Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens

Genes of the Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC) coding for proteins capable of eliciting protective T-cell memory responses have potential for incorporation into a recombinant subunit vaccine against contagious bovine pleuropneumonia (CBPP). Here we used lymphocytes from cattle that had completely recovered from infection to screen products of MmmSC genes for recognition by CD4+ effector memory (Tem) and central memory (Tcm) T lymphocytes. Six MmmSC genes (abc, gapN, glpO, lppA, lppB, and ptsG) were expressed as histidine-tagged recombinant polypeptides, or synthetic overlapping peptides, before inclusion in proliferation and gamma interferon (IFN- ) assays. Only two MmmSC antigens, LppA and PtsG, consistently induced recall proliferation from immune CD4+ T cells and IFN- production in all animals tested. Moreover, LppA and PtsG were shown to possess epitopes recognized by both short-lived CD4+ Tem and long-lived CD4+ Tcm cells.

Bibliographic Details

Main Authors:	Totté, Philippe, Mather, Arshad, Reslan, Lina, Boublik, Yan, Niang, Mamadou, Du Plessis, Dion H., Dedieu, Laurence
Format:	article biblioteca
Language:	eng
Subjects:	L73 - Maladies des animaux, L10 - Génétique et amélioration des animaux, péripneumonie contagieuse bovine, Mycoplasma mycoides, identification, bovin, biotype, vaccin, antigène, protéine, immunologie, immunostimulant, gène, http://aims.fao.org/aos/agrovoc/c_16706, http://aims.fao.org/aos/agrovoc/c_23951, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_1391, http://aims.fao.org/aos/agrovoc/c_16166, http://aims.fao.org/aos/agrovoc/c_8130, http://aims.fao.org/aos/agrovoc/c_504, http://aims.fao.org/aos/agrovoc/c_6259, http://aims.fao.org/aos/agrovoc/c_3808, http://aims.fao.org/aos/agrovoc/c_27557, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_166,
Online Access:	http://agritrop.cirad.fr/557490/ http://agritrop.cirad.fr/557490/1/Clin.%20Vaccine%20Immunol.-2010-Tott%C3%A9-1211-6.pdf
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