Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence
Background. In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus species are characterized by high heterozygosity, making it difficult to assemble large genome sequences. Thus, the International Citrus Genomic Consortium (ICGC) decided to establish a reference whole citrus genome sequence from a homozygous plant. Due to the existence of important molecular resources and previous success in obtaining haploid clementine plants, haploid clementine was selected as the target for the implementation of the reference whole genome citrus sequence. Results. To obtain haploid clementine lines we used the technique of in situ gynogenesis induced by irradiated pollen. Flow cytometry, chromosome counts and SSR marker (Simple Sequence Repeats) analysis facilitated the identification of six different haploid lines (2n = x = 9), one aneuploid line (2n = 2x+4 = 22) and one doubled haploid plant (2n = 2x = 18) of 'Clemenules' clementine. One of the haploids, obtained directly from an original haploid embryo, grew vigorously and produced flowers after four years. This is the first haploid plant of clementine that has bloomed and we have, for the first time, characterized the histology of haploid and diploid flowers of clementine. Additionally a double haploid plant was obtained spontaneously from this haploid line. Conclusion. The first haploid plant of 'Clemenules' clementine produced directly by germination of a haploid embryo, which grew vigorously and produced flowers, has been obtained in this work. This haploid line has been selected and it is being used by the ICGC to establish the reference sequence of the nuclear genome of citrus.
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Subjects: | F30 - Génétique et amélioration des plantes, Citrus, haploïdie, Citrus clementina, http://aims.fao.org/aos/agrovoc/c_1637, http://aims.fao.org/aos/agrovoc/c_3490, http://aims.fao.org/aos/agrovoc/c_6ce991dd, http://aims.fao.org/aos/agrovoc/c_7273, |
Online Access: | http://agritrop.cirad.fr/552434/ http://agritrop.cirad.fr/552434/1/document_552434.pdf |
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dig-cirad-fr-5524342024-01-28T17:50:09Z http://agritrop.cirad.fr/552434/ http://agritrop.cirad.fr/552434/ Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence. Aleza Pablo, Juarez José, Hernandez Maria, Pina José Antonio, Ollitrault Patrick, Navarro Luis. 2009. BMC Plant Biology, 9 (110), 17 p.https://doi.org/10.1186/1471-2229-9-110 <https://doi.org/10.1186/1471-2229-9-110> Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence Aleza, Pablo Juarez, José Hernandez, Maria Pina, José Antonio Ollitrault, Patrick Navarro, Luis eng 2009 BMC Plant Biology F30 - Génétique et amélioration des plantes Citrus haploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_3490 http://aims.fao.org/aos/agrovoc/c_6ce991dd Espagne http://aims.fao.org/aos/agrovoc/c_7273 Background. In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus species are characterized by high heterozygosity, making it difficult to assemble large genome sequences. Thus, the International Citrus Genomic Consortium (ICGC) decided to establish a reference whole citrus genome sequence from a homozygous plant. Due to the existence of important molecular resources and previous success in obtaining haploid clementine plants, haploid clementine was selected as the target for the implementation of the reference whole genome citrus sequence. Results. To obtain haploid clementine lines we used the technique of in situ gynogenesis induced by irradiated pollen. Flow cytometry, chromosome counts and SSR marker (Simple Sequence Repeats) analysis facilitated the identification of six different haploid lines (2n = x = 9), one aneuploid line (2n = 2x+4 = 22) and one doubled haploid plant (2n = 2x = 18) of 'Clemenules' clementine. One of the haploids, obtained directly from an original haploid embryo, grew vigorously and produced flowers after four years. This is the first haploid plant of clementine that has bloomed and we have, for the first time, characterized the histology of haploid and diploid flowers of clementine. Additionally a double haploid plant was obtained spontaneously from this haploid line. Conclusion. The first haploid plant of 'Clemenules' clementine produced directly by germination of a haploid embryo, which grew vigorously and produced flowers, has been obtained in this work. This haploid line has been selected and it is being used by the ICGC to establish the reference sequence of the nuclear genome of citrus. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/552434/1/document_552434.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1186/1471-2229-9-110 10.1186/1471-2229-9-110 info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2229-9-110 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/1471-2229-9-110 |
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F30 - Génétique et amélioration des plantes Citrus haploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_3490 http://aims.fao.org/aos/agrovoc/c_6ce991dd http://aims.fao.org/aos/agrovoc/c_7273 F30 - Génétique et amélioration des plantes Citrus haploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_3490 http://aims.fao.org/aos/agrovoc/c_6ce991dd http://aims.fao.org/aos/agrovoc/c_7273 |
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F30 - Génétique et amélioration des plantes Citrus haploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_3490 http://aims.fao.org/aos/agrovoc/c_6ce991dd http://aims.fao.org/aos/agrovoc/c_7273 F30 - Génétique et amélioration des plantes Citrus haploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_3490 http://aims.fao.org/aos/agrovoc/c_6ce991dd http://aims.fao.org/aos/agrovoc/c_7273 Aleza, Pablo Juarez, José Hernandez, Maria Pina, José Antonio Ollitrault, Patrick Navarro, Luis Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence |
description |
Background. In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus species are characterized by high heterozygosity, making it difficult to assemble large genome sequences. Thus, the International Citrus Genomic Consortium (ICGC) decided to establish a reference whole citrus genome sequence from a homozygous plant. Due to the existence of important molecular resources and previous success in obtaining haploid clementine plants, haploid clementine was selected as the target for the implementation of the reference whole genome citrus sequence. Results. To obtain haploid clementine lines we used the technique of in situ gynogenesis induced by irradiated pollen. Flow cytometry, chromosome counts and SSR marker (Simple Sequence Repeats) analysis facilitated the identification of six different haploid lines (2n = x = 9), one aneuploid line (2n = 2x+4 = 22) and one doubled haploid plant (2n = 2x = 18) of 'Clemenules' clementine. One of the haploids, obtained directly from an original haploid embryo, grew vigorously and produced flowers after four years. This is the first haploid plant of clementine that has bloomed and we have, for the first time, characterized the histology of haploid and diploid flowers of clementine. Additionally a double haploid plant was obtained spontaneously from this haploid line. Conclusion. The first haploid plant of 'Clemenules' clementine produced directly by germination of a haploid embryo, which grew vigorously and produced flowers, has been obtained in this work. This haploid line has been selected and it is being used by the ICGC to establish the reference sequence of the nuclear genome of citrus. |
format |
article |
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F30 - Génétique et amélioration des plantes Citrus haploïdie Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_3490 http://aims.fao.org/aos/agrovoc/c_6ce991dd http://aims.fao.org/aos/agrovoc/c_7273 |
author |
Aleza, Pablo Juarez, José Hernandez, Maria Pina, José Antonio Ollitrault, Patrick Navarro, Luis |
author_facet |
Aleza, Pablo Juarez, José Hernandez, Maria Pina, José Antonio Ollitrault, Patrick Navarro, Luis |
author_sort |
Aleza, Pablo |
title |
Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence |
title_short |
Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence |
title_full |
Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence |
title_fullStr |
Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence |
title_full_unstemmed |
Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence |
title_sort |
recovery and characterization of a citrus clementina hort. ex tan. 'clemenules' haploid plant selected to establish the reference whole citrus genome sequence |
url |
http://agritrop.cirad.fr/552434/ http://agritrop.cirad.fr/552434/1/document_552434.pdf |
work_keys_str_mv |
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