A new type of strain of Xanthomonas euvesicatoria causing bacterial spot of tomato and pepper in Grenada
Bacterial spot of tomato and pepper (BSTP) can be caused by several #Xanthomonas genospecies#. BSTP is a major disease in Grenada where A and B phenotypic groups (#Xanthomonas euvesicatoria# and #X. vesicatoria#, respectively, [2]) have been reported. There is no previous report of group A strains, which are strongly amylolytic and pectolytic, in Grenada. In March 2007, tomato and pepper leaves with lesions typical of BSTP were collected in Saint David and Saint Andrew parishes of Grenada. Bacterial isolations were performed on KC semiselective agar medium (4), resulting in isolation of five yellow-pigmented, #Xanthomonas#-like strains. Three strains isolated from tomato or pepper in Saint David were negative for starch hydrolysis and pectate degradation, two tests that were found useful for strain identification in the 1990s (2). Two strains isolated from pepper in Saint David were strongly amylolytic and degraded pectate. Amplified fragment length polymorphism (AFLP) and multilocus sequence analysis (MLSA) assays targeting atpD, dnaK, efp, and gyrB were performed on the five strains from Grenada together with a type strain of each of #X. euvesicatoria#, #X. perforans#, #X. gardneri#, and #X. vesicatoria# as well as other reference strains of #X. euvesicatoria# and #X. perforans# as described previously. All strains from Grenada were identified as #X. euvesicatoria# regardless of the typing technique. On the basis of AFLP assays, the two strains with phenotypic features not reported in Grenada were closely related (distances of ?0.002 nucleotide substitutions per site [1]) to a group of strains from India (ICMP 3381, LMG 907, LMG 908, and LMG 918). These two strains were also identical to the Indian strains based on MLSA, but differed from the #X. euvesicatoria# type strain by at least one nucleotide substitution in all loci examined. The three strains from Grenada that were negative for starch hydrolysis and pectate degradation had sequences identical to that of the type strain. Young leaves of tomato plants of cv. Marmande and pepper plants of cvs. Yolo Wonder and Aiguille were infiltrated (six inoculation sites per leaf, three replicate plants per cultivar per experiment, and the experiment was replicated once) using inoculum of each of the five strains from Grenada made from suspensions in Tris buffer containing approximately 1 × 105 CFU/ml. Two reference strains of #X. euvesicatoria# (NCPPB 2968 and LMG 922) were also inoculated as positive control treatments. Negative control treatments consisted of leaves infiltrated with sterile Tris buffer. Typical water-soaked lesions that developed into necrotic spots were observed 3 to 8 days after inoculation (dai) for all strains on all cultivars, except NCPPB 2968, which was not pathogenic on pepper cv. Aiguille. #Xanthomonas# population sizes from lesions plated onto KC agar medium (4) 25 dai ranged from 3 × 106 to 5 × 107, 8 × 107 to 2 × 108, and 9 × 106 to 2 × 108 CFU/lesion on tomato cv. Marmande and pepper cvs. Yolo Wonder and Aiguille, respectively. The epidemiological importance of this previously unreported group of #X. euvesicatoria# strains in Grenada needs to be assessed.
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Subjects: | H20 - Maladies des plantes, Xanthomonas, Solanum lycopersicum, Capsicum annuum, http://aims.fao.org/aos/agrovoc/c_8455, http://aims.fao.org/aos/agrovoc/c_4475, http://aims.fao.org/aos/agrovoc/c_1288, http://aims.fao.org/aos/agrovoc/c_6543, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/557231/ http://agritrop.cirad.fr/557231/1/document_557231.pdf |
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H20 - Maladies des plantes Xanthomonas Solanum lycopersicum Capsicum annuum http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_4475 http://aims.fao.org/aos/agrovoc/c_1288 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Xanthomonas Solanum lycopersicum Capsicum annuum http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_4475 http://aims.fao.org/aos/agrovoc/c_1288 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
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H20 - Maladies des plantes Xanthomonas Solanum lycopersicum Capsicum annuum http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_4475 http://aims.fao.org/aos/agrovoc/c_1288 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Xanthomonas Solanum lycopersicum Capsicum annuum http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_4475 http://aims.fao.org/aos/agrovoc/c_1288 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Hamza, Abdou Azali Robène-Soustrade, Isabelle Boyer, Claudine Laurent, Annie Jouen, Emmanuel Wicker, Emmanuel Prior, Philippe Pruvost, Olivier A new type of strain of Xanthomonas euvesicatoria causing bacterial spot of tomato and pepper in Grenada |
description |
Bacterial spot of tomato and pepper (BSTP) can be caused by several #Xanthomonas genospecies#. BSTP is a major disease in Grenada where A and B phenotypic groups (#Xanthomonas euvesicatoria# and #X. vesicatoria#, respectively, [2]) have been reported. There is no previous report of group A strains, which are strongly amylolytic and pectolytic, in Grenada. In March 2007, tomato and pepper leaves with lesions typical of BSTP were collected in Saint David and Saint Andrew parishes of Grenada. Bacterial isolations were performed on KC semiselective agar medium (4), resulting in isolation of five yellow-pigmented, #Xanthomonas#-like strains. Three strains isolated from tomato or pepper in Saint David were negative for starch hydrolysis and pectate degradation, two tests that were found useful for strain identification in the 1990s (2). Two strains isolated from pepper in Saint David were strongly amylolytic and degraded pectate. Amplified fragment length polymorphism (AFLP) and multilocus sequence analysis (MLSA) assays targeting atpD, dnaK, efp, and gyrB were performed on the five strains from Grenada together with a type strain of each of #X. euvesicatoria#, #X. perforans#, #X. gardneri#, and #X. vesicatoria# as well as other reference strains of #X. euvesicatoria# and #X. perforans# as described previously. All strains from Grenada were identified as #X. euvesicatoria# regardless of the typing technique. On the basis of AFLP assays, the two strains with phenotypic features not reported in Grenada were closely related (distances of ?0.002 nucleotide substitutions per site [1]) to a group of strains from India (ICMP 3381, LMG 907, LMG 908, and LMG 918). These two strains were also identical to the Indian strains based on MLSA, but differed from the #X. euvesicatoria# type strain by at least one nucleotide substitution in all loci examined. The three strains from Grenada that were negative for starch hydrolysis and pectate degradation had sequences identical to that of the type strain. Young leaves of tomato plants of cv. Marmande and pepper plants of cvs. Yolo Wonder and Aiguille were infiltrated (six inoculation sites per leaf, three replicate plants per cultivar per experiment, and the experiment was replicated once) using inoculum of each of the five strains from Grenada made from suspensions in Tris buffer containing approximately 1 × 105 CFU/ml. Two reference strains of #X. euvesicatoria# (NCPPB 2968 and LMG 922) were also inoculated as positive control treatments. Negative control treatments consisted of leaves infiltrated with sterile Tris buffer. Typical water-soaked lesions that developed into necrotic spots were observed 3 to 8 days after inoculation (dai) for all strains on all cultivars, except NCPPB 2968, which was not pathogenic on pepper cv. Aiguille. #Xanthomonas# population sizes from lesions plated onto KC agar medium (4) 25 dai ranged from 3 × 106 to 5 × 107, 8 × 107 to 2 × 108, and 9 × 106 to 2 × 108 CFU/lesion on tomato cv. Marmande and pepper cvs. Yolo Wonder and Aiguille, respectively. The epidemiological importance of this previously unreported group of #X. euvesicatoria# strains in Grenada needs to be assessed. |
format |
article |
topic_facet |
H20 - Maladies des plantes Xanthomonas Solanum lycopersicum Capsicum annuum http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_4475 http://aims.fao.org/aos/agrovoc/c_1288 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Hamza, Abdou Azali Robène-Soustrade, Isabelle Boyer, Claudine Laurent, Annie Jouen, Emmanuel Wicker, Emmanuel Prior, Philippe Pruvost, Olivier |
author_facet |
Hamza, Abdou Azali Robène-Soustrade, Isabelle Boyer, Claudine Laurent, Annie Jouen, Emmanuel Wicker, Emmanuel Prior, Philippe Pruvost, Olivier |
author_sort |
Hamza, Abdou Azali |
title |
A new type of strain of Xanthomonas euvesicatoria causing bacterial spot of tomato and pepper in Grenada |
title_short |
A new type of strain of Xanthomonas euvesicatoria causing bacterial spot of tomato and pepper in Grenada |
title_full |
A new type of strain of Xanthomonas euvesicatoria causing bacterial spot of tomato and pepper in Grenada |
title_fullStr |
A new type of strain of Xanthomonas euvesicatoria causing bacterial spot of tomato and pepper in Grenada |
title_full_unstemmed |
A new type of strain of Xanthomonas euvesicatoria causing bacterial spot of tomato and pepper in Grenada |
title_sort |
new type of strain of xanthomonas euvesicatoria causing bacterial spot of tomato and pepper in grenada |
url |
http://agritrop.cirad.fr/557231/ http://agritrop.cirad.fr/557231/1/document_557231.pdf |
work_keys_str_mv |
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dig-cirad-fr-5572312024-01-28T18:42:12Z http://agritrop.cirad.fr/557231/ http://agritrop.cirad.fr/557231/ A new type of strain of Xanthomonas euvesicatoria causing bacterial spot of tomato and pepper in Grenada. Hamza Abdou Azali, Robène-Soustrade Isabelle, Boyer Claudine, Laurent Annie, Jouen Emmanuel, Wicker Emmanuel, Prior Philippe, Pruvost Olivier. 2010. Plant Disease, 94 (10) : 1264.https://doi.org/10.1094/PDIS-05-10-0397 <https://doi.org/10.1094/PDIS-05-10-0397> A new type of strain of Xanthomonas euvesicatoria causing bacterial spot of tomato and pepper in Grenada Hamza, Abdou Azali Robène-Soustrade, Isabelle Boyer, Claudine Laurent, Annie Jouen, Emmanuel Wicker, Emmanuel Prior, Philippe Pruvost, Olivier eng 2010 Plant Disease H20 - Maladies des plantes Xanthomonas Solanum lycopersicum Capsicum annuum http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_4475 http://aims.fao.org/aos/agrovoc/c_1288 La Réunion France http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Bacterial spot of tomato and pepper (BSTP) can be caused by several #Xanthomonas genospecies#. BSTP is a major disease in Grenada where A and B phenotypic groups (#Xanthomonas euvesicatoria# and #X. vesicatoria#, respectively, [2]) have been reported. There is no previous report of group A strains, which are strongly amylolytic and pectolytic, in Grenada. In March 2007, tomato and pepper leaves with lesions typical of BSTP were collected in Saint David and Saint Andrew parishes of Grenada. Bacterial isolations were performed on KC semiselective agar medium (4), resulting in isolation of five yellow-pigmented, #Xanthomonas#-like strains. Three strains isolated from tomato or pepper in Saint David were negative for starch hydrolysis and pectate degradation, two tests that were found useful for strain identification in the 1990s (2). Two strains isolated from pepper in Saint David were strongly amylolytic and degraded pectate. Amplified fragment length polymorphism (AFLP) and multilocus sequence analysis (MLSA) assays targeting atpD, dnaK, efp, and gyrB were performed on the five strains from Grenada together with a type strain of each of #X. euvesicatoria#, #X. perforans#, #X. gardneri#, and #X. vesicatoria# as well as other reference strains of #X. euvesicatoria# and #X. perforans# as described previously. All strains from Grenada were identified as #X. euvesicatoria# regardless of the typing technique. On the basis of AFLP assays, the two strains with phenotypic features not reported in Grenada were closely related (distances of ?0.002 nucleotide substitutions per site [1]) to a group of strains from India (ICMP 3381, LMG 907, LMG 908, and LMG 918). These two strains were also identical to the Indian strains based on MLSA, but differed from the #X. euvesicatoria# type strain by at least one nucleotide substitution in all loci examined. The three strains from Grenada that were negative for starch hydrolysis and pectate degradation had sequences identical to that of the type strain. Young leaves of tomato plants of cv. Marmande and pepper plants of cvs. Yolo Wonder and Aiguille were infiltrated (six inoculation sites per leaf, three replicate plants per cultivar per experiment, and the experiment was replicated once) using inoculum of each of the five strains from Grenada made from suspensions in Tris buffer containing approximately 1 × 105 CFU/ml. Two reference strains of #X. euvesicatoria# (NCPPB 2968 and LMG 922) were also inoculated as positive control treatments. Negative control treatments consisted of leaves infiltrated with sterile Tris buffer. Typical water-soaked lesions that developed into necrotic spots were observed 3 to 8 days after inoculation (dai) for all strains on all cultivars, except NCPPB 2968, which was not pathogenic on pepper cv. Aiguille. #Xanthomonas# population sizes from lesions plated onto KC agar medium (4) 25 dai ranged from 3 × 106 to 5 × 107, 8 × 107 to 2 × 108, and 9 × 106 to 2 × 108 CFU/lesion on tomato cv. Marmande and pepper cvs. Yolo Wonder and Aiguille, respectively. The epidemiological importance of this previously unreported group of #X. euvesicatoria# strains in Grenada needs to be assessed. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/557231/1/document_557231.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1094/PDIS-05-10-0397 10.1094/PDIS-05-10-0397 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=209220 info:eu-repo/semantics/altIdentifier/doi/10.1094/PDIS-05-10-0397 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1094/PDIS-05-10-0397 |