Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila
Maize streak virus (MSV, Mastrevirus, Geminiviridae) is persistently transmitted by Cicadulina mbila, apparently without propagation in its leafhopper vector. MSV was shown earlier by quantitative PCR to accumulate in the alimentary canal of C. mbila. We examined the alimentary canals of C. mbila leafhoppers that acquired MSV from diseased plants for various acquisition access periods (AAP) by immunofluorescence confocal laser scanning microscopy (iCLSM) and by immunogold labelling transmission electron microscopy (iTEM). Following a 7-day AAP and a 7-day inoculation period (IP) on healthy seedlings, MSV was detected by iCLSM mainly in the filter chamber and anterior midgut. Using iTEM, large accumulations of MSV particles, usually enclosed in membranous vesicles, were detected only in cells of the midgut, inside and outside the filter chamber, following 14- or 30-day AAPs, and also following 7-day AAP and 7-day IP on healthy plants. No virus was detected in the control non-vector species C. chinaï. Coated pits or vesicles, typical of clathrin-mediated endocytosis, were not observed. We discuss an alternative endocytosis pathway and suggest that the MSV accumulations are stored in endosomes in the midgut epithelial cells.
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Subjects: | H20 - Maladies des plantes, Zea mays, Cicadulina, virus striure du maïs, vecteur de maladie, Geminiviridae, hôte, http://aims.fao.org/aos/agrovoc/c_8504, http://aims.fao.org/aos/agrovoc/c_1610, http://aims.fao.org/aos/agrovoc/c_33375, http://aims.fao.org/aos/agrovoc/c_8164, http://aims.fao.org/aos/agrovoc/c_32859, http://aims.fao.org/aos/agrovoc/c_3673, http://aims.fao.org/aos/agrovoc/c_6543, http://aims.fao.org/aos/agrovoc/c_3081, |
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dig-cirad-fr-5486192024-01-28T16:54:30Z http://agritrop.cirad.fr/548619/ http://agritrop.cirad.fr/548619/ Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila. Ammar El-Desouky, Gargani Daniel, Lett Jean-Michel, Peterschmitt Michel. 2009. Archives of Virology, 154 (2) : 255-262.https://doi.org/10.1007/s00705-008-0308-2 <https://doi.org/10.1007/s00705-008-0308-2> Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila Ammar, El-Desouky Gargani, Daniel Lett, Jean-Michel Peterschmitt, Michel eng 2009 Archives of Virology H20 - Maladies des plantes Zea mays Cicadulina virus striure du maïs vecteur de maladie Geminiviridae hôte http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_1610 http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3673 La Réunion France http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Maize streak virus (MSV, Mastrevirus, Geminiviridae) is persistently transmitted by Cicadulina mbila, apparently without propagation in its leafhopper vector. MSV was shown earlier by quantitative PCR to accumulate in the alimentary canal of C. mbila. We examined the alimentary canals of C. mbila leafhoppers that acquired MSV from diseased plants for various acquisition access periods (AAP) by immunofluorescence confocal laser scanning microscopy (iCLSM) and by immunogold labelling transmission electron microscopy (iTEM). Following a 7-day AAP and a 7-day inoculation period (IP) on healthy seedlings, MSV was detected by iCLSM mainly in the filter chamber and anterior midgut. Using iTEM, large accumulations of MSV particles, usually enclosed in membranous vesicles, were detected only in cells of the midgut, inside and outside the filter chamber, following 14- or 30-day AAPs, and also following 7-day AAP and 7-day IP on healthy plants. No virus was detected in the control non-vector species C. chinaï. Coated pits or vesicles, typical of clathrin-mediated endocytosis, were not observed. We discuss an alternative endocytosis pathway and suggest that the MSV accumulations are stored in endosomes in the midgut epithelial cells. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/548619/1/document_548619.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s00705-008-0308-2 10.1007/s00705-008-0308-2 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=203562 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00705-008-0308-2 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s00705-008-0308-2 |
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H20 - Maladies des plantes Zea mays Cicadulina virus striure du maïs vecteur de maladie Geminiviridae hôte http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_1610 http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3673 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Zea mays Cicadulina virus striure du maïs vecteur de maladie Geminiviridae hôte http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_1610 http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3673 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
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H20 - Maladies des plantes Zea mays Cicadulina virus striure du maïs vecteur de maladie Geminiviridae hôte http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_1610 http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3673 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Zea mays Cicadulina virus striure du maïs vecteur de maladie Geminiviridae hôte http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_1610 http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3673 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Ammar, El-Desouky Gargani, Daniel Lett, Jean-Michel Peterschmitt, Michel Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila |
description |
Maize streak virus (MSV, Mastrevirus, Geminiviridae) is persistently transmitted by Cicadulina mbila, apparently without propagation in its leafhopper vector. MSV was shown earlier by quantitative PCR to accumulate in the alimentary canal of C. mbila. We examined the alimentary canals of C. mbila leafhoppers that acquired MSV from diseased plants for various acquisition access periods (AAP) by immunofluorescence confocal laser scanning microscopy (iCLSM) and by immunogold labelling transmission electron microscopy (iTEM). Following a 7-day AAP and a 7-day inoculation period (IP) on healthy seedlings, MSV was detected by iCLSM mainly in the filter chamber and anterior midgut. Using iTEM, large accumulations of MSV particles, usually enclosed in membranous vesicles, were detected only in cells of the midgut, inside and outside the filter chamber, following 14- or 30-day AAPs, and also following 7-day AAP and 7-day IP on healthy plants. No virus was detected in the control non-vector species C. chinaï. Coated pits or vesicles, typical of clathrin-mediated endocytosis, were not observed. We discuss an alternative endocytosis pathway and suggest that the MSV accumulations are stored in endosomes in the midgut epithelial cells. |
format |
article |
topic_facet |
H20 - Maladies des plantes Zea mays Cicadulina virus striure du maïs vecteur de maladie Geminiviridae hôte http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_1610 http://aims.fao.org/aos/agrovoc/c_33375 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3673 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Ammar, El-Desouky Gargani, Daniel Lett, Jean-Michel Peterschmitt, Michel |
author_facet |
Ammar, El-Desouky Gargani, Daniel Lett, Jean-Michel Peterschmitt, Michel |
author_sort |
Ammar, El-Desouky |
title |
Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila |
title_short |
Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila |
title_full |
Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila |
title_fullStr |
Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila |
title_full_unstemmed |
Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila |
title_sort |
large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector cicadulina mbila |
url |
http://agritrop.cirad.fr/548619/ http://agritrop.cirad.fr/548619/1/document_548619.pdf |
work_keys_str_mv |
AT ammareldesouky largeaccumulationsofmaizestreakvirusinthefilterchamberandmidgutcellsoftheleafhoppervectorcicadulinambila AT garganidaniel largeaccumulationsofmaizestreakvirusinthefilterchamberandmidgutcellsoftheleafhoppervectorcicadulinambila AT lettjeanmichel largeaccumulationsofmaizestreakvirusinthefilterchamberandmidgutcellsoftheleafhoppervectorcicadulinambila AT peterschmittmichel largeaccumulationsofmaizestreakvirusinthefilterchamberandmidgutcellsoftheleafhoppervectorcicadulinambila |
_version_ |
1792497156144234496 |