A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm
An immunocapture (IC) One-step RT-PCR assay was developed to improve the detection of Banana bract mosaic virus (BBrMV) in single and bulked samples of banana plants. In this paper, an atypical strain of BBrMV was described, the BBrMV "Ref" strain, and we showed that detection with available BBrMV tools using ELISA and RT-PCR approacheswas not reliable. Primer sets Bract N1/NR and N2/NR specific to BBrMV were designed and used in RT-PCR and IC-RT-PCR assays with two commercial kits that allow the RT and the PCR reactions to take place simultaneously in the same tube. The new assay enabled detection of BBrMV in leaf extract diluted up to 1×10-10 and in bulked samples of 10 plants, and was proposed as a new international standard to index BBrMV.
Main Authors: | , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, Musa, virus des végétaux, PCR, Test ELISA, diagnostic, germoplasme, http://aims.fao.org/aos/agrovoc/c_4993, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_15968, http://aims.fao.org/aos/agrovoc/c_2238, http://aims.fao.org/aos/agrovoc/c_3249, |
Online Access: | http://agritrop.cirad.fr/546691/ http://agritrop.cirad.fr/546691/1/document_546691.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-546691 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5466912024-01-28T16:27:16Z http://agritrop.cirad.fr/546691/ http://agritrop.cirad.fr/546691/ A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm. Iskra Caruana Marie-Line, Galzi Serge, Laboureau Nathalie. 2008. Journal of Virological Methods, 153 (2) : 223-231.https://doi.org/10.1016/j.jviromet.2008.06.028 <https://doi.org/10.1016/j.jviromet.2008.06.028> A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm Iskra Caruana, Marie-Line Galzi, Serge Laboureau, Nathalie eng 2008 Journal of Virological Methods H20 - Maladies des plantes Musa virus des végétaux PCR Test ELISA diagnostic germoplasme http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_3249 An immunocapture (IC) One-step RT-PCR assay was developed to improve the detection of Banana bract mosaic virus (BBrMV) in single and bulked samples of banana plants. In this paper, an atypical strain of BBrMV was described, the BBrMV "Ref" strain, and we showed that detection with available BBrMV tools using ELISA and RT-PCR approacheswas not reliable. Primer sets Bract N1/NR and N2/NR specific to BBrMV were designed and used in RT-PCR and IC-RT-PCR assays with two commercial kits that allow the RT and the PCR reactions to take place simultaneously in the same tube. The new assay enabled detection of BBrMV in leaf extract diluted up to 1×10-10 and in bulked samples of 10 plants, and was proposed as a new international standard to index BBrMV. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/546691/1/document_546691.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.jviromet.2008.06.028 10.1016/j.jviromet.2008.06.028 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2008.06.028 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.jviromet.2008.06.028 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H20 - Maladies des plantes Musa virus des végétaux PCR Test ELISA diagnostic germoplasme http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_3249 H20 - Maladies des plantes Musa virus des végétaux PCR Test ELISA diagnostic germoplasme http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_3249 |
spellingShingle |
H20 - Maladies des plantes Musa virus des végétaux PCR Test ELISA diagnostic germoplasme http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_3249 H20 - Maladies des plantes Musa virus des végétaux PCR Test ELISA diagnostic germoplasme http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_3249 Iskra Caruana, Marie-Line Galzi, Serge Laboureau, Nathalie A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm |
description |
An immunocapture (IC) One-step RT-PCR assay was developed to improve the detection of Banana bract mosaic virus (BBrMV) in single and bulked samples of banana plants. In this paper, an atypical strain of BBrMV was described, the BBrMV "Ref" strain, and we showed that detection with available BBrMV tools using ELISA and RT-PCR approacheswas not reliable. Primer sets Bract N1/NR and N2/NR specific to BBrMV were designed and used in RT-PCR and IC-RT-PCR assays with two commercial kits that allow the RT and the PCR reactions to take place simultaneously in the same tube. The new assay enabled detection of BBrMV in leaf extract diluted up to 1×10-10 and in bulked samples of 10 plants, and was proposed as a new international standard to index BBrMV. |
format |
article |
topic_facet |
H20 - Maladies des plantes Musa virus des végétaux PCR Test ELISA diagnostic germoplasme http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_3249 |
author |
Iskra Caruana, Marie-Line Galzi, Serge Laboureau, Nathalie |
author_facet |
Iskra Caruana, Marie-Line Galzi, Serge Laboureau, Nathalie |
author_sort |
Iskra Caruana, Marie-Line |
title |
A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm |
title_short |
A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm |
title_full |
A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm |
title_fullStr |
A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm |
title_full_unstemmed |
A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm |
title_sort |
reliable ic one-step rt-pcr method for the detection of bbrmv to ensure safe exchange of musa germplasm |
url |
http://agritrop.cirad.fr/546691/ http://agritrop.cirad.fr/546691/1/document_546691.pdf |
work_keys_str_mv |
AT iskracaruanamarieline areliableiconesteprtpcrmethodforthedetectionofbbrmvtoensuresafeexchangeofmusagermplasm AT galziserge areliableiconesteprtpcrmethodforthedetectionofbbrmvtoensuresafeexchangeofmusagermplasm AT laboureaunathalie areliableiconesteprtpcrmethodforthedetectionofbbrmvtoensuresafeexchangeofmusagermplasm AT iskracaruanamarieline reliableiconesteprtpcrmethodforthedetectionofbbrmvtoensuresafeexchangeofmusagermplasm AT galziserge reliableiconesteprtpcrmethodforthedetectionofbbrmvtoensuresafeexchangeofmusagermplasm AT laboureaunathalie reliableiconesteprtpcrmethodforthedetectionofbbrmvtoensuresafeexchangeofmusagermplasm |
_version_ |
1792497025561919488 |