Engineered transfer of the PKS/NRPS biosynthesis pathway of albicidin: A promising approach to overproduce this potent antibiotic
Xanthomonas albilineans, which causes leaf scald disease of sugarcane, produces a highly potent pathotoxin and antibiotic called albicidin that was shown to inhibit DNA replication in both sugarcane proplastids and Escherichia coli. Low yields of albicidin production in slow growing X. albilineans have slowed studies of its chemical structure and potential therapeutic applications. Albicidin is synthesized by a unique hybrid PKS/NRPS (polyketide synthase/nonribosomal peptide synthase) pathway that does not resemble any other described to date. We report here the transfer of the entire 49 kb albicidin biosynthetic gene cluster from X. albilineans into X. axonopodis pv. vesicatoria and the subsequent production of an antibiotic active against E. coli that shows cross-resistance with albicidin. The yield of this antibiotic in X. axonopodis pv. vesicatoria is 6 times higher than in X. albilineans. This study demonstrates the feasibility to transfer the albicidin pathway into an heterologous host and offers a promising strategy to overproduce, characterize and explore potential therapeutic applications of this potent antibiotic. (Texte intégral)
Main Authors: | , , , , , , , |
---|---|
Format: | conference_item biblioteca |
Language: | eng |
Published: |
s.n.
|
Subjects: | H20 - Maladies des plantes, |
Online Access: | http://agritrop.cirad.fr/553865/ http://agritrop.cirad.fr/553865/1/document_553865.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-553865 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5538652024-07-23T16:01:36Z http://agritrop.cirad.fr/553865/ http://agritrop.cirad.fr/553865/ Engineered transfer of the PKS/NRPS biosynthesis pathway of albicidin: A promising approach to overproduce this potent antibiotic. Cociancich Stéphane, Vivien Eric, Pitorre Delphine, Duplan Sandrine, Pieretti Isabelle, Gabriel Dean W., Rott Philippe, Royer Monique. 2006. In : Proceedings 2nd FEMS Congress of European Microbiologists, Madrid, Spain, 04-08 julio, 2006. s.l. : s.n., Résumé, 1 p. FEMS Congress of European Microbiologists. 2, Madrid, Espagne, 4 Juillet 2006/8 Juillet 2006. Engineered transfer of the PKS/NRPS biosynthesis pathway of albicidin: A promising approach to overproduce this potent antibiotic Cociancich, Stéphane Vivien, Eric Pitorre, Delphine Duplan, Sandrine Pieretti, Isabelle Gabriel, Dean W. Rott, Philippe Royer, Monique eng 2006 s.n. Proceedings 2nd FEMS Congress of European Microbiologists, Madrid, Spain, 04-08 julio, 2006 H20 - Maladies des plantes Xanthomonas albilineans, which causes leaf scald disease of sugarcane, produces a highly potent pathotoxin and antibiotic called albicidin that was shown to inhibit DNA replication in both sugarcane proplastids and Escherichia coli. Low yields of albicidin production in slow growing X. albilineans have slowed studies of its chemical structure and potential therapeutic applications. Albicidin is synthesized by a unique hybrid PKS/NRPS (polyketide synthase/nonribosomal peptide synthase) pathway that does not resemble any other described to date. We report here the transfer of the entire 49 kb albicidin biosynthetic gene cluster from X. albilineans into X. axonopodis pv. vesicatoria and the subsequent production of an antibiotic active against E. coli that shows cross-resistance with albicidin. The yield of this antibiotic in X. axonopodis pv. vesicatoria is 6 times higher than in X. albilineans. This study demonstrates the feasibility to transfer the albicidin pathway into an heterologous host and offers a promising strategy to overproduce, characterize and explore potential therapeutic applications of this potent antibiotic. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/553865/1/document_553865.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=207278 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H20 - Maladies des plantes H20 - Maladies des plantes |
spellingShingle |
H20 - Maladies des plantes H20 - Maladies des plantes Cociancich, Stéphane Vivien, Eric Pitorre, Delphine Duplan, Sandrine Pieretti, Isabelle Gabriel, Dean W. Rott, Philippe Royer, Monique Engineered transfer of the PKS/NRPS biosynthesis pathway of albicidin: A promising approach to overproduce this potent antibiotic |
description |
Xanthomonas albilineans, which causes leaf scald disease of sugarcane, produces a highly potent pathotoxin and antibiotic called albicidin that was shown to inhibit DNA replication in both sugarcane proplastids and Escherichia coli. Low yields of albicidin production in slow growing X. albilineans have slowed studies of its chemical structure and potential therapeutic applications. Albicidin is synthesized by a unique hybrid PKS/NRPS (polyketide synthase/nonribosomal peptide synthase) pathway that does not resemble any other described to date. We report here the transfer of the entire 49 kb albicidin biosynthetic gene cluster from X. albilineans into X. axonopodis pv. vesicatoria and the subsequent production of an antibiotic active against E. coli that shows cross-resistance with albicidin. The yield of this antibiotic in X. axonopodis pv. vesicatoria is 6 times higher than in X. albilineans. This study demonstrates the feasibility to transfer the albicidin pathway into an heterologous host and offers a promising strategy to overproduce, characterize and explore potential therapeutic applications of this potent antibiotic. (Texte intégral) |
format |
conference_item |
topic_facet |
H20 - Maladies des plantes |
author |
Cociancich, Stéphane Vivien, Eric Pitorre, Delphine Duplan, Sandrine Pieretti, Isabelle Gabriel, Dean W. Rott, Philippe Royer, Monique |
author_facet |
Cociancich, Stéphane Vivien, Eric Pitorre, Delphine Duplan, Sandrine Pieretti, Isabelle Gabriel, Dean W. Rott, Philippe Royer, Monique |
author_sort |
Cociancich, Stéphane |
title |
Engineered transfer of the PKS/NRPS biosynthesis pathway of albicidin: A promising approach to overproduce this potent antibiotic |
title_short |
Engineered transfer of the PKS/NRPS biosynthesis pathway of albicidin: A promising approach to overproduce this potent antibiotic |
title_full |
Engineered transfer of the PKS/NRPS biosynthesis pathway of albicidin: A promising approach to overproduce this potent antibiotic |
title_fullStr |
Engineered transfer of the PKS/NRPS biosynthesis pathway of albicidin: A promising approach to overproduce this potent antibiotic |
title_full_unstemmed |
Engineered transfer of the PKS/NRPS biosynthesis pathway of albicidin: A promising approach to overproduce this potent antibiotic |
title_sort |
engineered transfer of the pks/nrps biosynthesis pathway of albicidin: a promising approach to overproduce this potent antibiotic |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/553865/ http://agritrop.cirad.fr/553865/1/document_553865.pdf |
work_keys_str_mv |
AT cociancichstephane engineeredtransferofthepksnrpsbiosynthesispathwayofalbicidinapromisingapproachtooverproducethispotentantibiotic AT vivieneric engineeredtransferofthepksnrpsbiosynthesispathwayofalbicidinapromisingapproachtooverproducethispotentantibiotic AT pitorredelphine engineeredtransferofthepksnrpsbiosynthesispathwayofalbicidinapromisingapproachtooverproducethispotentantibiotic AT duplansandrine engineeredtransferofthepksnrpsbiosynthesispathwayofalbicidinapromisingapproachtooverproducethispotentantibiotic AT pierettiisabelle engineeredtransferofthepksnrpsbiosynthesispathwayofalbicidinapromisingapproachtooverproducethispotentantibiotic AT gabrieldeanw engineeredtransferofthepksnrpsbiosynthesispathwayofalbicidinapromisingapproachtooverproducethispotentantibiotic AT rottphilippe engineeredtransferofthepksnrpsbiosynthesispathwayofalbicidinapromisingapproachtooverproducethispotentantibiotic AT royermonique engineeredtransferofthepksnrpsbiosynthesispathwayofalbicidinapromisingapproachtooverproducethispotentantibiotic |
_version_ |
1806036927094194176 |