Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution
Quantitative information on growing organs is required to better understand morphogenesis in both plants and animals. However, detailed analyses of growth patterns at cellular resolution have remained elusive. We developed an approach, multiangle image acquisition, three-dimensional reconstruction and cell segmentation-automated lineage tracking (MARS-ALT), in which we imaged whole organs from multiple angles, computationally merged and segmented these images to provide accurate cell identification in three dimensions and automatically tracked cell lineages through multiple rounds of cell division during development. Using these methods, we quantitatively analyzed Arabidopsis thaliana flower development at cell resolution, which revealed differential growth patterns of key regions during early stages of floral morphogenesis. Lastly, using rice roots, we demonstrated that this approach is both generic and scalable.
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Subjects: | U10 - Informatique, mathématiques et statistiques, F50 - Anatomie et morphologie des plantes, F62 - Physiologie végétale - Croissance et développement, |
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dig-cirad-fr-5560322022-12-30T18:34:01Z http://agritrop.cirad.fr/556032/ http://agritrop.cirad.fr/556032/ Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution. Fernandez Romain, Das Pradeep, Mirabet Vincent, Moscardi Eric, Traas Jan, Verdeil Jean-Luc, Malandain Grégoire, Godin Christophe. 2010. Nature Methods, 7 (7) : 547-553.https://doi.org/10.1038/nmeth.1472 <https://doi.org/10.1038/nmeth.1472> Researchers Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution Fernandez, Romain Das, Pradeep Mirabet, Vincent Moscardi, Eric Traas, Jan Verdeil, Jean-Luc Malandain, Grégoire Godin, Christophe eng 2010 Nature Methods U10 - Informatique, mathématiques et statistiques F50 - Anatomie et morphologie des plantes F62 - Physiologie végétale - Croissance et développement Quantitative information on growing organs is required to better understand morphogenesis in both plants and animals. However, detailed analyses of growth patterns at cellular resolution have remained elusive. We developed an approach, multiangle image acquisition, three-dimensional reconstruction and cell segmentation-automated lineage tracking (MARS-ALT), in which we imaged whole organs from multiple angles, computationally merged and segmented these images to provide accurate cell identification in three dimensions and automatically tracked cell lineages through multiple rounds of cell division during development. Using these methods, we quantitatively analyzed Arabidopsis thaliana flower development at cell resolution, which revealed differential growth patterns of key regions during early stages of floral morphogenesis. Lastly, using rice roots, we demonstrated that this approach is both generic and scalable. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/556032/1/document_556032.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1038/nmeth.1472 10.1038/nmeth.1472 info:eu-repo/semantics/altIdentifier/doi/10.1038/nmeth.1472 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1038/nmeth.1472 |
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U10 - Informatique, mathématiques et statistiques F50 - Anatomie et morphologie des plantes F62 - Physiologie végétale - Croissance et développement U10 - Informatique, mathématiques et statistiques F50 - Anatomie et morphologie des plantes F62 - Physiologie végétale - Croissance et développement |
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U10 - Informatique, mathématiques et statistiques F50 - Anatomie et morphologie des plantes F62 - Physiologie végétale - Croissance et développement U10 - Informatique, mathématiques et statistiques F50 - Anatomie et morphologie des plantes F62 - Physiologie végétale - Croissance et développement Fernandez, Romain Das, Pradeep Mirabet, Vincent Moscardi, Eric Traas, Jan Verdeil, Jean-Luc Malandain, Grégoire Godin, Christophe Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution |
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Quantitative information on growing organs is required to better understand morphogenesis in both plants and animals. However, detailed analyses of growth patterns at cellular resolution have remained elusive. We developed an approach, multiangle image acquisition, three-dimensional reconstruction and cell segmentation-automated lineage tracking (MARS-ALT), in which we imaged whole organs from multiple angles, computationally merged and segmented these images to provide accurate cell identification in three dimensions and automatically tracked cell lineages through multiple rounds of cell division during development. Using these methods, we quantitatively analyzed Arabidopsis thaliana flower development at cell resolution, which revealed differential growth patterns of key regions during early stages of floral morphogenesis. Lastly, using rice roots, we demonstrated that this approach is both generic and scalable. |
format |
article |
topic_facet |
U10 - Informatique, mathématiques et statistiques F50 - Anatomie et morphologie des plantes F62 - Physiologie végétale - Croissance et développement |
author |
Fernandez, Romain Das, Pradeep Mirabet, Vincent Moscardi, Eric Traas, Jan Verdeil, Jean-Luc Malandain, Grégoire Godin, Christophe |
author_facet |
Fernandez, Romain Das, Pradeep Mirabet, Vincent Moscardi, Eric Traas, Jan Verdeil, Jean-Luc Malandain, Grégoire Godin, Christophe |
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Fernandez, Romain |
title |
Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution |
title_short |
Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution |
title_full |
Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution |
title_fullStr |
Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution |
title_full_unstemmed |
Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution |
title_sort |
imaging plant growth in 4d: robust tissue reconstruction and lineaging at cell resolution |
url |
http://agritrop.cirad.fr/556032/ http://agritrop.cirad.fr/556032/1/document_556032.pdf |
work_keys_str_mv |
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