low susceptibility (S-) to the disease. Comparisons were performed at two situation times: i) between crowns (S+ and S-) collected 1 h before inoculation and ii) between crowns (S+ and S-) collected 13 days after inoculation. Gene expression comparisons were performed with cDNA-amplified fragment length polymorphism (AFLP) and results were confirmed by realtime reverse-transcription polymerase chain reaction. Among genes identified as differentially expressed between S+ and S- crowns, two were involved in signal transduction, three in proteolytic machinery, two had similarity to pathogenesis- related protein 14, one to a CCR4-associated factor protein, and one to a cellulose synthase. Paradoxically, the overexpression of the cellulose synthase gene was associated with banana showing a high susceptibility in both preand post-inoculation situations. Finally, the cDNA-AFLP identified a gene that seems to be associated with the quantitative banana responses to crown rot disease; this gene encodes a dopamine-?-monooxygenase, which is involved in the catecholamine pathway. To our knowledge, this work is the first to address both pre- and post-infection gene expression with the same host-pathogen combination and distinct susceptibility levels. |
format |
article |
topic_facet |
H20 - Maladies des plantes F30 - Génétique et amélioration des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale Musa banane pourriture maladie postrécolte résistance génétique gène http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_806 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_36841 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_3214 |
author |
Lassois, Ludivine Frettinger, Patrick De Lapeyre de Bellaire, Luc Lepoivre, Philippe Jijakli, M. Haïssam |
author_facet |
Lassois, Ludivine Frettinger, Patrick De Lapeyre de Bellaire, Luc Lepoivre, Philippe Jijakli, M. Haïssam |
author_sort |
Lassois, Ludivine |
title |
Identification of genes involved in the response of banana to crown rot disease |
title_short |
Identification of genes involved in the response of banana to crown rot disease |
title_full |
Identification of genes involved in the response of banana to crown rot disease |
title_fullStr |
Identification of genes involved in the response of banana to crown rot disease |
title_full_unstemmed |
Identification of genes involved in the response of banana to crown rot disease |
title_sort |
identification of genes involved in the response of banana to crown rot disease |
url |
http://agritrop.cirad.fr/557624/ http://agritrop.cirad.fr/557624/1/document_557624.pdf |
work_keys_str_mv |
AT lassoisludivine identificationofgenesinvolvedintheresponseofbananatocrownrotdisease AT frettingerpatrick identificationofgenesinvolvedintheresponseofbananatocrownrotdisease AT delapeyredebellaireluc identificationofgenesinvolvedintheresponseofbananatocrownrotdisease AT lepoivrephilippe identificationofgenesinvolvedintheresponseofbananatocrownrotdisease AT jijaklimhaissam identificationofgenesinvolvedintheresponseofbananatocrownrotdisease |
_version_ |
1792497692691136512 |