Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype
Isolates of ectomycorrhizal Pisolithus albus were sampled from both ultramafic and volcano-sedimentary soils in New Caledonia, a tropical hotspot of biodiversity, to investigate the relationships between genetic diversity and edaphic constraint through tolerance to nickel (Ni). Carpophore description, spore morphology and phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequences confirmed that all isolates belong to P. albus and are closely related to other Australasian specimens. Using molecular tools, ITS-restriction fragment length polymorphism and amplified fragment length polymorphism markers, we showed the existence of two distinct genetic clusters within P. albus: ultramafic and volcano-sedimentary. Mycelia response to Ni toxicity supports such a population structure. Pisolithus albus from ultramafic soils included isolates with a high diversity of in vitro Ni tolerance, with both Ni-tolerant isolates (average Ni EC50 at 575 mM) and Ni-sensitive isolates (average Ni EC50 at 37 mM). In contrast, all isolates from volcano-sedimentary soils were found to be Ni sensitive (average Ni EC50 at 32 mM). We highlight that (1) P. albus population from ultramafic soils of New Caledonia are genetically structured in ecotype, and that (2) Ni tolerance among ultramafic isolates suggests an adaptive physiological response to Ni toxicity.
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Subjects: | P34 - Biologie du sol, P35 - Fertilité du sol, Pisolithus, Mycorhizé, symbiose, tolérance, fertilité du sol, nickel, type de sol chimique, métal lourd, http://aims.fao.org/aos/agrovoc/c_23961, http://aims.fao.org/aos/agrovoc/c_5023, http://aims.fao.org/aos/agrovoc/c_7563, http://aims.fao.org/aos/agrovoc/c_37740, http://aims.fao.org/aos/agrovoc/c_7170, http://aims.fao.org/aos/agrovoc/c_5172, http://aims.fao.org/aos/agrovoc/c_1519, http://aims.fao.org/aos/agrovoc/c_2223, http://aims.fao.org/aos/agrovoc/c_5155, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/556470/ http://agritrop.cirad.fr/556470/1/document_556470.pdf |
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dig-cirad-fr-5564702024-01-28T18:35:33Z http://agritrop.cirad.fr/556470/ http://agritrop.cirad.fr/556470/ Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype. Jourand Philippe, Ducousso Marc, Loulergue-Majorel Clarisse, Hannibal Laure, Santoni Sylvain, Prin Yves, Lebrun Michel. 2010. FEMS Microbiology, Ecology, 72 (2) : 238-249.https://doi.org/10.1111/j.1574-6941.2010.00843.x <https://doi.org/10.1111/j.1574-6941.2010.00843.x> Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype Jourand, Philippe Ducousso, Marc Loulergue-Majorel, Clarisse Hannibal, Laure Santoni, Sylvain Prin, Yves Lebrun, Michel eng 2010 FEMS Microbiology, Ecology P34 - Biologie du sol P35 - Fertilité du sol Pisolithus Mycorhizé symbiose tolérance fertilité du sol nickel type de sol chimique métal lourd http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_7170 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 Nouvelle-Calédonie France http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 Isolates of ectomycorrhizal Pisolithus albus were sampled from both ultramafic and volcano-sedimentary soils in New Caledonia, a tropical hotspot of biodiversity, to investigate the relationships between genetic diversity and edaphic constraint through tolerance to nickel (Ni). Carpophore description, spore morphology and phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequences confirmed that all isolates belong to P. albus and are closely related to other Australasian specimens. Using molecular tools, ITS-restriction fragment length polymorphism and amplified fragment length polymorphism markers, we showed the existence of two distinct genetic clusters within P. albus: ultramafic and volcano-sedimentary. Mycelia response to Ni toxicity supports such a population structure. Pisolithus albus from ultramafic soils included isolates with a high diversity of in vitro Ni tolerance, with both Ni-tolerant isolates (average Ni EC50 at 575 mM) and Ni-sensitive isolates (average Ni EC50 at 37 mM). In contrast, all isolates from volcano-sedimentary soils were found to be Ni sensitive (average Ni EC50 at 32 mM). We highlight that (1) P. albus population from ultramafic soils of New Caledonia are genetically structured in ecotype, and that (2) Ni tolerance among ultramafic isolates suggests an adaptive physiological response to Ni toxicity. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/556470/1/document_556470.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/j.1574-6941.2010.00843.x 10.1111/j.1574-6941.2010.00843.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6941.2010.00843.x info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/j.1574-6941.2010.00843.x |
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P34 - Biologie du sol P35 - Fertilité du sol Pisolithus Mycorhizé symbiose tolérance fertilité du sol nickel type de sol chimique métal lourd http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_7170 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 P34 - Biologie du sol P35 - Fertilité du sol Pisolithus Mycorhizé symbiose tolérance fertilité du sol nickel type de sol chimique métal lourd http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_7170 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 |
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P34 - Biologie du sol P35 - Fertilité du sol Pisolithus Mycorhizé symbiose tolérance fertilité du sol nickel type de sol chimique métal lourd http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_7170 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 P34 - Biologie du sol P35 - Fertilité du sol Pisolithus Mycorhizé symbiose tolérance fertilité du sol nickel type de sol chimique métal lourd http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_7170 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 Jourand, Philippe Ducousso, Marc Loulergue-Majorel, Clarisse Hannibal, Laure Santoni, Sylvain Prin, Yves Lebrun, Michel Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype |
description |
Isolates of ectomycorrhizal Pisolithus albus were sampled from both ultramafic and volcano-sedimentary soils in New Caledonia, a tropical hotspot of biodiversity, to investigate the relationships between genetic diversity and edaphic constraint through tolerance to nickel (Ni). Carpophore description, spore morphology and phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequences confirmed that all isolates belong to P. albus and are closely related to other Australasian specimens. Using molecular tools, ITS-restriction fragment length polymorphism and amplified fragment length polymorphism markers, we showed the existence of two distinct genetic clusters within P. albus: ultramafic and volcano-sedimentary. Mycelia response to Ni toxicity supports such a population structure. Pisolithus albus from ultramafic soils included isolates with a high diversity of in vitro Ni tolerance, with both Ni-tolerant isolates (average Ni EC50 at 575 mM) and Ni-sensitive isolates (average Ni EC50 at 37 mM). In contrast, all isolates from volcano-sedimentary soils were found to be Ni sensitive (average Ni EC50 at 32 mM). We highlight that (1) P. albus population from ultramafic soils of New Caledonia are genetically structured in ecotype, and that (2) Ni tolerance among ultramafic isolates suggests an adaptive physiological response to Ni toxicity. |
format |
article |
topic_facet |
P34 - Biologie du sol P35 - Fertilité du sol Pisolithus Mycorhizé symbiose tolérance fertilité du sol nickel type de sol chimique métal lourd http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_7170 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Jourand, Philippe Ducousso, Marc Loulergue-Majorel, Clarisse Hannibal, Laure Santoni, Sylvain Prin, Yves Lebrun, Michel |
author_facet |
Jourand, Philippe Ducousso, Marc Loulergue-Majorel, Clarisse Hannibal, Laure Santoni, Sylvain Prin, Yves Lebrun, Michel |
author_sort |
Jourand, Philippe |
title |
Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype |
title_short |
Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype |
title_full |
Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype |
title_fullStr |
Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype |
title_full_unstemmed |
Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype |
title_sort |
ultramafic soils from new caledonia structure pisolithus albus in ecotype |
url |
http://agritrop.cirad.fr/556470/ http://agritrop.cirad.fr/556470/1/document_556470.pdf |
work_keys_str_mv |
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