Vector competence of some french Culex and Aedes mosquitoes for west nile virus
To identify the mosquito species able to sustain the transmission of West Nile Virus (WNV) in the Camargue region (the main WNV focus of southern France), we assessed the vector competence of Culex modestus and Culex pipiens, the most abundant bird-feeders, and Aedes caspius, the most abundant mammophilic species occasionally found engorged with avian blood. Female mosquitoes were exposed to the infectious meal (1010.3 plaque forming units (PFU)/mL) by membrane feeding, and hold at 26°C. After the incubation period, disseminated infection was assessed by WNV detection using an indirect fluorescent antibody assay (IFA) on head squashes, and the transmission rate was assessed by the presence of WNV RNA in salivary secretions with a real-time reverse transcriptase-polymerase chain reaction (RT-PCR). After 14 incubation days, the disseminated infection and the transmission rates were 89.2% and 54.5% for Cx. modestus, 38.5% and 15.8% for Cx. pipiens, and 0.8% and 0 for Ae. caspius. Culex modestus was found to be an extremely efficient laboratory WNV vector and could thus be considered the main WNV vector in wetlands of the Camargue. Culex pipiens was a moderately efficient laboratory WNV vector, but in dry areas of the region it could play the main role in WNV transmission between birds and from birds to mammals. Aedes caspius was an inefficient vector of WNV in the laboratory, and despite its high densities, its role in WNV transmission may be minor in southern France.
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Subjects: | L73 - Maladies des animaux, flavivirus, vecteur de maladie, identification, transmission des maladies, Culex, Culex pipiens, Aedes, infection expérimentale, http://aims.fao.org/aos/agrovoc/c_2963, http://aims.fao.org/aos/agrovoc/c_8164, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_2329, http://aims.fao.org/aos/agrovoc/c_2015, http://aims.fao.org/aos/agrovoc/c_30516, http://aims.fao.org/aos/agrovoc/c_146, http://aims.fao.org/aos/agrovoc/c_32441, http://aims.fao.org/aos/agrovoc/c_4188, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/546869/ http://agritrop.cirad.fr/546869/1/document_546869.pdf |
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dig-cirad-fr-5468692024-01-28T16:30:08Z http://agritrop.cirad.fr/546869/ http://agritrop.cirad.fr/546869/ Vector competence of some french Culex and Aedes mosquitoes for west nile virus. Balenghien Thomas, Vazeille Marie, Grandadam Marc, Schaffner Francis, Zeller Hervé, Reiter Paul, Sabatier Philippe, Fouque Florence, Bicout Dominique. 2008. Vector Borne and Zoonotic Diseases, 8 (5) : 589-595.https://doi.org/10.1089/vbz.2007.0266 <https://doi.org/10.1089/vbz.2007.0266> Vector competence of some french Culex and Aedes mosquitoes for west nile virus Balenghien, Thomas Vazeille, Marie Grandadam, Marc Schaffner, Francis Zeller, Hervé Reiter, Paul Sabatier, Philippe Fouque, Florence Bicout, Dominique eng 2008 Vector Borne and Zoonotic Diseases L73 - Maladies des animaux flavivirus vecteur de maladie identification transmission des maladies Culex Culex pipiens Aedes infection expérimentale http://aims.fao.org/aos/agrovoc/c_2963 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_2015 http://aims.fao.org/aos/agrovoc/c_30516 http://aims.fao.org/aos/agrovoc/c_146 http://aims.fao.org/aos/agrovoc/c_32441 Languedoc-Roussillon France http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 To identify the mosquito species able to sustain the transmission of West Nile Virus (WNV) in the Camargue region (the main WNV focus of southern France), we assessed the vector competence of Culex modestus and Culex pipiens, the most abundant bird-feeders, and Aedes caspius, the most abundant mammophilic species occasionally found engorged with avian blood. Female mosquitoes were exposed to the infectious meal (1010.3 plaque forming units (PFU)/mL) by membrane feeding, and hold at 26°C. After the incubation period, disseminated infection was assessed by WNV detection using an indirect fluorescent antibody assay (IFA) on head squashes, and the transmission rate was assessed by the presence of WNV RNA in salivary secretions with a real-time reverse transcriptase-polymerase chain reaction (RT-PCR). After 14 incubation days, the disseminated infection and the transmission rates were 89.2% and 54.5% for Cx. modestus, 38.5% and 15.8% for Cx. pipiens, and 0.8% and 0 for Ae. caspius. Culex modestus was found to be an extremely efficient laboratory WNV vector and could thus be considered the main WNV vector in wetlands of the Camargue. Culex pipiens was a moderately efficient laboratory WNV vector, but in dry areas of the region it could play the main role in WNV transmission between birds and from birds to mammals. Aedes caspius was an inefficient vector of WNV in the laboratory, and despite its high densities, its role in WNV transmission may be minor in southern France. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/546869/1/document_546869.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1089/vbz.2007.0266 10.1089/vbz.2007.0266 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=202523 info:eu-repo/semantics/altIdentifier/doi/10.1089/vbz.2007.0266 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1089/vbz.2007.0266 |
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L73 - Maladies des animaux flavivirus vecteur de maladie identification transmission des maladies Culex Culex pipiens Aedes infection expérimentale http://aims.fao.org/aos/agrovoc/c_2963 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_2015 http://aims.fao.org/aos/agrovoc/c_30516 http://aims.fao.org/aos/agrovoc/c_146 http://aims.fao.org/aos/agrovoc/c_32441 http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 L73 - Maladies des animaux flavivirus vecteur de maladie identification transmission des maladies Culex Culex pipiens Aedes infection expérimentale http://aims.fao.org/aos/agrovoc/c_2963 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_2015 http://aims.fao.org/aos/agrovoc/c_30516 http://aims.fao.org/aos/agrovoc/c_146 http://aims.fao.org/aos/agrovoc/c_32441 http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 |
spellingShingle |
L73 - Maladies des animaux flavivirus vecteur de maladie identification transmission des maladies Culex Culex pipiens Aedes infection expérimentale http://aims.fao.org/aos/agrovoc/c_2963 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_2015 http://aims.fao.org/aos/agrovoc/c_30516 http://aims.fao.org/aos/agrovoc/c_146 http://aims.fao.org/aos/agrovoc/c_32441 http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 L73 - Maladies des animaux flavivirus vecteur de maladie identification transmission des maladies Culex Culex pipiens Aedes infection expérimentale http://aims.fao.org/aos/agrovoc/c_2963 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_2015 http://aims.fao.org/aos/agrovoc/c_30516 http://aims.fao.org/aos/agrovoc/c_146 http://aims.fao.org/aos/agrovoc/c_32441 http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 Balenghien, Thomas Vazeille, Marie Grandadam, Marc Schaffner, Francis Zeller, Hervé Reiter, Paul Sabatier, Philippe Fouque, Florence Bicout, Dominique Vector competence of some french Culex and Aedes mosquitoes for west nile virus |
description |
To identify the mosquito species able to sustain the transmission of West Nile Virus (WNV) in the Camargue region (the main WNV focus of southern France), we assessed the vector competence of Culex modestus and Culex pipiens, the most abundant bird-feeders, and Aedes caspius, the most abundant mammophilic species occasionally found engorged with avian blood. Female mosquitoes were exposed to the infectious meal (1010.3 plaque forming units (PFU)/mL) by membrane feeding, and hold at 26°C. After the incubation period, disseminated infection was assessed by WNV detection using an indirect fluorescent antibody assay (IFA) on head squashes, and the transmission rate was assessed by the presence of WNV RNA in salivary secretions with a real-time reverse transcriptase-polymerase chain reaction (RT-PCR). After 14 incubation days, the disseminated infection and the transmission rates were 89.2% and 54.5% for Cx. modestus, 38.5% and 15.8% for Cx. pipiens, and 0.8% and 0 for Ae. caspius. Culex modestus was found to be an extremely efficient laboratory WNV vector and could thus be considered the main WNV vector in wetlands of the Camargue. Culex pipiens was a moderately efficient laboratory WNV vector, but in dry areas of the region it could play the main role in WNV transmission between birds and from birds to mammals. Aedes caspius was an inefficient vector of WNV in the laboratory, and despite its high densities, its role in WNV transmission may be minor in southern France. |
format |
article |
topic_facet |
L73 - Maladies des animaux flavivirus vecteur de maladie identification transmission des maladies Culex Culex pipiens Aedes infection expérimentale http://aims.fao.org/aos/agrovoc/c_2963 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_2015 http://aims.fao.org/aos/agrovoc/c_30516 http://aims.fao.org/aos/agrovoc/c_146 http://aims.fao.org/aos/agrovoc/c_32441 http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Balenghien, Thomas Vazeille, Marie Grandadam, Marc Schaffner, Francis Zeller, Hervé Reiter, Paul Sabatier, Philippe Fouque, Florence Bicout, Dominique |
author_facet |
Balenghien, Thomas Vazeille, Marie Grandadam, Marc Schaffner, Francis Zeller, Hervé Reiter, Paul Sabatier, Philippe Fouque, Florence Bicout, Dominique |
author_sort |
Balenghien, Thomas |
title |
Vector competence of some french Culex and Aedes mosquitoes for west nile virus |
title_short |
Vector competence of some french Culex and Aedes mosquitoes for west nile virus |
title_full |
Vector competence of some french Culex and Aedes mosquitoes for west nile virus |
title_fullStr |
Vector competence of some french Culex and Aedes mosquitoes for west nile virus |
title_full_unstemmed |
Vector competence of some french Culex and Aedes mosquitoes for west nile virus |
title_sort |
vector competence of some french culex and aedes mosquitoes for west nile virus |
url |
http://agritrop.cirad.fr/546869/ http://agritrop.cirad.fr/546869/1/document_546869.pdf |
work_keys_str_mv |
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_version_ |
1792497041594646528 |