Amblyomma variegatum ticks and heartwater on three Caribbean Islands: Tick infection and ehrlichia ruminantium genetic diversity in bovine herds
Amblyomma variegatum tick infestation, tick infection by Ehrlichia ruminantium (ER), and ER genetic diversity were studied in the Caribbean Islands of Guadeloupe, Marie- Galante, and Antigua between 2003 and 2005. Nested PCR for pCS20 was used to detect ER,while ER strainswere characterized by sequencing or by restriction fragment length polymorphism (RFLP) profiles of map-1 PCR products. In 2003 in Guadeloupe, the prevalence of tick-infested herds was 35.6%. In Marie-Galante 79.1% of herds in 2003 and 73.8% in 2005 were infested, while only an average of 2.2% of the herds were infected in Antigua between this same period. InMarie-Galante, 19.1% of ticks were ER positive, and ER-infected ticks were found in 33.3% of the herds. In Antigua only 5.8% of the ticks were ER positive. High ER tick infection rate combined with a very high level of tick infestation highlight the risk of heartwater inMarie-Galante andGuadeloupe more than in Antigua. The three islands still represent a reservoir for tick and heartwater in the Caribbean. Nine different African and Caribbean map-1 ER genotypes were identified. This diversity was observed even in restricted areas, and identical map-1 genotypes were observed on all three islands. This high genetic diversity of ER strains suggests that there was a simultaneous introduction of several strains from African countries into the Caribbean region.
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | L73 - Maladies des animaux, Amblyomma variegatum, Ehrlichia, PCR, variation génétique, épidémiologie, Ehrlichia ruminantium, http://aims.fao.org/aos/agrovoc/c_23890, http://aims.fao.org/aos/agrovoc/c_2504, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_2615, http://aims.fao.org/aos/agrovoc/c_34694, http://aims.fao.org/aos/agrovoc/c_1320, http://aims.fao.org/aos/agrovoc/c_3406, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/547454/ http://agritrop.cirad.fr/547454/1/document_547454.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-547454 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5474542024-01-28T16:37:32Z http://agritrop.cirad.fr/547454/ http://agritrop.cirad.fr/547454/ Amblyomma variegatum ticks and heartwater on three Caribbean Islands: Tick infection and ehrlichia ruminantium genetic diversity in bovine herds. Vachiery Nathalie, Jeffery Helena, Pegram Rupert G., Aprelon Rosalie, Pinarello Valérie, Kandassamy Yane, Raliniaina Modestine, Molia Sophie, Savage Hazel, Alexander Randolph, Frebling Mathieu, Martinez Dominique, Lefrançois Thierry. 2008. Annals of the New York Academy of Sciences, 1149 : 191-195. Biennial Conference of the Society for Tropical Veterinary Medicine. 9, Mexico, Mexique, 17 Juin 2007/22 Juin 2007.https://doi.org/10.1196/annals.1428.081 <https://doi.org/10.1196/annals.1428.081> Amblyomma variegatum ticks and heartwater on three Caribbean Islands: Tick infection and ehrlichia ruminantium genetic diversity in bovine herds Vachiery, Nathalie Jeffery, Helena Pegram, Rupert G. Aprelon, Rosalie Pinarello, Valérie Kandassamy, Yane Raliniaina, Modestine Molia, Sophie Savage, Hazel Alexander, Randolph Frebling, Mathieu Martinez, Dominique Lefrançois, Thierry eng 2008 Annals of the New York Academy of Sciences L73 - Maladies des animaux Amblyomma variegatum Ehrlichia PCR variation génétique épidémiologie Ehrlichia ruminantium http://aims.fao.org/aos/agrovoc/c_23890 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_34694 Caraïbes Guadeloupe France http://aims.fao.org/aos/agrovoc/c_1320 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 Amblyomma variegatum tick infestation, tick infection by Ehrlichia ruminantium (ER), and ER genetic diversity were studied in the Caribbean Islands of Guadeloupe, Marie- Galante, and Antigua between 2003 and 2005. Nested PCR for pCS20 was used to detect ER,while ER strainswere characterized by sequencing or by restriction fragment length polymorphism (RFLP) profiles of map-1 PCR products. In 2003 in Guadeloupe, the prevalence of tick-infested herds was 35.6%. In Marie-Galante 79.1% of herds in 2003 and 73.8% in 2005 were infested, while only an average of 2.2% of the herds were infected in Antigua between this same period. InMarie-Galante, 19.1% of ticks were ER positive, and ER-infected ticks were found in 33.3% of the herds. In Antigua only 5.8% of the ticks were ER positive. High ER tick infection rate combined with a very high level of tick infestation highlight the risk of heartwater inMarie-Galante andGuadeloupe more than in Antigua. The three islands still represent a reservoir for tick and heartwater in the Caribbean. Nine different African and Caribbean map-1 ER genotypes were identified. This diversity was observed even in restricted areas, and identical map-1 genotypes were observed on all three islands. This high genetic diversity of ER strains suggests that there was a simultaneous introduction of several strains from African countries into the Caribbean region. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/547454/1/document_547454.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1196/annals.1428.081 10.1196/annals.1428.081 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=202898 info:eu-repo/semantics/altIdentifier/doi/10.1196/annals.1428.081 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1196/annals.1428.081 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
L73 - Maladies des animaux Amblyomma variegatum Ehrlichia PCR variation génétique épidémiologie Ehrlichia ruminantium http://aims.fao.org/aos/agrovoc/c_23890 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_34694 http://aims.fao.org/aos/agrovoc/c_1320 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 L73 - Maladies des animaux Amblyomma variegatum Ehrlichia PCR variation génétique épidémiologie Ehrlichia ruminantium http://aims.fao.org/aos/agrovoc/c_23890 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_34694 http://aims.fao.org/aos/agrovoc/c_1320 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 |
spellingShingle |
L73 - Maladies des animaux Amblyomma variegatum Ehrlichia PCR variation génétique épidémiologie Ehrlichia ruminantium http://aims.fao.org/aos/agrovoc/c_23890 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_34694 http://aims.fao.org/aos/agrovoc/c_1320 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 L73 - Maladies des animaux Amblyomma variegatum Ehrlichia PCR variation génétique épidémiologie Ehrlichia ruminantium http://aims.fao.org/aos/agrovoc/c_23890 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_34694 http://aims.fao.org/aos/agrovoc/c_1320 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 Vachiery, Nathalie Jeffery, Helena Pegram, Rupert G. Aprelon, Rosalie Pinarello, Valérie Kandassamy, Yane Raliniaina, Modestine Molia, Sophie Savage, Hazel Alexander, Randolph Frebling, Mathieu Martinez, Dominique Lefrançois, Thierry Amblyomma variegatum ticks and heartwater on three Caribbean Islands: Tick infection and ehrlichia ruminantium genetic diversity in bovine herds |
description |
Amblyomma variegatum tick infestation, tick infection by Ehrlichia ruminantium (ER), and ER genetic diversity were studied in the Caribbean Islands of Guadeloupe, Marie- Galante, and Antigua between 2003 and 2005. Nested PCR for pCS20 was used to detect ER,while ER strainswere characterized by sequencing or by restriction fragment length polymorphism (RFLP) profiles of map-1 PCR products. In 2003 in Guadeloupe, the prevalence of tick-infested herds was 35.6%. In Marie-Galante 79.1% of herds in 2003 and 73.8% in 2005 were infested, while only an average of 2.2% of the herds were infected in Antigua between this same period. InMarie-Galante, 19.1% of ticks were ER positive, and ER-infected ticks were found in 33.3% of the herds. In Antigua only 5.8% of the ticks were ER positive. High ER tick infection rate combined with a very high level of tick infestation highlight the risk of heartwater inMarie-Galante andGuadeloupe more than in Antigua. The three islands still represent a reservoir for tick and heartwater in the Caribbean. Nine different African and Caribbean map-1 ER genotypes were identified. This diversity was observed even in restricted areas, and identical map-1 genotypes were observed on all three islands. This high genetic diversity of ER strains suggests that there was a simultaneous introduction of several strains from African countries into the Caribbean region. |
format |
article |
topic_facet |
L73 - Maladies des animaux Amblyomma variegatum Ehrlichia PCR variation génétique épidémiologie Ehrlichia ruminantium http://aims.fao.org/aos/agrovoc/c_23890 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_34694 http://aims.fao.org/aos/agrovoc/c_1320 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Vachiery, Nathalie Jeffery, Helena Pegram, Rupert G. Aprelon, Rosalie Pinarello, Valérie Kandassamy, Yane Raliniaina, Modestine Molia, Sophie Savage, Hazel Alexander, Randolph Frebling, Mathieu Martinez, Dominique Lefrançois, Thierry |
author_facet |
Vachiery, Nathalie Jeffery, Helena Pegram, Rupert G. Aprelon, Rosalie Pinarello, Valérie Kandassamy, Yane Raliniaina, Modestine Molia, Sophie Savage, Hazel Alexander, Randolph Frebling, Mathieu Martinez, Dominique Lefrançois, Thierry |
author_sort |
Vachiery, Nathalie |
title |
Amblyomma variegatum ticks and heartwater on three Caribbean Islands: Tick infection and ehrlichia ruminantium genetic diversity in bovine herds |
title_short |
Amblyomma variegatum ticks and heartwater on three Caribbean Islands: Tick infection and ehrlichia ruminantium genetic diversity in bovine herds |
title_full |
Amblyomma variegatum ticks and heartwater on three Caribbean Islands: Tick infection and ehrlichia ruminantium genetic diversity in bovine herds |
title_fullStr |
Amblyomma variegatum ticks and heartwater on three Caribbean Islands: Tick infection and ehrlichia ruminantium genetic diversity in bovine herds |
title_full_unstemmed |
Amblyomma variegatum ticks and heartwater on three Caribbean Islands: Tick infection and ehrlichia ruminantium genetic diversity in bovine herds |
title_sort |
amblyomma variegatum ticks and heartwater on three caribbean islands: tick infection and ehrlichia ruminantium genetic diversity in bovine herds |
url |
http://agritrop.cirad.fr/547454/ http://agritrop.cirad.fr/547454/1/document_547454.pdf |
work_keys_str_mv |
AT vachierynathalie amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT jefferyhelena amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT pegramrupertg amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT aprelonrosalie amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT pinarellovalerie amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT kandassamyyane amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT raliniainamodestine amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT moliasophie amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT savagehazel amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT alexanderrandolph amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT freblingmathieu amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT martinezdominique amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds AT lefrancoisthierry amblyommavariegatumticksandheartwateronthreecaribbeanislandstickinfectionandehrlichiaruminantiumgeneticdiversityinbovineherds |
_version_ |
1792497075862110208 |