Production of tetraploid plants of non apomictic citrus genotypes
Ploidy manipulation in Citrus is a major issue of current breeding programs aiming to develop triploid seedless mandarins to address consumer demands for seedless fruits. The most effective method to obtain triploid hybrids is to pollinate tetraploid non apomictic cultivars with pollen of diploid varieties. Such non apomictic tetraploid lines are not found in the citrus germplasm and need to be created. In this work we describe a new methodology based on in vitro shoot-tip grafting combined with treatment of the micro-grafted shoot-tip with colchicine and oryzalin to achieve chromosome doubling and a dechimerization procedure assisted by flow cytometry. Stable tetraploid plants of Clemenules, Fina and Marisol clementines and Moncada mandarin have been obtained directly from shoot tip grafting combined with colchicine and oryzalin treatments or after dechimerization of mixoploids plants (2x-4x). These stable tetraploid plants have been used in 4x 9 2x hybridizations, to recover over 3,250 triploid hybrids in 3 years.
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Subjects: | F30 - Génétique et amélioration des plantes, Citrus, Citrus unshiu, Citrus clementina, http://aims.fao.org/aos/agrovoc/c_1637, http://aims.fao.org/aos/agrovoc/c_35538, http://aims.fao.org/aos/agrovoc/c_6ce991dd, http://aims.fao.org/aos/agrovoc/c_7273, |
Online Access: | http://agritrop.cirad.fr/552175/ http://agritrop.cirad.fr/552175/1/document_552175.pdf |
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dig-cirad-fr-5521752022-12-30T18:31:26Z http://agritrop.cirad.fr/552175/ http://agritrop.cirad.fr/552175/ Production of tetraploid plants of non apomictic citrus genotypes. Aleza Gil Pablo, Juarez José, Ollitrault Patrick, Navarro Luis. 2009. Plant Cell Reports, 28 (12) : 1837-1846.https://doi.org/10.1007/s00299-009-0783-2 <https://doi.org/10.1007/s00299-009-0783-2> Researchers Production of tetraploid plants of non apomictic citrus genotypes Aleza Gil, Pablo Juarez, José Ollitrault, Patrick Navarro, Luis eng 2009 Plant Cell Reports F30 - Génétique et amélioration des plantes Citrus Citrus unshiu Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_35538 http://aims.fao.org/aos/agrovoc/c_6ce991dd Espagne http://aims.fao.org/aos/agrovoc/c_7273 Ploidy manipulation in Citrus is a major issue of current breeding programs aiming to develop triploid seedless mandarins to address consumer demands for seedless fruits. The most effective method to obtain triploid hybrids is to pollinate tetraploid non apomictic cultivars with pollen of diploid varieties. Such non apomictic tetraploid lines are not found in the citrus germplasm and need to be created. In this work we describe a new methodology based on in vitro shoot-tip grafting combined with treatment of the micro-grafted shoot-tip with colchicine and oryzalin to achieve chromosome doubling and a dechimerization procedure assisted by flow cytometry. Stable tetraploid plants of Clemenules, Fina and Marisol clementines and Moncada mandarin have been obtained directly from shoot tip grafting combined with colchicine and oryzalin treatments or after dechimerization of mixoploids plants (2x-4x). These stable tetraploid plants have been used in 4x 9 2x hybridizations, to recover over 3,250 triploid hybrids in 3 years. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/552175/1/document_552175.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s00299-009-0783-2 10.1007/s00299-009-0783-2 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-009-0783-2 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s00299-009-0783-2 |
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F30 - Génétique et amélioration des plantes Citrus Citrus unshiu Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_35538 http://aims.fao.org/aos/agrovoc/c_6ce991dd http://aims.fao.org/aos/agrovoc/c_7273 F30 - Génétique et amélioration des plantes Citrus Citrus unshiu Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_35538 http://aims.fao.org/aos/agrovoc/c_6ce991dd http://aims.fao.org/aos/agrovoc/c_7273 |
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F30 - Génétique et amélioration des plantes Citrus Citrus unshiu Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_35538 http://aims.fao.org/aos/agrovoc/c_6ce991dd http://aims.fao.org/aos/agrovoc/c_7273 F30 - Génétique et amélioration des plantes Citrus Citrus unshiu Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_35538 http://aims.fao.org/aos/agrovoc/c_6ce991dd http://aims.fao.org/aos/agrovoc/c_7273 Aleza Gil, Pablo Juarez, José Ollitrault, Patrick Navarro, Luis Production of tetraploid plants of non apomictic citrus genotypes |
description |
Ploidy manipulation in Citrus is a major issue of current breeding programs aiming to develop triploid seedless mandarins to address consumer demands for seedless fruits. The most effective method to obtain triploid hybrids is to pollinate tetraploid non apomictic cultivars with pollen of diploid varieties. Such non apomictic tetraploid lines are not found in the citrus germplasm and need to be created. In this work we describe a new methodology based on in vitro shoot-tip grafting combined with treatment of the micro-grafted shoot-tip with colchicine and oryzalin to achieve chromosome doubling and a dechimerization procedure assisted by flow cytometry. Stable tetraploid plants of Clemenules, Fina and Marisol clementines and Moncada mandarin have been obtained directly from shoot tip grafting combined with colchicine and oryzalin treatments or after dechimerization of mixoploids plants (2x-4x). These stable tetraploid plants have been used in 4x 9 2x hybridizations, to recover over 3,250 triploid hybrids in 3 years. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Citrus Citrus unshiu Citrus clementina http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_35538 http://aims.fao.org/aos/agrovoc/c_6ce991dd http://aims.fao.org/aos/agrovoc/c_7273 |
author |
Aleza Gil, Pablo Juarez, José Ollitrault, Patrick Navarro, Luis |
author_facet |
Aleza Gil, Pablo Juarez, José Ollitrault, Patrick Navarro, Luis |
author_sort |
Aleza Gil, Pablo |
title |
Production of tetraploid plants of non apomictic citrus genotypes |
title_short |
Production of tetraploid plants of non apomictic citrus genotypes |
title_full |
Production of tetraploid plants of non apomictic citrus genotypes |
title_fullStr |
Production of tetraploid plants of non apomictic citrus genotypes |
title_full_unstemmed |
Production of tetraploid plants of non apomictic citrus genotypes |
title_sort |
production of tetraploid plants of non apomictic citrus genotypes |
url |
http://agritrop.cirad.fr/552175/ http://agritrop.cirad.fr/552175/1/document_552175.pdf |
work_keys_str_mv |
AT alezagilpablo productionoftetraploidplantsofnonapomicticcitrusgenotypes AT juarezjose productionoftetraploidplantsofnonapomicticcitrusgenotypes AT ollitraultpatrick productionoftetraploidplantsofnonapomicticcitrusgenotypes AT navarroluis productionoftetraploidplantsofnonapomicticcitrusgenotypes |
_version_ |
1758022251836342272 |