Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens
Genes of the Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC) coding for proteins capable of eliciting protective T-cell memory responses have potential for incorporation into a recombinant subunit vaccine against contagious bovine pleuropneumonia (CBPP). Here we used lymphocytes from cattle that had completely recovered from infection to screen products of MmmSC genes for recognition by CD4+ effector memory (Tem) and central memory (Tcm) T lymphocytes. Six MmmSC genes (abc, gapN, glpO, lppA, lppB, and ptsG) were expressed as histidine-tagged recombinant polypeptides, or synthetic overlapping peptides, before inclusion in proliferation and gamma interferon (IFN- ) assays. Only two MmmSC antigens, LppA and PtsG, consistently induced recall proliferation from immune CD4+ T cells and IFN- production in all animals tested. Moreover, LppA and PtsG were shown to possess epitopes recognized by both short-lived CD4+ Tem and long-lived CD4+ Tcm cells.
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Subjects: | L73 - Maladies des animaux, L10 - Génétique et amélioration des animaux, péripneumonie contagieuse bovine, Mycoplasma mycoides, identification, bovin, biotype, vaccin, antigène, protéine, immunologie, immunostimulant, gène, http://aims.fao.org/aos/agrovoc/c_16706, http://aims.fao.org/aos/agrovoc/c_23951, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_1391, http://aims.fao.org/aos/agrovoc/c_16166, http://aims.fao.org/aos/agrovoc/c_8130, http://aims.fao.org/aos/agrovoc/c_504, http://aims.fao.org/aos/agrovoc/c_6259, http://aims.fao.org/aos/agrovoc/c_3808, http://aims.fao.org/aos/agrovoc/c_27557, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_166, |
Online Access: | http://agritrop.cirad.fr/557490/ http://agritrop.cirad.fr/557490/1/Clin.%20Vaccine%20Immunol.-2010-Tott%C3%A9-1211-6.pdf |
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dig-cirad-fr-5574902024-02-16T19:02:49Z http://agritrop.cirad.fr/557490/ http://agritrop.cirad.fr/557490/ Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens. Totté Philippe, Mather Arshad, Reslan Lina, Boublik Yan, Niang Mamadou, Du Plessis Dion H., Dedieu Laurence. 2010. Clinical and Vaccine Immunology, 17 (8) : 1211-1216.https://doi.org/10.1128/CVI.00132-10 <https://doi.org/10.1128/CVI.00132-10> Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens Totté, Philippe Mather, Arshad Reslan, Lina Boublik, Yan Niang, Mamadou Du Plessis, Dion H. Dedieu, Laurence eng 2010 Clinical and Vaccine Immunology L73 - Maladies des animaux L10 - Génétique et amélioration des animaux péripneumonie contagieuse bovine Mycoplasma mycoides identification bovin biotype vaccin antigène protéine immunologie immunostimulant gène http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_16166 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_504 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_27557 http://aims.fao.org/aos/agrovoc/c_3214 Afrique au sud du Sahara http://aims.fao.org/aos/agrovoc/c_166 Genes of the Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC) coding for proteins capable of eliciting protective T-cell memory responses have potential for incorporation into a recombinant subunit vaccine against contagious bovine pleuropneumonia (CBPP). Here we used lymphocytes from cattle that had completely recovered from infection to screen products of MmmSC genes for recognition by CD4+ effector memory (Tem) and central memory (Tcm) T lymphocytes. Six MmmSC genes (abc, gapN, glpO, lppA, lppB, and ptsG) were expressed as histidine-tagged recombinant polypeptides, or synthetic overlapping peptides, before inclusion in proliferation and gamma interferon (IFN- ) assays. Only two MmmSC antigens, LppA and PtsG, consistently induced recall proliferation from immune CD4+ T cells and IFN- production in all animals tested. Moreover, LppA and PtsG were shown to possess epitopes recognized by both short-lived CD4+ Tem and long-lived CD4+ Tcm cells. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/557490/1/Clin.%20Vaccine%20Immunol.-2010-Tott%C3%A9-1211-6.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1128/CVI.00132-10 10.1128/CVI.00132-10 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=209557 info:eu-repo/semantics/altIdentifier/doi/10.1128/CVI.00132-10 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1128/CVI.00132-10 |
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L73 - Maladies des animaux L10 - Génétique et amélioration des animaux péripneumonie contagieuse bovine Mycoplasma mycoides identification bovin biotype vaccin antigène protéine immunologie immunostimulant gène http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_16166 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_504 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_27557 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_166 L73 - Maladies des animaux L10 - Génétique et amélioration des animaux péripneumonie contagieuse bovine Mycoplasma mycoides identification bovin biotype vaccin antigène protéine immunologie immunostimulant gène http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_16166 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_504 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_27557 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_166 |
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L73 - Maladies des animaux L10 - Génétique et amélioration des animaux péripneumonie contagieuse bovine Mycoplasma mycoides identification bovin biotype vaccin antigène protéine immunologie immunostimulant gène http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_16166 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_504 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_27557 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_166 L73 - Maladies des animaux L10 - Génétique et amélioration des animaux péripneumonie contagieuse bovine Mycoplasma mycoides identification bovin biotype vaccin antigène protéine immunologie immunostimulant gène http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_16166 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_504 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_27557 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_166 Totté, Philippe Mather, Arshad Reslan, Lina Boublik, Yan Niang, Mamadou Du Plessis, Dion H. Dedieu, Laurence Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens |
description |
Genes of the Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC) coding for proteins capable of eliciting protective T-cell memory responses have potential for incorporation into a recombinant subunit vaccine against contagious bovine pleuropneumonia (CBPP). Here we used lymphocytes from cattle that had completely recovered from infection to screen products of MmmSC genes for recognition by CD4+ effector memory (Tem) and central memory (Tcm) T lymphocytes. Six MmmSC genes (abc, gapN, glpO, lppA, lppB, and ptsG) were expressed as histidine-tagged recombinant polypeptides, or synthetic overlapping peptides, before inclusion in proliferation and gamma interferon (IFN- ) assays. Only two MmmSC antigens, LppA and PtsG, consistently induced recall proliferation from immune CD4+ T cells and IFN- production in all animals tested. Moreover, LppA and PtsG were shown to possess epitopes recognized by both short-lived CD4+ Tem and long-lived CD4+ Tcm cells. |
format |
article |
topic_facet |
L73 - Maladies des animaux L10 - Génétique et amélioration des animaux péripneumonie contagieuse bovine Mycoplasma mycoides identification bovin biotype vaccin antigène protéine immunologie immunostimulant gène http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_16166 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_504 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_27557 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_166 |
author |
Totté, Philippe Mather, Arshad Reslan, Lina Boublik, Yan Niang, Mamadou Du Plessis, Dion H. Dedieu, Laurence |
author_facet |
Totté, Philippe Mather, Arshad Reslan, Lina Boublik, Yan Niang, Mamadou Du Plessis, Dion H. Dedieu, Laurence |
author_sort |
Totté, Philippe |
title |
Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens |
title_short |
Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens |
title_full |
Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens |
title_fullStr |
Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens |
title_full_unstemmed |
Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens |
title_sort |
identification of mycoplasma mycoides subsp. mycoides small colony genes coding for t-cell antigens |
url |
http://agritrop.cirad.fr/557490/ http://agritrop.cirad.fr/557490/1/Clin.%20Vaccine%20Immunol.-2010-Tott%C3%A9-1211-6.pdf |
work_keys_str_mv |
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